Investigating pH-Dependent Modulations in Haemoglobin Response to Linoleic Acid: A Spectroscopic Analysis

• Autorzy: , , Christiana Ikechukwu
• Języki publikacji: EN

Abstrakty

EN

This study investigates the pH-dependent modulations in haemoglobin response to linoleic acid, employing a spectroscopic analysis across HbAA, HbAS, and HbSS variants. Concentration-dependent effects of linoleic acid at varying pH levels reveal nuanced behaviours in absorbance, peak maintenance, and spectra characteristics. At pH 7.2, HbAA and HbSS display increased absorbance with peak maintenance, while HbAS exhibits gradual absorbance rise. pH 5.0 induces absorbance increase in all variants, with maintained peaks at 415nm and gradual decrease at the oxy-band region. Variants exhibit distinct spectra characteristics, emphasizing the need for tailored approaches in clinical management. Findings align with empirical literature, emphasizing haemoglobin's multifunctionality. Implications span clinical considerations, dietary influences, and broader insights into haemoglobin stability. Suggestions for future studies propose molecular explorations, disease-specific investigations, computational modelling integration, longitudinal studies, and intervention strategy explorations. This study contributes to the evolving understanding of haemoglobin responses to environmental factors, laying groundwork for personalized approaches in healthcare and dietary recommendations.

Słowa kluczowe

EN

Haemoglobin Response; Linoleic Acid; Ph-Dependent Modulation; Spectroscopic Analysis; Variant-Specific Response

• Wydawca: Scientific Publishing House „DARWIN”
• Czasopismo: World News of Natural Sciences
• Rocznik: 2024
• Tom: 53
• Strony: 231-243

Twórcy

autor

,

• Department of Biochemistry, Imo State University, Owerri, Nigeria

autor

Christiana Ikechukwu

• Department of Biochemistry, Imo State University, Owerri, Nigeria

Bibliografia

• [1] Ahmed, M. H., Ghatge, M. S., & Safo, M. K. (2020). Haemoglobin: Structure, Function and Allostery. Subcellular Biochemistry, 94, 345–382. https://doi.org/10.1007/978-3-030-41769-7_14
• [2] Albiti, A. H., & Nsiah, K. (2014). Comparative haematological parameters of HbAA and HbAS genotype children infected with Plasmodium falciparum malaria in Yemen. Hematology 19(3), 169–174. https://doi.org/10.1179/1607845413Y.0000000113
• [3] Chikezie, P. C. (2009). Comparative methaemoglobin concentrations of three erythrocyte genotypes (HbAA, HbAS and HbSS) of male participants administered with five antimalarial drugs. African Journal of Biochemistry Research, 3(6), 266–271. https://doi.org/10.5897/AJBR.9000062
• [4] Deller, M. C., Kong, L., & Rupp, B. (2016). Protein stability: a crystallographer’s perspective. Acta Crystallographica. Section F, Structural Biology Communications, 72(Pt 2), 72–95. https://doi.org/10.1107/S2053230X15024619
• [5] Ezebuo, F. C., Eze, S. O. O., & Chilaka, F. C. (2012). Effects of sodium dodecyl sulphate on enhancement of lipoxygenase activity of haemoglobin. Indian Journal of Experimental Biology, 50(12), 847–852. https://pubmed.ncbi.nlm.nih.gov/23986967/
• [6] Giardina, B. (2021). Haemoglobin: Multiple molecular interactions and multiple functions. An example of energy optimization and global molecular organization. Molecular Aspects of Medicine, 84, 101040. https://doi.org/10.1016/j.mam.2021.101040
• [7] Hamilton, J. S., & Klett, E. L. (2021). Linoleic acid and the regulation of glucose homeostasis: A review of the evidence. Prostaglandins, Leukotrienes and Essential Fatty Acids, 175, 102366. https://doi.org/10.1016/j.plefa.2021.102366
• [8] Harteveld, C. L., Achour, A., Arkesteijn, S. J. G., ter Huurne, J., Verschuren, M., Bhagwandien‐Bisoen, S., Schaap, R., Vijfhuizen, L., el Idrissi, H., & Koopmann, T. T. (2022). The haemoglobinopathies, molecular disease mechanisms and diagnostics. International Journal of Laboratory Hematology, 44(S1), 28–36. https://doi.org/10.1111/ijlh.13885
• [9] Izuwa, G., Akpotuzor, J. O., Okpokam, D. C., Akpan, P. A., Ernest, N. A., & Asuquo, J. (2015). Haemorrheologic and Fibrinolytic Activities of HbSS, HbAS and HbAA Subjects in Abuja, Nigeria. Journal of Medical Sciences, 16(1-2), 32–37. https://doi.org/10.3923/jms.2016.32.37
• [10] Nahavandi, M., Nichols, J., Mohabatkar, H., Gandjbakhche, A., & Kato, G. J. (2009). Near-infrared spectra absorbance of blood from sickle cell patients and normal individuals. Hematology, 14(1), 46–48. https://doi.org/10.1179/102453309x385133
• [11] Rajendran, D., & Chandrasekaran, N. (2023). Molecular Interaction of Functionalized Nanoplastics with Human Haemoglobin. Journal of Fluorescence, 33(6), 2257–2272. https://doi.org/10.1007/s10895-023-03221-3
• [12] Reinmuth-Selzle, K., Tchipilov, T., Backes, A. T., Tscheuschner, G., Tang, K., Ziegler, K., Lucas, K., Pöschl, U., Fröhlich-Nowoisky, J., & Weller, M. G. (2022). Determination of the protein content of complex samples by aromatic amino acid analysis, liquid chromatography-UV absorbance, and colorimetry. Analytical and Bioanalytical Chemistry, 414(15). https://doi.org/10.1007/s00216-022-03910-1
• [13] Sinha, S., Jeyaseelan, C., Singh, G., Munjal, T., Paul, D., Bhatt, A. K., Bhatia, R. K., & Bhalla, T. C. (2023). Chapter 8: Spectroscopy—Principle, types, and applications. In Basic Biotechniques for Bioprocess and Bioentrepreneurship (pp. 145–164). Academic Press. https://doi.org/10.1016/B9780128161098.000088
• [14] Whelan, J., & Fritsche, K. (2013). Linoleic Acid. Advances in Nutrition, 4(3), 311–312. https://doi.org/10.3945/an.113.003772
• [15] Yuan, T., Fan, W.-B., Cong, Y., Xu, H.-D., Li, C.-J., Meng, J., Bao, N.-R., & Zhao, J.-N. (2015). Linoleic acid induces red blood cells and haemoglobin damage via oxidative mechanism. International Journal of Clinical and Experimental Pathology, 8(5), 5044–5052

• Typ dokumentu: article