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2025 | 59 | 142-152
Tytuł artykułu

Development of HPLC method for estimation of β-sitosterol from Peliosanthes micrantha hizomes

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Języki publikacji
EN
Abstrakty
EN
The medicinal plant Peliosanthes micrantha of Vietnam has been studied recently. Two components, one of which was β-sitosterol, were isolated. P. micrantha rhizomes were first extracted by ethanol (PM-R-ET). β-sitosterol was then isolated as a standard compound. High-performance liquid chromatography method (HPLC) was validated and used for the quantification of β-sitosterol in the extract. We concluded in this study that β-sitosterol content was significant at 0.71 mg/g of fresh P. micrantha rhizome extract.
Rocznik
Tom
59
Strony
142-152
Opis fizyczny
Twórcy
autor
,,
  • Centre for High Technology Research and Development, Vietnam Academy of Science and Technology, Hoang Quoc Viet 18, 10000, Hanoi, Vietnam
autor
  • Centre for High Technology Research and Development, Vietnam Academy of Science and Technology, Hoang Quoc Viet 18, 10000, Hanoi, Vietnam
  • Centre for High Technology Research and Development, Vietnam Academy of Science and Technology, Hoang Quoc Viet 18, 10000, Hanoi, Vietnam
Bibliografia
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  • [2] Do Ngoc Thuy, Nguyen Thi Huong, Phung Van Trung, Le Ngoc Hung (2024). Study of Peliosanthes micrantha medicinal plant: UHPLC‐Q‐TOF‐MS analysis of phytochemicals and antioxidant activity. Journal of Food Sciences 6(1): 14-25. doi: 10.47941/jfs.2023
  • [3] Le Ngoc Hung, Do Ngoc Thuy, Tran Van Thanh, Nguyen Thi Huong (2024). Standardization of Peliosanthes micrantha extract and male wellness hard capsule. Journal of Vietnam Rural Development Sciences, V83, pages 44-50
  • [4] Le Ngoc Hung, Tran Van Thanh, Nguyen Duc Anh, Dai Thi Viet Lan (2024). Evaluate the acute and sub-chronic toxicities of the Ageless Man capsule on the experimental animals. Journal of Analytical Sciences. 1 (30), p.51-56 (in Vietnamese).
  • [5] Xu, Xinya; Xie, Haihui; Hao, Jing; Jiang, Yueming; Wei, Xiaoyi (2010). Eudesmane sesquiterpene glucosides from lychee seed and their cytotoxic activity. Food chemistry. Vol. 123 (4), p. 1123-1126
  • [6] Lin CC, Chung YC, Hsu CP (2013). Anti-cancer potential of litchi seed extract. World J Exp Med. 3(4): 56-61. DOI: 10.5493/wjem.v3.i4.56
  • [7] Kitajima J, Kimizuka K, Tanaka Y (2000). Three new sesquiterpenoid glucosides of Ficus pumila fruit. Chem Pharm Bull (Tokyo), 48(1): 77-80. doi: 10.1248/cpb.48.77
  • [8] Qi, Z.-Y., Zhao, J.-Y., Lin, F.-J., Zhou, W.-L., & Gan, R.-Y. (2021). Bioactive Compounds, Therapeutic Activities, and Applications of Ficus pumila L. Agronomy. 11(1), 89. https://doi.org/10.3390/agronomy11010089
  • [9] Anwar, M. A., Tabassam, S., Gulfraz, M., Sheeraz Ahmad, M., Raja, G. K., & Arshad, M. (2020). Isolation of oxyberberine and β-sitosterol from Berberis lycium Royle root bark extract and in vitro cytotoxicity against liver and lung cancer cell lines. Evidence-Based Complementary and Alternative Medicine Volume 2020, Article ID 2596082, 9 pages. https://doi.org/10.1155/2020/2596082
  • [10] Khanam, S.; Sultana, R. (2012). Isolation of β-sitosterol & stigmasterol as active immunomodulatory constituents from fruits of Solanum xanthocarpum (Solanaceae). International Journal of Pharmaceutical Sciences and Research 3, 1057–1060
  • [11] Pateh, U.; Haruna, A.; Garba, M.; Iliya, I.; Sule, I.; Abubakar, M.; Ambi, A (2009). Isolation of stigmasterol, β-sitosterol and 2-hydroxyhexadecanoic acid methyl ester from the rhizomes of Stylochiton lancifolius Pyer and Kotchy (Araceae). Niger. J. Pharm. Sci. 7, 19–25
  • [12] Kitajima, J.; Kimizuka, K.; Arai, M.; Tanaka, Y. (1998). Constituents of Ficus pumila leaves. Chem. Pharm. Bull. 46, 1647–1649
  • [13] Xiao, W.; Chen, W.; Song, X.; Chen, G.; Zhang, J.; Liu, L.; Han, C (2015). Chemical constituents from the stems of Ficus pumila. Chin. Tradit. Pat. Med. 37, 1734–1737.
  • [14] Durrani, A. K., Khalid, M., Raza, A., Faiz ul Rasool, I., Khalid, W., Akhtar, M. N., …. Khadijah, B. (2024). Clinical improvement, toxicity and future prospects of β-sitosterol: a review. CyTA - Journal of Food, 22(1). 1-10. https://doi.org/10.1080/19476337.2024.2337886
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  • [16] Patidar, T., Ramteke, S (2024). Development and Validation of a Robust HPLC Method for Simultaneous Quantitative Analysis of Quercetin and β-sitosterol in Plant Extract. Food Anal. Methods. 17, 393–405. https://doi.org/10.1007/s12161-024-02581-1
  • [17] Lee DG, Lee J, Kim KT, Lee SW, Kim YO, Cho IH, Kim HJ, Park CG, Lee S. (2018). High-performance liquid chromatography analysis of phytosterols in Panax ginseng root grown under different conditions. J Ginseng Res. 42(1): 16-20. doi: 10.1016/j.jgr.2016.10.004
  • [18] Nittaya Ngamkhae, Yaowared Chulikhit, Orawan Monthakantirat, Juthamart Maneenet, Charinya Khamphukdee, Chantana Boonyarat, Supawadee Daodee (2022). Development and Validation of a High-performance Liquid Chromatography Method for Simultaneous Determination of Five Active Compounds in Kleeb Bua Daeng Formula. Research Journal of Pharmacy and Technology. 15(8): 3618-6. doi: 10.52711/0974-360X.2022.00606
  • [19] Sofia Chanioti, Maria Katsouli, and Constantina Tzia (2021). Chapter 9 - β-Sitosterol as a functional bioactive. Editor(s): Muhammad Mushtaq, Farooq Anwar. A Centum of Valuable Plant Bioactives. Academic Press. Pages 193-212. https://doi.org/10.1016/B978-0-12-822923-1.00014-5
Typ dokumentu
article
Bibliografia
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Identyfikator YADDA
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