Short-chain oligosaccharide synthesis by endo-beta-1,3 gluconase from Oerskovia xanthineolytica

• Autorzy: S. Bielecki , A. Bukowiecka
• Języki publikacji: EN

Abstrakty

EN

An approach for enzymatic sythesis of oligosachcharides via transglycosylation with endo-beta-1,3-glucanase from Oerskovia xanthineolytica is described.Linear -beta-1,3-glucan (laminarin) was used as a donor of glycosyl residues, whereas nitrophenyl glycosides of different monosaccharides served as acceptors.The synthesis was performed in water-organic solvent environment with several combinations of donor/acceptor.Employing p-nitrophenyl-beta-D-xylopyranoside as an acceptor in the presence of 30% acetonitrile resulted in the production of six new glycosides shown by FAB MS to be di-, tri- and tetrasaccharides.This enzyme is therefore suitable for the synthesis of short-chain oligosaccharides.

Słowa kluczowe

EN

ENDO-BETA-1,3-GLUCANASE; OERSKOVIA XANTHINEOLYTICA; OLIGOSACCHARIDE

• Czasopismo: Biotechnologia
• Rocznik: 1996
• Numer: 2
• Strony: 184-191

Twórcy

autor

S. Bielecki

autor

A. Bukowiecka

• Typ dokumentu: article
• Bibliografia: S.Bielecki