Screening for mutations affecting sexual reproduction after activation tagging in Arabidopsis thaliana

• Autorzy: G. Perrella , G. Cremona , F. Consiglio , A. Errico , R.A. Bressan , C. Conicella
• Języki publikacji: EN

Abstrakty

EN

In this work, a seed-set-based screening was performed on 70 lines of Arabidopsis thaliana after activation tagging mutagenesis to identify mutations in reproductive mechanisms. Five mutants showed significantly lower seed set than the wild type and confirmed the phenotype in the progeny. This phenotype was linked with the marker gene bar carried by T-DNA conferring glufosinate resistance. Genetic analysis revealed that the mutation inheritance was sporophytic in 3 mutants and gametophytic in 2 mutants. In addition, 2 mutants had an extra T-DNA copy. Thus activation tagging can be an effective strategy to identify new mutations affecting sporogenesis or gametogenesis.

Słowa kluczowe

EN

ACTIVATION TAGGING; ARABIDOPSIS THALIANA; GAMETOPHYTIC MUTATION; SEXUAL REPRODUCTION; SPOROPHYTIC MUTATION; T-DNA

• Czasopismo: Journal of Applied Genetics
• Rocznik: 2006
• Tom: 47
• Numer: 2
• Strony: 109-111

Twórcy

autor

G. Perrella

autor

G. Cremona

autor

F. Consiglio

autor

A. Errico

autor

R.A. Bressan

autor

C. Conicella

• Typ dokumentu: ARTICLE
• Bibliografia: C. Conicella, CNR-IGV, Institute of Plant Genetics, Research Division Portici, Via Universita 133, 80055 Portici, Italy