Polymorphism of the porcine growth hormone gene and its linkage to hormone gene and its linkage to microsatellites S0083 and S0090

• Autorzy: A. Korwin-Kossakowska , M. Pierzchala , J. Kuryl , L. Zwierzchowski , I. Cymerowska-Prokopczyk , SiadkowskaE.
• Języki publikacji: EN

Abstrakty

EN

Polymorphism of microsatellites S0083 and S0090 as well as of the second exon and second intron of the pGH (porcine growth hormone) gene was determined for 293 pedigrees obtained from crossing of 12 F1 (Zlotnicka Spotted x Polish Large White) boars with 64 F1 (Zlotnicka Spotted x Polish Large White) sows, experimental material arranged for a QTL mapping project. Microsatellites were genotyped by capillary electrophoresis of PCR products in an ABI PRISM 310 Genetic Analyser PERKIN-ELMER. The PCR-RFLP polymorphism of the GH gene was identified using HaeII and MspI restriction endonucleases. The following order of linked loci was established: GH-S0083-S0090. This is important information for the mapping of QTLs on chromosome 12.

Słowa kluczowe

EN

GENE POLYMORPHISM; LINKAGE; MICROSATELLITE; PIG; SUS SCROFA

• Czasopismo: Journal of Applied Genetics
• Rocznik: 1999
• Tom: 40
• Numer: 2
• Strony: 85-91

Twórcy

autor

A. Korwin-Kossakowska

autor

M. Pierzchala

autor

J. Kuryl

autor

L. Zwierzchowski

autor

I. Cymerowska-Prokopczyk

autor

SiadkowskaE.

• Bibliografia: J. Kuryl, Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzebiec, 05-551 Mrokow, Poland