Patterned growth and differentiation of human cord blood- derived neural stem cells on bio-functionalized surfaces

• Autorzy: L. Buzanska , A. Ruiz , M. Zychowicz , H. Rauscher , L. Ceriotti , F. Rossi , P. Colpo , K. Domanska-Janik , S. Coecke
• Języki publikacji: EN

Abstrakty

EN

Bio-functionalized surfaces were prepared to study the adherence and differentiation capacity of neural stem cells derived from human umbilical cord blood (HUCB-NSC). Cell growth platforms containing arranged arrays of adhesive molecules were created by microcontact printing on a biologically inert surface. Biomolecules used to prepare microarray platforms included the extracellular matrix protein fibronectin and the polyaminoacid poly-L-lysine. HUCB-NSC plated on microplatforms at various serum conditions showed serum and molecule type dependent capacity for adhesion and differentiation. Poly-L-lysine allowed the maintenance of stem-like non differentiated cells attached to the surface, whereas fibronectin promoted spreading and neural commitment. Serum deprivation did not influence the attachment of HUCB-NSC to fibronectin, but significantly enhanced the attachment to poly-L-lysine and promoted dBcAMP induced neuronal differentiation. A bio-pattern of squares with interconnecting lines was used to guide neuronal differentiation by directing cell protrusion outgrowth. Tailoring the geometry of the bio-pattern enabled directing and monitoring of the neural stem cells. development in the large scale multiparameter biotests.

Słowa kluczowe

EN

CORD BLOOD; MICROPATTERNING; NEURAL STEM CELL

• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 1
• Strony: 24-36

Twórcy

autor

L. Buzanska

autor

A. Ruiz

autor

M. Zychowicz

autor

H. Rauscher

autor

L. Ceriotti

autor

F. Rossi

autor

P. Colpo

autor

K. Domanska-Janik

autor

S. Coecke

• Typ dokumentu: ARTICLE
• Bibliografia: Leonora Buzanska, NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland