Metody wykrywania mikroorganizmów patogennych w żywności

• Autorzy: Daniela Gwiazdowska , Marian Filipiak

Warianty tytułu

Methods of Detecting Pathogenic Microorganisms in Food

• Języki publikacji: PL

Abstrakty

Skażenie żywności przez chorobotwórcze patogeny i toksyny stanowi jeden z najważniejszych problemów zdrowotnych współczesnego świata. Zjawisko to dotyczy zarówno krajów wysoko uprzemysłowionych, jak i słabo rozwiniętych. Epidemiolodzy szacują, że choroby wywołane przez mikroorganizmy patogenne są przyczyną około 40% całkowitej liczby zgonów na świecie. Lista mikroorganizmów patogennych obejmuje bakterie, pasożyty i wirusy, przy czym niemal 75% wszystkich zatruć spowodowanych jest przez bakterie. (fragment tekstu)

EN

There are millions of food poisoning cases in the world every year and a significant part of them ends in death. This is a problem for both the poorly and highly developed countries. Most frequently the poisoning is caused by such bacteria as Campylobacter, Salmonella, Clostridium and Staphylococcus, but many illnesses are of unknown etiology. In order to ensure food safety it is necessary to detect quickly and precisely the microorganisms responsible for food poisoning. This can be done thanks to new analytical techniques which have dynamically developed over the last few years. The paper is a review of the currently applied methods to detect and identify pathogenic microorganisms in food. The authors present a series of hygiene related technological procedures, such as: bioluminescence measurement of ATP, measurement of impedance, microscopic methods or membrane filtration. In order to ensure fast selective detection and identification of pathogenic organisms, a wider and wider use is made of polymerization chain reaction (PCR), genetic probes, chromogen and serologic tests as well as biosensors. However, conventional microbiological methods which, in contrast to majority of fast methods, render both quantitative and qualitative results, are still treated as reference methods. (original abstract)

Słowa kluczowe

PL

Toksykologia żywności; Badanie żywności; Bezpieczeństwo zdrowotne żywności; Żywność; Mikrobiologia

EN

Food toxicology; Food research; Food health safety; Food; Microbiology

• Czasopismo: Zeszyty Naukowe / Akademia Ekonomiczna w Poznaniu
• Rocznik: 2006
• Numer: nr 73
• Strony: 25-39

Twórcy

autor

Daniela Gwiazdowska

autor

Marian Filipiak

Bibliografia

• Adamczak M., Bednarski W., Kowalska A., Szybkie testy biologiczne szansą poprawy i standaryzacji jakości żywności, Przemysł Spożywczy 6, 1999, s. 36-39.
• Allmann M., Hofelein C., Koppel E., Luthy J., Meyer R., Niederhauser C., Wegmuller B., Candrian U., Polymerase chain reaction (PCR) for the detection of pathogenic microorganisms in bacteriological monitoring of dairy products, Res. Microbiol. 146, 1995, s. 85-97.
• Bautista D.A., Clarke R., Vaillancourt J.P., Renwick S., Griffith M.W., Adenosine triphosphate bioluminesce as a method to determine microbial levels in scald and chill tanks at a poultry abattoir, Poultry Sci. 73, 1994, s. 1673-1678.
• Bautista D.A., Mclntyre L., Laleye L., Griffith M.W., The application of ATP bioluminescense for the assessment of milk quality and factory hygiene, J. Rapid Methods Automat. Microbiol. 1, 1992, s. 179-193.
• Betzl D., Ludwig W., Schleifer K.H., Identification of lactococci and enterococci by colony hybridisation with 23S rRNA - targeted oligonuclease probes, Appl. Environ. Microbiol. 52, 1990, s. 1190-1194.
• Bishop B.F., Lorbert S.J., The needs for sensors in bacterial and yeast fermentation, Bioprocess. Technol. 6, 1990, s. 1-16.
• Blankenfeld-Enkvist G. von, Brannback M., Technological trends and needs in food diagnostics, Tekes, National Technology Agency, Technology Review 132, 2002.
• Bulte M., Dot blot - Verfahren mit Digoxigenin - markierten Gensonden zum Nachwels von verotoxinogen E.coli - Stammen (VTEC) in Verdunnungskulturen und Lebensmittelproben, Archiv fur Lebensmittelhygiene 42, 1991, s. 77-100.
• Che Y.H., Yang Z.P., Li Y.B., Paul D., Slavik M., Rapid detection of Salmonella typhimurium using an immunoelectrochemical method coupled with immunomagnetic Separation, J. Rapid Methods Automat. Microbiol. 7 (1), 1999, s. 47-59.
• Doyle M.P., Reducing foodborne disease: what are priorities?, Nutrition 16 (7/8), 2000.
• Fach P., Hauser D., Guillou J.P., Popoff M.R., Polymerase chain reaction for the rapid identification of Clostridium botulinum type A strains and detection in food samples, J. Appl. Bacteriol. 61, 1993, s. 389-392.
• Flint S.H., Brooks J.D., Bremer P.J., Use of the Malthus conductance growth analyser to determine numbers of thermophilic streptococci on stainless steel, J. Appl. Microbiol. 1997.
• Ge B., Zhao S., Hall R., Meng J., A PCR-ELISA for detecting Shiga toxin - producing Escherichia coli, Microbes and Infection 4, 2002, s. 285-290.
• Griffith M.W., Rapid estimation of microbial numbers in dairy products using ATP technology, w: Physical methods for microorganisms detection, ed. W.H., Nelson, CRC Press, Boca Raton 1991, s. 29-62.
• Griffith M.W., The role of ATP bioluminescence in food industry: New light on old problem, Food Technol. 6, 1996, s. 62-71.
• Herman L.M.F., De Ridder H., Cheese components reduce the sensitivity of detection of Listeria monocytogenes by the polymerase chain reaction, Neth. Milk Dairy J. 47, 1993, s. 23-29.
• Jones D.D., Bej A.K., Detection of foodborne microbial pathogens using polymerase chain reaction methods, w: PCR technology: Current innovations, eds H.G. Griffin, A.M. Griffin, CRC Press Inc., London 1994, s. 341-365.
• Josephson K.L., Gerba C.P., Pepper I.L., Polymerase chain reaction of nonviable bacterial pathogens, Appl. Environ. Microbiol. 59, 1993, s. 3513-3515.
• Kircher D., Buelte M., Reuter G., Suitability of a bioluminescence method for examination, cleaning and disinfection in food processing, Fleischwirtschaft 76, 1996, s. 897-903.
• Klegerman M.E., Automated microbial identification and quantification, w: Technologies for the 2000s, ed. W.P. Olson, Interpharm Press, Buffalo Grove 1996, s. 259-279.
• Koch W.H., Payne W.L., Wentz B.A., Cebula T.A., Rapid polymerase chain reaction method for detection of Vibrio cholerae in foods, Appl. Environ. Microbiol. 59, 1993, s. 556-560.
• Kroll R., The direct immunofluorescent filter technique (DIFT) w: Methods in molecular biology, vol. 46: Diagnostic bacteriology protocols, eds J. Howard, D.M. Whitcombe, 1995, s. 107-112.
• Kyriakides A.L., Patel P.D., Luminescence techniques for microbial analysis of food, w: Rapid analysis techniques for microbiology, ed. P. Patel, Blackie Academic and Professional Press, Glasgow, UK, 1994, s. 196-231.
• Leonard P., Hearty S., Brennan J., Dunne L., Quinn J., Chakraborty T., O'Kennedy R., Advances in biosensors for detection of pathogens in food and water, Enz. Microbial Technol. 32, 2003, s. 3-13.
• Michalski M., Zastosowanie HY-LITE w monitoringu w ramach systemu HACCP, Przemysł Spożywczy 6, 1999, s. 23-25.
• Olsen J.E., DNA - based methods for detection of foodborne bacterial pathogens, Food Res. Int. 33 (3-4), 2000, s. 257-266.
• Olsen J.E., Aabo S., Nielsen E.O., Nielsen B.B., Isolation of a Salmonella specific DNA hybridization probe, APMIS 99, 1991, s. 114-120.
• Olsen O., Rapid food microbiolgy: Application of bioluminescence in the dairy and food industry - a review, w: Physical methods for microorganisms detection, ed. W.H. Nelson, CRC Press, Boca Raton 1991, s. 68-80.
• Perez F.G., Mascini M., Tothill I.E., Turner A.P., Immunomagnetic separation with mediated flow injection analysis amperometric detection of viable Escherichia coli 0157, Anal. Chem. 70 (11), 1998, s. 2380-2386.
• Pettipher G.L., Mansell R., McKinnon C.H., Cousins CM., Rapid membrane filtration - epifluorescent microscopy technique for direct enumeration of bacteria in raw milk, Appl. Environ. Microbiol. 39 (2), 1980, s. 423-429.
• Pytlik G., Nierychlewski B., Mueller P., Zastosowanie technik filtracji membranowej w kontroli mikrobiologicznej wody, Aparatura Badawcza i Dydaktyczna 8 (2), 2003, s. 205-209.
• Reybroeck W., Schramm E., Improved filtration method to assess bacteriological quality of raw milk based on bioluminescence of adenosine triphosphate, Neth Milk Dairy J. 49, 1995, s. 1-14.
• Rossen L., Holmstrom K., Olsen J.E., Rasmussen O.F., A rapid polymerase chain reaction (PCR) - based assay for the identification of Listeria monocytogenes in food samples, Int. J. Food Microbiol. 14, 1991, s. 145-152.
• Rossen L., Norskov P., Holmstrom K., Rasmussen O.F., Inhibition of PRC by components of food samples, microbial diagnostic assays and DNA - extraction solutions, Int. J. Food Microbiol. 17, 1992, s. 37-45.
• Rozporządzenie Ministra Środowiska z dnia 27 listopada 2002 r., DzU z 2002 r., nr 204, poz. 1728.
• Ryan R.A., Bioluminescence testing of aseptically packaged fruit juice, Milk Ind. Int. 98, 1996, s. 13-15.
• Salama M.S., Sandine W.E., Giovannoni S.J., Isolation of Lactococcus lactis subsp. cremoris from nature by colony hybridization with rRNA probes, Appl. Environ. Microbiol. 59, 1993, s. 3941-3945.
• Sala-Newby G.B., Goodfield G., Johnson I.R., Massey P.G., Stadford D.A., Hampton A.N., Campbell A.K., Ultra-sensitive detection of microbes by ATP analysis, w: ATP luminescence rapid methods in microbiology, eds P.E. Stanley, B.J. McCarthy, R. Smither, Blackwell Scientific Publications, Oxford 1989, s. 261-270.
• Scheu P.M., Berghof K., Stahl U., Detection of pathogenic and spoilage microorganisms in food with the polymerase chain reaction, Food Microbiol. 15, 1998, s. 13-31.
• Schmidthuber S., Ludwig W., Schleifer K.H., Construction of a DNA probe for the specific identification of Streptococcus oralis, J. Clin. Microbiol. 26, 1988, s. 1042-1044.
• Scholl D.R., Kaufmann C., Jollick J.D., York C., Goodrum G.R., Charche P., Clinical application of novel sample processing technology for the identification of Salmonelle by using DNA probes, J. Clin. Microbiol. 28, 1990, s. 237-241.
• Simpson W.J., 'Instant' assessment of brewery hygiene using ATP bioluminescence, Brewers' Guardian 10, 1989, s. 20-22.
• Smith T.J., O'Connor L., Glennon M., Maher M., Molecular diagnostic in food safety: Rapid detection of foodborne pathogens, Irieh J. Agric. Food Res. 39 (2), 2000, s. 309-319.
• Tortorello M.L., Gendel S.M., Fluorescent antibodies applied to direct epifluorescent filter technique for microscopic enumeration of Escherichia coli 0157:H7 in milk and juice, J. Food Protect. 56, 1993, s. 672-677.
• Tortorello M.L., Stewart D.S., Antibody - direct epifluorescent filter technique for rapid, direct enumeration of Escherichia coli 0157:117 in beef, Appl. Environ. Microbiol. 60, 1994, s. 3553-3559.
• Ur A., Brown D.F., Impedance monitoring of bacterial activity, J. Med. Microbiol. 8(1), 1975, s. 19-28.
• Velasco-Garcia M.N., Mottram T., Biosensor technology addressing agricultural problems, Biosystems Engineering 84 (1), 2003, s. 1-12.
• Wang H., Sharpe A.N., An immuno-capturing and concentrating procedure for Escherichia coli 0157:117 and its detection by epifluorescent microscopy, Food Microbiol. 15, 1998, s. 559-565.
• Way J.S., Josephson K.L., Pillai S.D., Abbaszadegan M., Gerba C.P., Pepper I.L., Specific detection of Salmonella spp. by multiplex polymerase chain reaction, Appl. Environ. Microbiol. 59, 1993, s. 1473-1479.
• Werlein H.D., Fricke R., ATP bioluminescence for rapid determination of the quality of poultry meat, Archiv fur Geflugelkunde 60, 1996, s. 212-217.
• Ye J., Letcher S.V., Rand A.G., Piezoelectric biosensor for detection of Salmonella typhimurium, J. Food Sci. 62 (5), 1997, s. 1067-1071.
• Zakrzewska-Czerwińska J., Sondy molekularne w diagnostyce mikrobiologicznej, Biotechnologia 4 (59), 2002, s. 79-89.
• Zaremba M.L., Borowski J., Podstawy mikrobiologii lekarskiej, PZWL, Warszawa 1994, s. 47-49.