Competitiveness of Rhizobium leguminosarum bv. trifolii strains in mixed inoculation of clover (Trifolium pratense)

• Autorzy: Wielbo J. , Marek-Kozaczuk M. , Kidaj D. , Skorupska A.
• Języki publikacji: EN

Abstrakty

EN

Rhizobium leguminosarum bv. trifolii (Rlt) establishes beneficial root nodule symbiosis with clover. Twenty Rlt strains differentially marked with antibiotic-resistance markers were investigated in terms of their competitiveness and plant growth promotion in mixed inoculation of clover in laboratory experiments. The results showed that the studied strains essentially differed in competition ability. These differences seem not to be dependent on bacterial multiplication in the vicinity of roots, but rather on complex physiological traits that affect competitiveness. The most remarkable result of this study is that almost half of the total number of the sampled nodules was colonized by more than one strain. The data suggest that multi-strain model of nodule colonization is common in Rhizobium-legume symbiosis and reflects the diversity of rhizobial population living in the rhizosphere.

Słowa kluczowe

EN

biological nitrogen fixation; Rhizobium leguminosarum bv.trifolii; competitiveness; mixed inoculation; rhizobium; clover; Trifolium pratense; root nodule symbiosis; Rlt strain; plant growth promotion; rhizosphere

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2011
• Tom: 60
• Numer: 1
• Opis fizyczny: p.43-49,fig.,ref.

Twórcy

autor

Wielbo J.

• Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Akademicka 19 st., 20-033 Lublin, Poland

autor

Marek-Kozaczuk M.

autor

Kidaj D.

autor

Skorupska A.

Bibliografia

• Bromfield E.S.P. 1984. Variation in preference for Rhizobium meliloti within and between Medicago sativa cultivars grown in soil. Appl. Environ. Microbiol. 48: 1231-1236.
• Cresswell A., L. Skot and A.R. Cookson. 1994. The construction, detection and use of bioluminescent Rhizobium leguminosarum biovar trifolii strains. J. Appl. Bacteriol. 77: 656-665.
• Depret G. and G. Laguerre. 2008. Plant phenology and genetic variability in root and nodule development strongly influence genetic structuring of Rhizobium leguminosarum biovar viciae populations nodulating pea. New Phytol. 179: 224-235.
• Dowling D.N. and W.J. Broughton. 1986. Competition for nodulation of legumes. Annu. Rev. Microbiol. 40: 131-157.
• Duodu S., C. Brophy, J. Connolly and M.M. Svenning. 2009. Competitiveness of native Rhizobium leguminosarum bv. trifolii strain for nodule occupancy is manifested during infection. Plant Soil 318: 117-126.
• Duodu S., T.V. Bhuvaneswari, J. Gudmundsson and M.M. Svenning. 2005. Symbiotic and saprophytic survival of three unmarked Rhizobium leguminosarum biovar trifolii strains introduced into the field. Environ. Microbiol. 7: 1049-1058.
• Gage D.J. 2004. Infection and invasion of roots by symbiotic nitrogen-fixing rhizobia during nodulation of temperate legumes. Microbiol. Mol. Biol. Rev. 68: 280-300.
• Jones K.M., H. Kobayashi, B.W. Davies, M.E. Taga and G.C. Walker. 2007. How symbionts invade plants: the Sinorhizobium-Medicago model. Nature 5: 619-633.
• Kiers E.T., R.A. Rousseau and R.F.Denison. 2006. Measured sanctions: legume hosts detect quantitative variation in rhizobium cooperation and punish accordingly. Evol. Ecol. Res. 8: 1077-1086.
• Lakzian A., P. Murphy, A. Turner, J.L. Beynon and K.E. Giller. 2002. Rhizobium leguminosarum bv. viciae populations in soils with increasing heavy metal contamination: abundance, plasmid profiles, diversity and metal tolerance. Soil Biol. Biochem. 34: 519-529.
• Lopez-Garcia S.L., T.E.E. Vazquez, G. Favelukes and A.L. Lodeiro. 2002. Rhizobial position as a main determinant in the problem of competition for nodulation in soybean. Environ. Microbiol. 4: 216-224.
• Mabood F., W.J. Jung and D.L. Smith. 2008. Signal in the underground: microbial signaling and plant productivity. In: Nautiyal C.S., P.E. Dion and V.L. Chopra (eds.). Molecular mechanisms of plant and microbe coexistence. Springer-Verlag, Berlin, Heidelberg.
• Maier R.J. and E.W. Triplett. 1996. Toward more productive, efficient, and competitive nitrogen-fixing symbiotic bacteria. Crit. Rev. Plant Sci. 15: 191-234.
• Maj D., J. Wielbo, M. Marek-Kozaczuk and A. Skorupska. 2009. Pretreatment of clover seeds with Nod factors improves growth and nodulation of Trifolium pratense. J. Chem. Ecol. 35: 479-487.
• Maj D., J. Wielbo, M. Marek-Kozaczuk and A. Skorupska. 2010. Response to flavonoids as a factor influencing competitiveness and symbiotic activity of Rhizobium leguminosarum. Microbiol. Res. 165: 50-60.
• Masson-Boivin C., E. Giraud, X. Perret and J. Batut. 2009. Establishing nitrogen-fixing symbiosis with legumes: how many rhizobium recipes? Trends Microbiol. 17: 458-466.
• Mellor H.Y., A.R. Glenn and M.J. Dilworth. 1987. Symbiotic and competitive properties of motility mutants of Rhizobium trifolii TA1. Arch. Microbiol. 148: 34-39.
• Peoples M.B, J.K. Ladha and D.F. Herridge. 1995. Enhancing legume N₂ fixation through plant and soil management. Plant Soil 174: 83-101.
• Perret, X., C. Staehelin and W.J. Broughton. 2000. Molecular basis of symbiotic promiscuity. Microbiol. Mol. Biol. Rev. 64: 180-201.
• Ramos M.G., M.A. Villatoro, S. Urquiaga, B.J. Alves and R.M. Boddey. 2001. Quantification of the contribution of biological nitrogen fixation. J. Biotechnol. 91: 105-115.
• Rangin, C., B. Brunel, J.-C. Cleyet-Marel and G. Bena. 2008. Effects of Medicago truncatula Genetic Diversity, Rhizobial Competition, and Strain Effectiveness on the Diversity of a Natural Sinorhizobium Species Community. Appl. Environ. Microbiol. 74: 5653-5661.
• Sachs J.L., S.W. Kembel, A.H. Lau and E.L. Simms. 2009. In situ phylogenic and diversity of wild Bradyrhizobium communities. Appl. Environ. Microbiol. 75: 4727-4735.
• Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: a Laboratory Manual. 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
• Sessitsch A., G. Hardarson, W.M. de Vos and K.J. Wilson. 1998. Use of marker genes for competition studies of Rhizobium. Plant Soil 204: 35-45.
• Sharma P.K., R.C. Anand and K. Lakshminarayana. 1991. Construction of Tn5 tagged mutants of Rhizobium spp. (Cicer) for ecological studies. Soil Biol. Biochem. 23: 881-885.
• Silva C., F.L. Kan and E. Martínez-Romero. 2007. Population genetic structure of Sinorhizobium meliloti and S. medicae isolated from nodules of Medicago spp. in Mexico. FEMS Microbiol. Ecol. 60: 477-489.
• Spaink H.P. 2000. Root nodulation and infection factors produced by rhizobial bacteria. Annu. Rev. Microbiol. 54: 25-288.
• Streeter J.G. 1994. Failure of inoculant rhizobia to overcome the dominance of indigenous strains for nodule formation. Can. J. Microbiol. 40: 513-522.
• Stuurman N., C. Pacios Bras, H.R.M. Schlaman, A.H.M. Wijfjes, G. Bloemberg and H.P. Spaink. 2000. Use of green fluorescent protein color variants expressed on stable broad-hostrange vectors to visualize rhizobia interacting with plants. Mol. Plant-Microbe Interact. 13: 1163-11699.
• Svenning M.M., J. Gudmundsson, I.L. Fagerli and P. Leinonen. 2001. Competition for nodule occupancy between introduced strains of Rhizobium leguminosarum bv. trifolii and its influence on plant production. Ann. Bot. 88: 781-787.
• Timmers A.C., E. Soupène, M.C. Auriac, F. de Billy, J. Vasse, P. Boistard and G. Truchet. 2000. Saprophytic intracellular rhizobia in alfalfa nodules. Mol. Plant-Microbe Interact. 13: 1204-1213.
• Vasse J., F. de Billy, S. Camut and G. Truchet. 1990. Correlation between ultrastructural differentiation of bacteroids and nitrogen fixation in alfalfa nodules. J. Bacteriol. 172: 4295-4306.
• Vincent J.M. 1970. A manual for the practical study of root nodule bacteria. International biological program handbook no.15. Blackwell Scientific Publications Ltd, Oxford, UK.
• Wielbo J. and A. Skorupska. 2001. Construction of improved vectors and cassettes containing gusA and antibiotic resistance genes for studies of transcriptional activity and bacterial localization. J. Microbiol. Methods 45: 197-205.
• Wielbo J., M. Marek-Kozaczuk, A. Kubik-Komar and A. Skorupska. 2007. Increased metabolic potential of Rhizobium spp. is associated with bacterial competitiveness. Can. J. Microbiol. 53: 957-967.
• Wielbo J., Marek-Kozaczuk M., Mazur A., Kubik-Komar A. and A. Skorupska. 2010. Genetic and metabolic divergence within a Rhizobium leguminosarum bv. trifolii population recovered from clover nodules. Appl. Environ. Microbiol. 76: 4593-4600.
• Wilson R.A., B.A. Handley and J.E. Beringer. 1998. Bacteriocin production and resistance in a field population of Rhizobium leguminosarum bv. viciae. Soil Biol. Biochem. 30: 413-417.