Apoptosis induction of human peripheral blood lymphocytes by naturally occurring coumarins

• Autorzy: Kocki J. , Bogucka-Kocka A.
• Języki publikacji: EN

Abstrakty

EN

Our studies show the influence of coumarins: xanthotoxin and o,o-dimethyl-fraxetin on apoptosis in human peripheral blood lymphocytes in vitro. Lymphocytes (derived by ethical protocol) were cultured for 72 h in vivo. The cell cultures were stimulated with xanthotoxin or o,o-dimethyl-fraxetin in various concentrations. Apoptotic cells (compaction and margination of nuclear chromatin, cytoplasmic condensation, membrane blebbing and cell shrinkage) were observed in BX41 fluorescent Olympus microscope and recorded by MultiScan software. We observed the highest induction of apoptosis in cells stimulated with xanthotoxin in doses ≥50 μM in 48-hrs-cultures.

Słowa kluczowe

EN

apoptosis induction; man; peripheral blood; lymphocyte; coumarin; xanthotoxin; o,o-dimethyl-fraxetin; apoptosis; cell culture

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2004
• Tom: 13
• Numer: Suppl.2, Part 1
• Opis fizyczny: p.221-224,fig.,ref.

Twórcy

autor

Kocki J.

• Department of Medical Genetics, Medical University of Lublin, 11 Radziwillowska Str., 20-950 Lublin, Poland

autor

Bogucka-Kocka A.