Effect of partial and complete blockade of vanilloid (TRPV1-6) and ankyrin (TRPA1) transient receptor potential ion channels on urinary bladder motor activity in an experimental hyperosmolar overactive bladder rat model

• Autorzy: Juszczak K. , Ziomber A. , Thor P.J.
• Języki publikacji: EN

Abstrakty

EN

The study investigated the mechanisms through which the hyperosmolarity might induce detrusor overactivity (DO). We compared the bladder activity in response to partial and complete blockade of TRPV1-6 and TRPA1 receptors. Experiments were performed on 42 rats. DO was induced by using hyperosmolar saline. All animals were randomly divided into six groups. The measurements represent the average of five bladder micturition cycles. Hyperosmolar saline induced DO. The complete blockade of TRPV1-6 and TRPA1 prevented DO. The partial blockade of TRPV1 didn't prevented DO. In the voiding phase periodical bladder contractions complexes occurred leading to slow urine flow due to bladder distension. Ruthenium red and capsaicin resulted in complete disorganisation of detrusor muscle contractility impairing urine voiding and leading to constantly lasting urine retention in healthy rats. Conclusions: hyperosmolar-induced DO is mediated by TRPV and TRPA1 channels; the hyperosmolar stimuli of urinary bladder might be transmitted mostly via ruthenium red sensitivity pathway.

Słowa kluczowe

EN

bladder distension; capsaicin; detrusor overactivity; hyperosmolarity; motor activity; rat; ruthenium red; transient receptor potential ankyrin 1; transient receptor potential vanilloid type 1; urinary bladder; urine retention

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2011
• Tom: 62
• Numer: 3
• Opis fizyczny: p.321-326,fig.,ref.

Twórcy

autor

Juszczak K.

• Department of Pathophysiology, Jagiellonian University, Medical College. 18 Czysta Street, 31-121 Krakow, Poland

autor

Ziomber A.

autor

Thor P.J.