Warianty tytułu
Application of PCR reaction for identification of Plasmodiophora brassicae Wor. (clubroot) and pathotype P1
Języki publikacji
Abstrakty
Clubroot disease is one of the most serious diseases of oilseed rape and other Brassicaceae species. The disease causual agent is an obligatory parasite – Plasmodiophora brassicae. Typical symptoms of the infection are specific round tumors on roots. Several pathotypes of P. brassicae are known. It is possible to identify the pathotypes using a root hair test or by PCR. The aim of the study was to check the possibility of using PCR to identify oilseed rape infection by P. brassicae and its P1 pathotype. Plant samples with clubroot sympthoms were colleted from Poland in 2008–2009. PCR primers specific to P. brassicae and pathotype P1 were used. Infection of 44 tested plants by P. brassicae and the presence of P1 pathotype in six samples was confirmed PCR reaction. The occurance of clubroot disease was also recorded on Shepherd’s Purse, which may be considered as a potential inoculum source of this pathogen.
Słowa kluczowe
Wydawca
Czasopismo
Rocznik
Tom
Numer
Opis fizyczny
s.705-708,tab.,bibliogr.
Twórcy
autor
- Instytut Ochrony Roślin – Państwowy Instytut Badawczy, ul.Władysława Węgorka 20, 60-318 Poznań
autor
autor
autor
Bibliografia
- Fiedorow Z., Gołębniak B., Weber Z. 2008. Choroby Roślin Rolniczych. AR, Poznań, 208 ss.
- Manzanares-Dauleux M.J., Barret P., Thomas G. 2000. Development of a pathotype specific SCAR marker in Plasmodiophora brassicae. Eur. J. Plant Pathol. 106: 781–787.
- Robak J. 1991. Zmienność patotypów Plasmodiophora brassicae Wor. występujących w Polsce i ich patogeniczność w stosunku do odmian i linii hodowlanych Brassicae oleracea. Rozpr. hab. Instytut Warzywnictwa, 61 ss.
- Staniaszek M., Robak J., Marczewski W. 2001. Detection of Plasmodiophora brassicae Wor. by bioassay and nested PCR methods. Veg. Crops Res. Bull. 54 (1): 131–137.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.dl-catalog-1ef26d26-d446-4c75-9c19-6337ad5865c3