Screening procedure for simultaneous determination of azaperone, carazolol, and chlorpromazine in animal urine

• Autorzy: Sell B. , Posyniak A. , Grylik M.
• Języki publikacji: EN

Abstrakty

EN

A liquid chromatographic method coupled with UV-Vis and fluorescence detectors for the determination of azaperone, carazolol, and chlorpromazine residues in urine has been described. Analytes were isolated from biological samples with acetonitrvle. The obtained extracts were applied to solid phase extraction Strata X-C 33 µn Polimeric Strong Cation columns. The analytes were eluted with alkalised acetonitrvle. The whole procedure was validated according to European Decision 657/2002/EC. The detection limits were established at the level 2 µg/L for Carazolol and 5 µg/L for azaperone and chlorpromazine. Detection capabilities for carazolol, azaperone, and chlorpromazine were 8.62, 18.9, and 6.53 µg/L, respectively. Recoveries were above 90%.

Słowa kluczowe

EN

screening procedure; simultaneous determination; azaperone; carazolol; chlorpromazine; animal urine; urine; determination; liquid chromatography; neuroleptic

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2011
• Tom: 55
• Numer: 3
• Opis fizyczny: p.513-517,fig.,ref.

Twórcy

autor

Sell B.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Posyniak A.

autor

Grylik M.

Bibliografia

• 1. Aoki Y., Hakamata H., Igarashi Y., Uchida K., Kobayashi H., Hirayama N., Kotani A., Kusu F.: Simultaneous determination of azaperone and azaperol in animal tissues by HPLC with confirmation by electrospray ionization mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 2009, 877, 166-172.
• 2. Bostedt H., Rudloff P.R.: Prophylactic administration of the ß-blocker carazolol to influence the duration of parturition in sows. Theriogenology 1983 , 20, 191-196.
• 3. Cerkvenik-Flajs V.: Determination of residues of azaperone in the kidneys by liquid chromatography with fluorescence detection. Anal Chim Acta 2007, 586, 374- 382.
• 4. Commission Regulation (EU) 37/2010 of 22 December 2009 on pharmacologically active substance and their classification regarding maximum residue limits in foodstuffs of animal origin. O. J. E. U. L15/1, 20.1.2010.
• 5. Delahaut P., Brasseur P., Dubois M.: Multiresidue method for the detection of tranquillizers, xylazine and a ß-blocker in animal production by liquid chromatography-tandem mass spectrometry. J Chromatogr A 2004, 1054, 373-387.
• 6. Fluchard D., Kiebooms S., Dubois M., Delahaut P.: Determination of a method for detecting and quantifying azaperone, azaperol and carazolol in pig tissues by liquid chromatography-tandem mass spectrometry. J Chromatogr B Biomed Sci Appl 2000, 744, 139-147.
• 7. Gocke E.: Review of the genotoxic properties of chlorpromazine and related phenothiazines. Mut Res/Rev Gen Toxicol 1996, 366, 9-21.
• 8. Josefsson M., Kronstrand R., Andersson J., Roman M.: Evaluation of electrospray ionisation liquid chromatography-tandem mass spectrometry for rational determination of a number of neuroleptics and their major metabolites in human body fluids and tissues. J Chromatogr B Analyt Technol Biomed Life Sci 2003, 789, 151-167.
• 9. Kaufmann A., Butcher P., Maden K., Widmer M.: Ultraperformance liquid chromatography coupled to time of flight mass spectrometry (UPLC-TOF): A novel tool for multiresidue screening of veterinary drugs in urine. Anal Chim Acta 2007, 586, 13-21.
• 10. Keukens H. J., Aerts M. M. L.: Determination of residues of carazolol and a number of tranquilizers in swine kidney by high-performance liquid chromatography with ultraviolet and fluorescence detection. J Chromatogr 1989, 464, 149-161.
• 11. Kozuh Erzen N.: Analitska metoda za ugotavljanje zaostankov klopromazina v misicnem tkivu in urinu klavnih zivali. Slov Vet Res 2001, 38, 305-312.
• 12. Mitrowska K., Posyniak A., Zmudzki J.: Rapid method for the determination of tranquilizers and a beta-blocker in porcine and bovine kidney by liquid chromatography with tandem mass spectrometry. Anal Chim Acta 2009, 637, 185-192.
• 13. Rose M. D., Shearer G.: Determination of tranquilizers and carazolol residues in animal tissue using high- performance liquid chromatography with electrochemical detection. J Chromatogr A 1992, 624, 471-477.
• 14. Quintana M.C., Blanco M. H., Lacal J., Hernandez L.: Analysis of promazines in bovine livers by high performance liquid chromatography with ultraviolet and fluorimetric detection. Talanta 2003, 59, 417-422.
• 15. Van Ginkel L.A., Scwillens P. L. W. J., Olling M.: Liquid chromatographic method with on-line UV spectrum identification and off-line thin-layer chromatographic confirmation for the detection of tranquilizers and carazolol in pig kidneys. Anal Chim Acta 1989, 225, 137- 146.
• 16. Zhang J., Shao B., Yin J., Wu Y., Duan H.: Simultaneous detection of residues of ß-adrenergic receptor blockers and sedatives in animal tissues by high-performance liquid chromatography/ tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 2009, 877, 1915-1922.