Salivary hexosaminidase B in children with type 1 diabetes

• Autorzy: Zalewska-Szajda, B , Chojnowska, S , Waszkiewicz, N , Gościk, E , Łebkowska, U , Kępka, A , Bossowski, A , Kuźmiuk, A , Mierzyńska, K , Szajda, SD , Ładny, JR

Abstrakty

EN

Introduction: Children type 1 diabetes is accompanied by inflammation and microangiopathy pro-ceeded with increased degradation of tissues. Nacetyl-β-hexosaminidase (HEX) is the most active of exoglycosidases degrading oligo-saccharide chains of glycoconjugates (glycoproteins, glycolipids and proteoglycans). Purpose: To evaluate the hexosaminidase B (HEX B) activity in the saliva of children with type 1 diabetes. Materials and methods: The study was performed in 35 children with type 1 diabetes and 20 healthy children. Salivary HEX B activity was determined by the colorimetric, and protein by bicinchoninic acid methods. The HEX B activity concentration was expressed in pKat/mL and specific activity in pKat/µg of protein. Results: A significant increase in the concentration and the specific activity of HEX B in the saliva of children with type 1 diabetes, as compared to healthy children, was found. Conclusion: Children suffering from type 1 diabetes have increased catabolism of salivary gly-coconjugates by HEX B, which potentially may be useful in the diagnosis of type 1 diabetes in children.

Słowa kluczowe

EN

hexosaminidase isoenzyme B (HEX B); type 1 diabetes; children; saliva

• Wydawca: Uniwersytet Medyczny w Białymstoku
• Czasopismo: Progress in Health Sciences
• Rocznik: 2012
• Tom: 2
• Numer: 2
• Strony: null-140

Twórcy

autor

Zalewska-Szajda, B

autor

Chojnowska, S

autor

Waszkiewicz, N

autor

Gościk, E

autor

Łebkowska, U

autor

Kępka, A

autor

Bossowski, A

autor

Kuźmiuk, A

autor

Mierzyńska, K

autor

Szajda, SD

autor

Ładny, JR

Identyfikatory

DOI

143