Inhibition of 12/15-LOX activity and ceramide pattern in the murine brain

• Autorzy: Fiedorowicz, A , Car, H , Prokopiuk, S , Sacharzewska, E , Żendzian-Piotrowska, M , Kowal, K

Abstrakty

EN

Purpose: The 12/15-lipoxygenase (12/15-LOX) activity and the ceramide content are often elevated in neurodegenerative disorders; however their relationship in the brain is not established. To verify whether blocking of 12/15-LOX activity has an impact on ceramide pattern in the brain we inhibited the 12/15-LOX expression by administration of baicalein in mice. Materials and methods: The ceramides containing fatty acid methyl esters were analyzed using gas–liquid chromatography (GLC) technique. Results: The total ceramide content increased in baicalein-treated animals comparing to controls and the levels of most ceramide-fatty acids of SAFA, MUFA and PUFA tended to increase in relation to control. Baicalein treatment up-regulated significantly only the ceramide-lignoceric fatty acid in relation to controls. Conclusion: We have shown in this study that 12/15-LOX inhibition slightly alters the pattern of ceramides in the mouse brain.

Słowa kluczowe

EN

12/15-lipoxygenase; ceramide; baicalein; mice

• Wydawca: Uniwersytet Medyczny w Białymstoku
• Czasopismo: Progress in Health Sciences
• Rocznik: 2013
• Tom: 3
• Numer: 1
• Strony: 33-38

Twórcy

autor

Fiedorowicz, A

autor

Car, H

autor

Prokopiuk, S

autor

Sacharzewska, E

autor

Żendzian-Piotrowska, M

autor

Kowal, K