Gradient elution LC-MS determination of dasatinib in rat plasma and its pharmacokinetic study

• Autorzy: Wen, C. , Zhang, Q. , He, Y. , Deng, M. , Wang, X. , Ma, J.
• Języki publikacji: EN

Abstrakty

EN

A sensitive and simple liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) method for determination of dasatinib in rat plasma using one-step protein precipitation was developed. After addition of carbamazepine as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographic separation was achieved on an SB-C18 (2.1 mm × 150 mm, 5 μm) column with methanol-0.1% formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selective ion monitoring (SIM) mode was used to quantification using target fragment ions m/z 488.2 for dasatinib and m/z 338.7 for the IS. Calibration plots were linear over the range of 10–1000 ng mL−1 for dasatinib in rat plasma. Lower limit of quantification (LLOQ) for dasatinib was 10 ng mL−1. Mean recovery of dasatinib from plasma was in the range 82.2%–93.6%. Relative standard deviation (RSD) of intra-day and inter-day precision were both less than 8%. This developed method is successfully used in pharmacokinetic study of dasatinib in rats.

Słowa kluczowe

EN

LC-ESI-MS; rat plasma; gradient elution; dasatinib

• Czasopismo: Acta Chromatographica
• Rocznik: 2015
• Tom: Vol. 27, no. 1
• Strony: 81--91
• Opis fizyczny: Bibliogr. 13 poz., rys., tab.

Twórcy

autor

Wen, C.

• Wenzhou Medical College Laboratory Animal Centre Wenzhou 325035 China

autor

Zhang, Q.

• Shanghai Institute of Pharmaceutical Industry Shanghai 200437 China

autor

He, Y.

• Wenzhou Medical College Laboratory Animal Centre Wenzhou 325035 China

autor

Deng, M.

• Wenzhou Medical College Laboratory Animal Centre Wenzhou 325035 China

autor

Wang, X.

• Wenzhou Medical College Laboratory Animal Centre Wenzhou 325035 China

autor

Ma, J.

• Wenzhou Medical College Function Experiment Teaching Center Wenzhou 325035 China,

Bibliografia

• [1] N.P. Shah, C. Tran, F.Y. Lee, P. Chen, D. Norris, and C.L. Sawyers, Science, 305, 399–401 (2004)
• [2] T. O’Hare, D.K. Walters, E.P. Stoffregen, T. Jia, P.W. Manley, J. Mestan, S.W. Cowan-Jacob, F.Y. Lee, M.C. Heinrich, M.W. Deininger, and B.J. Druker, Cancer Res., 65, 4500–4505 (2005)
• [3] M.R. Burgess, B.J. Skaggs, N.P. Shah, F.Y. Lee, and C.L. Sawyers, Proc. Natl. Acad. Sci. U. S. A., 102, 3395–4000 (2005)
• [4] J.J. Berzas Nevado, G. Castaneda Penalvo, and F.J. Guzman Bernardo, J. Sep. Sci., 28, 543–548 (2005)
• [5] R.L. Oostendorp, J.H. Beijnen, J.H. Schellens, and O. Tellingen, Biomed. Chromatogr., 21, 747–754 (2007)
• [6] K. Titier, S. Picard, D. Ducint, E. Teilhet, N. Moore, P. Berthaud, F.X. Mahon, and M. Molimard, Ther. Drug Monit., 27, 634–640 (2005)
• [7] L. Wang, L.J. Christopher, D. Cui, W. Li, R. Iyer, W.G. Humphreys, and D. Zhang, Drug Metab. Dispos., 36, 1828–1839 (2008)
• [8] D.K. Hiwase, V. Saunders, D. Hewett, A. Frede, S. Zrim, P. Dang, L. Eadie, L.B. To, J. Melo, S. Kumar, T.P. Hughes, and D.L. White, Clin. Cancer Res., 14, 3881–3888 (2008)
• [9] E. Pirro, S. De Francia, F. De Martino, C. Fava, S. Ulisciani, G.R. Cambrin, S. Racca, G. Saglio, and F. Di Carlo, J. Chromatogr. Sci., 49, 753–757 (2011)
• [10] A.V. Kamath, J. Wang, F.Y. Lee, and P.H. Marathe, Cancer Chemother. Pharmacol., 61, 365–376 (2008)
• [11] S. De Francia, A. D’Avolio, F. De Martino, E. Pirro, L. Baietto, M. Siccardi, M. Simiele, S. Racca, G. Saglio, F. Di Carlo, and G. Di Perri, J Chromatogr., B: Anal. Technol. Biomed. Life Sci. 877, 1721–1726 (2009)
• [12] Y. Hsieh, G. Galviz, Q. Zhou, and C. Duncan, Rapid Commun. Mass Spectrom., 23, 1364–1370 (2009)
• [13] A. Haouala, B. Zanolari, B. Rochat, M. Montemurro, K. Zaman, M.A. Duchosal, H.B. Ris, S. Leyvraz, N. Widmer, and L.A. Decosterd, J. Chromatogr., B: Anal. Technol. Biomed. Life Sci. 877, 1982–1996 (2009)