Application of diffuse reflectance infrared Fourier transform spectroscopy for the detection of bacterial biomarkers in dry corrosion deposits

• Autorzy: Turek-Szytow, Jolanta , Hryniszyn, Alicja , Cwalina, Beata

Warianty tytułu

Zastosowanie spektroskopii z transformacją Fouriera w podczerwieni do wykrywania biomarkerów bakteryjnych w suchych osadach korozyjnych

• Języki publikacji: EN

Abstrakty

EN

Infrared spectroscopy (IR) may be used to indicate the presence of microorganisms in various deposits including these formed inside the drinking water distribution systems (DWDSs). The aim of this study was to examine the presence and intensity of absorption bands characteristic for dipicolinic acid (DPA) and other biomarkers of bacteria which probably formed biofilms in old, naturally dried deposits collected from corroded steel and cast iron pipes. The diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) was used to examine the presence and intensity of absorption bands that are characteristic of biomolecules deriving from bacterial cells. Our results showed a different chemical composition of deposits collected from various fragments of pipes and the presence of the microorganisms residues. We showed the possibility of using the DRIFT technique for rapid determination of biological contamination of dry corrosion deposits present in DWDSs. Analysis of the IR spectra second derivatives enabled for the precise determination of absorption maxima that were used to indicate the presence of amides, polypeptides, and fatty acids.

PL

Spektroskopia w podczerwieni (IR) może być wykorzystywana do wykazania obecności mikroorganizmów w różnych osadach, w tym tych powstałych w systemach dystrybucji wody pitnej (DWDS). Celem pracy było zbadanie obecności i intensywności pasm absorpcyjnych charakterystycznych dla kwasu dipikolinowego (DPA) i innych biomarkerów bakterii, które prawdopodobnie tworzyły biofilm w starych, naturalnie odwodnionych osadach pobranych ze skorodowanych rur stalowych i żeliwnych. Do zbadania obecności i intensywności pasm absorpcyjnych charakterystycznych dla biocząsteczek pochodzących z komórek bakteryjnych wykorzystano spektroskopię rozproszonego odbicia w zakresie podczerwieni z transformatą Fouriera (DRIFT). Badania wykazały zróżnicowany skład chemiczny osadów pobranych z różnych fragmentów rur oraz obecność pozostałości mikroorganizmów. Pokazano możliwość wykorzystania techniki DRIFT do szybkiego oznaczania biologicznego skażenia suchych osadów korozyjnych występujących w DWDS. Analiza drugich pochodnych widm IR pozwoliła na precyzyjne określenie maksimów absorpcji, które wykorzystano do wskazania obecności amidów, polipeptydów i kwasów tłuszczowych.

Słowa kluczowe

PL

osady suche; pozostałości biofilmu bakteryjnego; spektroskopia DRIFT

EN

dry deposits; bacterial biofilm residues; DRIFT spectroscopy

• Czasopismo: Ochrona przed Korozją
• Rocznik: 2022
• Tom: nr 8
• Strony: 251--257
• Opis fizyczny: Bibliogr. 30 poz., tab., wykr.

Twórcy

autor

Turek-Szytow, Jolanta

• Department of Environmental Biotechnology, Silesian University of Technology, Akademicka 2, Gliwice, Poland,

autor

Hryniszyn, Alicja

• Centre of Biotechnology at Silesian University of Technology, Krzywoustego 8, Gliwice, Poland

autor

Cwalina, Beata

• Institute of Non-Ferrous Matals, Gliwice, Poland

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).

Identyfikatory

DOI

10.15199/40.2022.8.2