Micro-sample analysis of capsaicin by liquid chromatography-tandem mass spectrometry assay and application to oral absorption study

• Autorzy: Choi, Y. , Shin, B. S. , Kim, M. G. , Shin, S. W. , Choo, H. W. , Kim, T. H. , Jang, H. C. , Yoo, S. D.
• Języki publikacji: EN

Abstrakty

EN

Capsaicin has been reported to exhibit an inhibitory effect on the P-glycoprotein (P-gp) function in vitro. To investigate its concentration-dependent effect in vivo, a sensitive assay that can characterize the absorption and disposition of capsaicin needs to be developed. This study reports the development of a sensitive LC-MS/MS assay for the determination of capsaicin in mouse plasma. The sample pretreatment involved a one-step extraction of 20 μL plasma with t-butyl methyl ether. Separations were achieved on a C18 column and the detection was performed on an LC-ESI-MS/MS by multiple reaction monitoring. The assay was linear over a wide concentration range from 0.325 to 650 ng mL^-1 (r > 0.999), with a LLOQ of 0.325 ng mL^-1. The developed method was applied to i.v. (dose 0.325 and 0.65 mg kg) and oral absorption (dose 40 mg kg) studies in mice. After i.v. injection, the t1/2,λz, Vz and CLs ranged from 0.13–0.16 h, 127.6–141.8 mL, and 547.3–775.4 mL/h, respectively. After oral administration, a secondary peak was observed and the terminal half-life was prolonged (1.51 h). Capsaicin was poorly absorbed, with the absolute oral bioavailability (F) ranging from 1.02% to 1.56%. The developed assay may be useful in studies where sample volumes are limited.

Słowa kluczowe

EN

capsaicin; LC-MS/MS; bioavailability; pharmacokinetics

• Czasopismo: Acta Chromatographica
• Rocznik: 2013
• Tom: Vol. 25, no. 2
• Strony: 363--376
• Opis fizyczny: Bibliogr. 17 poz., rys., tab.

Twórcy

autor

Choi, Y.

• Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea

autor

Shin, B. S.

• Catholic University of Daegu College of Pharmacy Gyeongsan-si, Gyeongbuk Korea

autor

Kim, M. G.

• Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea

autor

Shin, S. W.

autor

Choo, H. W.

• Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea

autor

Kim, T. H.

• Catholic University of Daegu College of Pharmacy Gyeongsan-si, Gyeongbuk Korea

autor

Jang, H. C.

• Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea

autor

Yoo, S. D.

• Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea,

Bibliografia

• [1] G.A. Cordell and O.E. Araujo, Ann. Pharmacother., 27, 330 (1993)
• [2] M.J. Caterina, M.A. Schumacher, M. Tominaga, T.A. Rosen, J.D. Levine, and D. Julius, Nature, 389, 816 (1997)
• [3] I. Tolan, D. Ragoobirsingh and E.Y.S.A. Morrison, Phytother. Res., 15, 391 (2001)
• [4] C.C. Chou, Y.C. Wu, Y.F. Wang, M.J. Chou, S.J. Kuo, and D.R. Chen, Oncol. Rep., 21, 665 (2009)
• [5] S.P. Huang, J.C. Chen, C.C. Wu, C.T. Chen, N.Y. Tang, Y.T. Ho, C. Lo, J.P. Lin, J.G. Chung, and J.G. Lin, Anticancer Res., 29, 165 (2009)
• [6] M.Y. Kim, L.J. Trudel, and G.N. Wogan, Anticancer Res., 29, 3733 (2009)
• [7] K.C. Brown, T.R. Witte, W.E. Hardman, H. Luo, Y.C. Chen, A.B. Carpenter, J.K. Lau, and P. Dasgupta, PLos ONE, 5, 1 (2010)
• [8] T. Nabekura, S. Kamiyama, and S. Kitagawa, Biochem. Biophys. Res Commun., 327, 866 (2005)
• [9] O.M.E.A. Salam, O.A. Heikal, and S. El-Shenawy, Pharmacology, 73, 121 (2005)
• [10] D. Suresh and K. Srivasan, Indian J. Med. Res., 131, 682 (2010)
• [11] J. Lu and M. Cwik, J. Chromatogr. B, 701, 135 (1997)
• [12] R. Wolf, C. Huschka, K. Raith, W. Wohlrab, and R.H.H. Neubert, J. Liquid Chromatogr. Rel. Technol., 22, 531 (1999)
• [13] C.A. Reilly, D.J. Crouch, G.S. Yost, and A.A. Fatah, J. Anal. Toxicol., 26, 313 (2002)
• [14] S. Babbar, J.F. Marier, M.S. Moukassi, M. Beliveau, G.F. Vanhove, S. Chanda, and K. Bley, Ther. Drug Monit., 31, 502 (2009)
• [15] Q. Zhang, J. Hu, L. Sheng, and Y. Li, J. Chromatogr. B Anal. Technol. Biomed. Life Sci., 878, 2292 (2010)
• [16] S. Chanda, M. Bashir, S. Babbar, A. Koganti, and K. Bley, Drug Metab. Dispos., 36, 670 (2008)
• [17] B.S. Shin, S.W. Hwang, J.B. Bulitta, J.B. Lee, S.D. Yang, J.S. Park, M.C. Kwon, D.J. Kim, H.S. Yoon, and S.D. Yoo, J. Toxicol. Environ. Health-Part A, 73, 1586 (2010)