Struktura chemiczna, swoistość antygenowa i znaczenie w chorobotwórczości lipopolisacharydu (LPS, endotoksyna) na przykładzie bakterii Proteus vulgaris

• Autorzy: Różalski, A. , Torzewska, A. , Bartodziejska, B. , Babicka, D. , Kwil, I. , Perepelov, A.V. , Kondakova, A.N. , Senchenkova, S.N. , Knirel, Y. , Vinogradov, Evgeny V.

Warianty tytułu

EN

Chemical structure, antigenic specificity and the role in the pathogenicity of lipopolysaccharide (LPS, endotoxin) on Proteus vulgaris bacteria's example

• Języki publikacji: PL

Abstrakty

EN

Gram-negative bacteria of the genus Proteus from the family Enterobacteriaceae are opportunistic pathogens, which cause mainly wounds and urinary tract infections (UTI), the latter leading to severe complications, such as acute or chronic pyelonephrithis and formation of bladder and kidney stones. Virulence factors and properties of Proteus sp. mediating infectious process are swarming phenomenon, adherence due to the fimbriae or glycocalyx, flagella, invasiveness, urease, amino acids deaminases, proteases, hemolysins, capsular polysaccharide (CPS), and lipopolysaccharide (LPS). LPS is an integral component of cell wall of bacteria. It also represents the endotoxin which, after being released from bacterial cells, causes a broad spectrum of pathological effects leading in severe cases to the septic shock. Lipopolysaccharide consists of three parts - O- specific chain (O-antigen), core and lipid A; all of them have been studied in Proteus LPS. It has been documented that Proteus is an antigenically heterogeneous genus, principally because of structural differences in its O-specific polysaccharide chain of LPS. The serological classification of P. mirabilis and P. vulgaris shares 60 serogroups : 22 described for P. vulgaris, 33 characteristic for P. mirabilis and 5 common for both P. mirabilis and P. vulgaris. Serological classification of Proteus penneri still remains to be completed. Proteus O-antigens are branched or linear polysaccharides, built up of oligosaccharide repeating units, varying from a trisaccharide to a hexasaccharide. Acidic O-specific polysaccharides represent the majority of Proteus O-antigens; it was found that 80% of Proteus O-antigens were acidic. Uronic acids and amino sugars usually determine the serological specificity of Proteus O-antigens. Amino sugars in Proteus O-antigens are usually N-acetylated. In many O-antigens, sugars constituents carry an O-acetyl groups. Hexuronic acids either have free carboxyl group or are amidated with the a-amino group of amino acids - lysine, serine, alanine or threonine. Chemical and serological studies have been undertaken with the aim to understand on the molecular level the immunospecificity of Proteus LPS and its potential role during infection of bacteria. The O-antigens and O-antisera against Proteus with defined epitope specificity can be used for serodiagnosis and epidemiological studies. It was found that O-specific polysaccharide Proteus bacteria is involved in creation of glycocalyx which allows bacteria to grow in microcolony or in biofilm. Biofilm protects bacteria against action of antimicrobial agents and leukocytes, and it is also a organic gel-like surrounding contributing to stone formation. LPS from the S form of bacteria, containing all three regions also contributes to their resistance against bactericidal action of serum. The present review is mainly focused on the structure, specificity and biological function of Proteus vulgaris LPS.

Słowa kluczowe

PL

chemia węglowodanów; lipopolisacharyd; endotoksyna; badania chemiczne Proteus; badania immunobiologiczne Proteus

EN

endotoxin; lipopolysaccharide; Proteus vulgaris bacteria

• Czasopismo: Wiadomości Chemiczne
• Rocznik: 2002
• Tom: [Z] 56, 7-8
• Strony: 585-604
• Opis fizyczny: bibliogr. 60 poz.

Twórcy

autor

Różalski, A.

• Zakład Immunobiologii Bakterii, Instytut Mikrobiologii i Immunologii, Uniwersytet Łódzki, ul. Banacha 12/16, 90-237 Łódź

autor

Torzewska, A.

• Zakład Immunobiologii Bakterii, Instytut Mikrobiologii i Immunologii, Uniwersytet Łódzki, ul. Banacha 12/16, 90-237 Łódź

autor

Bartodziejska, B.

• Zakład Immunobiologii Bakterii, Instytut Mikrobiologii i Immunologii, Uniwersytet Łódzki, ul. Banacha 12/16, 90-237 Łódź

autor

Babicka, D.

• Zakład Immunobiologii Bakterii, Instytut Mikrobiologii i Immunologii, Uniwersytet Łódzki, ul. Banacha 12/16, 90-237 Łódź

autor

Kwil, I.

• Zakład Immunobiologii Bakterii, Instytut Mikrobiologii i Immunologii, Uniwersytet Łódzki, ul. Banacha 12/16, 90-237 Łódź

autor

Perepelov, A.V.

• ND. Zelinski Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, Moscow 117913, Russia

autor

Kondakova, A.N.

• ND. Zelinski Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, Moscow 117913, Russia

autor

Senchenkova, S.N.

• ND. Zelinski Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, Moscow 117913, Russia

autor

Knirel, Y.

• ND. Zelinski Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, Moscow 117913, Russia

autor

Vinogradov, Evgeny V.

• Institute of Biological Sciences, National Research Council, 100 Sussex Drive, Ottawa, Ont. Canada K l A 0R6

• Zakład Immunobiologii Bakterii, Instytut Mikrobiologii i Immunologii, Uniwersytet Łódzki, ul. Banacha 12/16, 90-237 Łódź

Bibliografia

• [1] C. Mohr O’Hara, F.W. Brenner, J.M. Miller., Clin. Microbiol Rev., 2000, 13, 534.
• [2] R. Belas, ASM News, 1992, 58. 1.
• [3] R. Belas, [w:] Urinary Tract Infections, Molecular Pathogenesis and Clinical Management, H.L.T. Mobley, J.W. Warren (Eds.), ASM Press, Washington DC 1996, s. 245.
• [4] D. Gygi, M. Mahbur Rahman, H.-Ch. Lai, R. Carlson, J. Guard-Peter, C- Hughes, Mol. Microbiol., 1995,17, 1167.
• [5] J.L. Penner, [w:] The Prokaryotes, A. Balows, H.G. Triiper, W. Harder, K.H. Schleifer (Eds.), Springer Veriag. Berlin 1992, s. 2849.
• [6] J.W. Warren, [w:] Urinary Tract Infections, Molecular Pathogenesis and Clinical Management, H.L.T. Mobley, J.W. Warren (Eds.), ASM Press, Washington DC 1996, s. 2.
• [7] B.W. Senior, G.A. Anderson, K.D. Morley, M.A. Ken; J. Infect., 1999, 38, 99.
• [8] H.L.T. Mobley, [w:] Urinary Tract Infections, Molecular Pathogenesis and Clinical Management, H.L.T. Mobley, J.W. Warren (Eds.), ASM Press, Washington DC 1996, s. 245.
• [9] A. Różalski, Z. Sidorczyk, K. Kotelko, Microbiol. Mol. Biol. Rev., 1997, 61, 65.
• [10] A. Różalski, I. Kwil, D. Babicka, A. Torzewska, M. Wykrota, B. Bartodziejska, Mikrobiologia Medycyna, 1999,18, 3.
• [11] A. Różalski, Adv. Clin. Exp. Med., 2002,11, 3.
• [12] A. Różalski, Post. Mikrobiol., 1995, 34, 289.
• [13] A. Różalski, ibid., 1996, 35, 497.
• [14] P-E. Jansson, [w:] Endotoxin in Health and Disease, H. Brade, S.M. Opal, S.N. Vogel, D.C. Morrison (Eds.). Marcel Dekker Inc. NY. Basel 1999, s. 155.
• [15] O. Holst, ibid., s. 115.
• [16] U. Zahringer, B. Lindner. E.Th. Rietschel. ibid., s. 93.
• [17] F. Kaufmann, The Bacteriology o f Enterobacteriaceae. The Williams and Wilkins, Baltimore 1996, s. 333.
• [18] J.L. Penner, J.N. Hennessy, J. Clin. Microbiol., 1980. 12, 77.
• [19] K. Zych, M. Kowalczyk, Y.A. Knirel, Z. Sidorczyk, Adv. Exp. Med. Biol., 2000, 485, 339.
• [20] P. Larsson, Methods Microbiol., 1984, 14, 187.
• [21] Y.A. Knirel., E.V. Vinogradov., A.S. Shashkov, Z. Sidorczyk, A. Różalski, J. Radziejewska-Lebrecht, W. Kaca, J. Carbohydr. Chem., 1993,12, 379.
• [22] Z. Sidorczy k, Post. Hig. Med. Dośw., 1996, 50. 401.
• [23] Y.A. Knirel, W. Kaca, A. Różalski, Z. Sidorczyk, Pol. J. Chem.. 1999. 73, 859.
• [24] Y.A. Knirel, A. Różalski i wsp., dane nieopublikowane.
• [25] A. Ziółkowski, A.S. Shashkov, A. Świerzko, S.N. Senchenkova, F.V. Toukach, M. Cedzyński, K.-I. Amano, W. Kaca, Y.A. Knirel. FEBS Lett., 1997, 411, 221.
• [26] A.V. Perepelov, S.N. Senchenkova, A.S. Shashkov, D. Babicka, H. Moll, A. Różalski, U. Zahringer, Y.A. Knirel, Carbohydr. Res., 2001, 331, 195.
• [27] A.N. Kondakova. A.V. Perepelov. B. Bartodziejska, A.S. Sahashkov, S.N. Senchenkova, M. Wykrota, Y.A. Knirel, A. Różalski, ibid., 2001. 333. 241.
• [28] A.V. Perepelov, A. Torzewska, A.S. Shashkov, S.N. Senchenkova, A. Różalski, Y.A. Knirel. Eur. J. Biochem., 2000, 267, 788.
• [29] D. Babicka, „Badania swoistości serologicznej antygenów O Proteus vulgaris O l, 0 2 ,0 4 i 08 oraz wybranych właściwości biologicznych tego gatunku” (praca doktorska), Zakład Immunobiologii Bakterii, Uniwersytetu Łódzkiego, Łódź 2001.
• [30] M. Cedzyński. A. Ziółkowski. A. Świerzko, Y.A. Knirel, K.-L. Amano, A. Różalski, W. Kaca, [w:] J. Kazar, R. Toman. Proc. Vth Intern. Symp. Rickettsiae and Rickettsial Diseases. Veda, Bratislava 1996, s. 261.
• [31] V. Perepelov, D. Babicka, A.S. Shashkov, N.P. Arbatsky, S.N. Senchenkova, A. Różalski, Y.A. Knirel, Carbohydr. Res., 1999, 318, 186.
• [32] A. Torzewska, „Polisacharydy O-swoiste Proteus vulgaris - swoistość serologiczna i znaczenie w powstawaniu kamieni moczowych” (praca doktorska). Zakład Immunobiologii Bakterii Uniwersytetu Łódzkiego, Łódź 2001.
• [33] Y.A. Knirel, W. Kaca. N.A. Paramonov. M. Cedzyński, E.V. Vinogradov, A. Ziółkowski, A.S. Shashkov, A. Różalski, Eur. J. Biochem., 1997, 247, 951.
• [34] E.V. Vinogradov, Z. Sidorczyk, A. Świerzko, A. Różalski, E.D. Daeva, A.S. Shashkov, Y.A. Knirel, N.K. Kochetkov, ibid., 1991, 197, 93.
• [35] M. Cedzyński, A.S. Świerzko, A. Ziółkowski, A. Różalski, N.A. Paramonov, E.V. Vinogradov, Y.A. Knirel, W. Kaca, Microbiol. Immunol., 1998, 42, 7.
• [36] B. Bartodziejska, A.S. Shasahkov, D. Babicka, A.A. Grachev, A. Torzewska, N.A. Paramonov. A.Y. Chemyak, A. Różalski. Y.A. Knirel, Eur. J. Biochem., 1998, 256, 488.
• [37] F.V. Toukach, B. Bartodziejska, S.N. Senchenkova, M. Wykrota, A.S. Shashkov, A. Różalski, YA. Knirel, Carbohydr. Res., 1999, 318, 146.
• [38] Y.A. Knirel, K. Zych, E.V. Vinogradov, A.S. Shashkov, Z. Sidorczyk, Eur. J. Biochem., 2000, 267, 815.
• [39] A.S. Shashkov, A.N. Kondakova, K. Zych, S.N. Senchenkova. F.V. Toukach. Z. Sidorczyk, Y.A. Knirel, Eur. J. Biochem., 2000, 267, 601.
• [40] B. Bartodziejska, F.V. Toukach, E.V. Vinogradov, S.N. Senchenkova, A.S. Shashkov, A. Ziółkowski, J. Czaja, M.B. Perry, Y.A. Knirel, A. Różalski, ibid., 2000, 267, 6888.
• [41] A.V. Perepelov, S.N. Senchenkova, A. Torzewska, B. Bartodziejska, A.S. Shashkov, A. Różalski, Y.A. Knirel, Carbohydr. Res., 2000, 328, 229.
• [42] A. Torzewska, A.N. Kondakova, A.V. Perepelov, S.N. Senchenkova, A.S. Shashkov, A. Różalski, Y.A. Knirel, FEMS Immunol. Med. Microbiol., 2001,31, 227.
• [43] L. Clapham, RJ.C. McLean, J.C. Nickel, J. Downey, J.W. Costerton, J. Crystal Growth., 1990, 104 475.
• [44] A J. Dumanski, H. Hedelin, A. Edin-Liljegren, D. Beauchemin, RJ.C. McLean, Infect. Immun., 1994. 62 2998.
• [45] W. Kaca, E. Ujazda, Post Mikrobiol., 1998, 37, 421.
• [46] W. Kaca, K.-I. Amano. A.Y. Chernyak, Y.A. Knirel, Microbios., 2000, 103, 151.
• [47] B. Bartodziejska, A. Torzewska, D. Babicka, M. Wykrota, A. Różalski, A.V. Perepelov, F.V. Toukach, AA. Grachev, S.N. Senchenkova, A.S. Shashkov, Y.A. Knirel, Adv. Exp. Med. Biol., 2000, 485, 243.
• [48] E.V. Vinogradov, J. Radziejewska-Lebrecht, Carbohydr. Res., 2000, 329, 351.
• [49] Z. Sidorczyk, U. Zähringer, E.Th. Rietschel, Eur. J. Biochem., 1983,137, 15.
• [50] E.V. Vinogradov, E.V., E. Thomas-Oates, H. Brade, O. Holst, J. Endotoxin Res., 1994,1,199.
• [51] E. Vinogradov, M. Cedzyński, A. Różalski, A. Ziółkowski, A. Świerzko, Carbohydr. Res., 2000, 328, 533.
• [52] A. Różalski, Post. Mikrobiol., 1991, 30, 301.
• [53] C. Ługowski, T. Niedziela, W. Jachymek. Mikrobiologia Medycyna, 1996, 4, 28.
• [54] M. Mahbubur Rhaman, J. Guard-Petter, K. Asokan, C. Hughes, J. Biol. Chem., 1999, 274, 22293.
• [55] L.M. Beynon, D J. Dumanski, RJ.C. McLean, L.L MacLean, J.C. Richards, M.B. Peny, J. Bacteriol., 1992,174, 2172.
• [56] M.M. Rahman, J. Guard-Petter, K. Asokan, R W. Carlson, Carbohydr. Res., 1997, 301, 213.
• [57] E.Th. Rietschel, H. Brade, Sci. Am., 1992, 239, 26.
• [58] E.Th. Rietschel, T. Kirikae, U. Schade, A J. Ulmer, O. Holst, H. Brade, G. Schmidt, U. Mamat, H.D. Grimmecke, S. Kusumoto, U. Zahringer, Immunobiology, 1993,187, 169.
• [59] M.B. Peny, L.L. MacLean, Carbohydr. Res., 1994, 253, 257.
• [60] A.V. Perepelov, A.S. Shashkov, D. Babicka, S.N. Senchenkova, B. Bartodziejska, A. Różalski, Y.A. Knirel, Biochemistry (Moscow), 2000, 65, 1247.