Conducting Polymer Films as Model Biological Membranes. Electrochemical and Ion-Exchange Properties of PPy and PEDOT Films Doped with Heparin

• Autorzy: Paczosa, B. , Błaż, T. , Migdalski, J. , Lewenstam, A.
• Języki publikacji: EN

Abstrakty

EN

The interaction of calcium, magnesium potassium and sodium cations with heparin (Hep), a highly negatively charged glycosaminoglycan, was studied by using conducting polymer (CP) films. The CP-Hep films were obtained by electrochemical deposition of poly(pyrrole) (PPy) or poly(3,4-ethylenedioxythiophene) (PEDOT). In order to induce magnesium or calcium sensitivity, all films were conditioned in alkaline solution containing magnesium or calcium ions, respectively. After conditioning, the potentiometric response towards calcium or magnesium ions with a close-to-Nernstian slope was observed. The calcium or magnesium sensitive CP-heparin films do not respond to Na or K ions, but the response towards Mg or Ca ions was almost identical. Adistinct difference in dynamic open-circuit response to calcium or magnesium ions was observed and its relevance to biological membrane processes is stressed. CP-heparin films can be used for testing an ion exchange as well as transport processes that occur in biological membranes.

Słowa kluczowe

EN

poly(pyrrole)(PPy); poly(3,4-ethylenedioxythiopene (PEDOT); heparin; membrane potential; action potential; biological cell membrane

• Czasopismo: Polish Journal of Chemistry
• Rocznik: 2004
• Tom: Vol. 78 / nr 9
• Strony: 1543-1552
• Opis fizyczny: Bibliogr. 17 poz., rys.

Twórcy

autor

Paczosa, B.

• Faculty of Material Science and Ceramics, AGH-University of Science and Technology, al. Mickiewicza 30, PL-30059 Cracow, Poland

autor

Błaż, T.

• Faculty of Material Science and Ceramics, AGH-University of Science and Technology, al. Mickiewicza 30, PL-30059 Cracow, Poland

autor

Migdalski, J.

• Faculty of Material Science and Ceramics, AGH-University of Science and Technology, al. Mickiewicza 30, PL-30059 Cracow, Poland

autor

Lewenstam, A.

• Faculty of Material Science and Ceramics, AGH-University of Science and Technology, al. Mickiewicza 30, PL-30059 Cracow, Poland
• Center for Process Analytical Chemistry and Sensor Technology (ProSens), c/o Process Chemistry Center of Excellence, Åbo Akademi University, FIN-20500 Turku-Åbo, Finland

Bibliografia

• 1. Bobacka J., Ivaska A. and Lewenstam A., Electroanalysis, 15, No 5-6, 1 (2003).
• 2. Migdalski J., Blaz T. and Lewenstam A., Anal. Chim. Acta, 322, 141 (1996).
• 3. Migdalski J., Blaz T. and Lewenstam A., Chem. Anal. (Warsaw), 47, 371 (2002).
• 4. Migdalski J., Chem. Anal. (Warsaw), 47, 595 (2002).
• 5. Migdalski J., Blaż T., Paczosa B. and Lewenstam A., Mikrochim. Acta, 143,177 (2003).
• 6. Hepel J., Bruckenstein S. and Hepel M., Microchem. J., 55,179 (1997).
• 7. Zhou D., Too C.O. and Wallace G.G., React. Fund. Polym., 39,19 (1999).
• 8. Boyle A., Genes E. and Fouletier M., J. Eledroanal. Chem., 279, 179 (1990).
• 9. Pyo M., Maeder G., Kennedy R.T. and Reynolds J.R., J. Eledroanal. Chem, 368, 329 (1994).
• 10. Desai U.R., Med. Res. Rev., 24, 151 (2004).
• 11. Meyerhof M.E., Anal. Chem., 64, 694 (1992).
• 12. Kvamstrom C., Neugebauer H., Ivaska A. and Sariciftci N.S., J. Mol. Struct., 521, 271 (2000).
• 13. Randriamahazaka H., Noel V. and Chevrot C., J. Eledroanal. Chem., 521, 107 (2002).
• 14. Maj-Zurawska M. and Lewenstam A., Anal. Chim. Ada, 236, 331 (1990).
• 15. Lewenstam A., Maj-ZurawskaM., Blomqvist N. and Ost J., Clin. Chem. Enzym. Comm., 5,95 (1993).
• 16. Lewenstam A., Hulanicki A. and Sokalski T., Anal. Chem., 59, 1539 (1987).
• 17. Sokalski T., Lingenfelter P. and Lewenstam A., J. Phys. Chem. B, 117, 2443 (2003).