A biochemical model for characterising the surface-active phospolipid bilayer of articular cartilage relative to acid-base equilibrium

• Autorzy: Pawlak, Z. , Kotynska, J. , Figaszewski, Z. , Gadomski, A. , Gudaniec, A. , Oloyede, A.
• Języki publikacji: PL

Abstrakty

EN

Purpose: This paper, addresses the question of how changes in acid - base equilibrium influence change in the charge density of the phospholipid bilayer on articular cartilage surfaces during lubrication. Design/methodology/approach: Liposomes have been used to mimic biological phospholipid membranes on articular cartilage surface where proteins are bounded, ions are transported, energy is transducted, and cellular processes take place. The charge density of the membrane was determined as a function of pH and electrolyte concentration from the microelectrophoretic method. Liposome membrane was prepared as an aqueous solution of NaCl under various pH conditions. Microelectrophoresis was used to examine the local acid-base equilibrium of the electrolytes with the membrane surface, which can be considered to model the phospholipids interface in articular cartilage. Findings: The adsorbed ions (H+, OH-, Na+, Cl-) which are present in the electrically charged solutions of liposome membrane comprising phosphatidycholine (PC), were found to exhibit pH-responsive quasi-periodic behavior. Research limitations/implications: We have established that the acid-base dissociation behavior in phospholipid bilayers of articular cartilage is a key to understanding biolubrication processes. For example,previous investigators found that the formation of the multilayer of polyisopeptide/hyaluronic acid depends on surface properties such as film thickness, surface friction, surface wetability; wetness and swelling behavior. Future work should consider the adsorption of polyelectrolyte ions, e.g., the glycoprotein lubricin and hyaluronan, on the liposome membrane surface in the presence of H+ and OH- ions. Originality/value: A novel model of the joints’ phospholipid bilayers has been created using liposome membrane This model can be applied in the investigation of polyelectrolyte ions such as lubricin, in articular cartilage. We have demonstrated that the acid-base processes on charged surfaces is a key mechanism in facilitating lubrication in human joints.

Słowa kluczowe

PL

biomateriały; chrząstka stawowa; własności elektryczne; fosfolipidy membranowe; pH

EN

biomaterials; electrical properties; lubrication; articular cartilage; surface charge density; phospholipids membrane; pH

• Czasopismo: Archives of Materials Science and Engineering
• Rocznik: 2008
• Tom: Vol. 29, nr 1
• Strony: 24-29
• Opis fizyczny: Bibliogr. 39 poz.

Twórcy

autor

Pawlak, Z.

autor

Kotynska, J.

autor

Figaszewski, Z.

autor

Gadomski, A.

autor

Gudaniec, A.

autor

Oloyede, A.

• University of Technology and Life Sciences, ul. Seminaryjna 3, 85-326 Bydgoszcz, Poland,

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