Warianty tytułu
Bioaugmentation of phenol-contaminated soil with pseudomonas vesicularis (pBR322)
Języki publikacji
Abstrakty
Celem pracy było zbadanie rozkładu fenolu w dwóch glebach (L1 i L2), różniących się zawartością materii organicznej, poddanych bioaugmentacji z użyciem szczepu Pseudomonas vesicularis (pBR322). Przeprowadzone obserwacje biodegradacyjne wykazały, że w poddanej bioaugmentacji glebie leśnej (L1) zawierającej 34,68% materii organicznej, rozkład fenolu w stężeniu 1,7 mg g-1 gleby zachodził w ciągu 8 dni, natomiast w piaszczystej glebie leśnej (L2) o niskiej zawartości materii organicznej (2,03%) w ciągu 12 dni. Jednocześnie stwierdzono, że P. vesicularis (pBR322) oraz bakterie heterotroficzne lepiej przeżywały w skażonej fenolem glebie L1 niż w L2. W 8 dniu inkubacji w glebie L1 oznaczono około 80% wprowadzonej liczby komórek zarówno P. vesicularis (pBR322) jak i początkowej liczby bakterii hetero-troficznych, natomiast w glebie L2 w 12 dniu było to odpowiednio 66% i 75%. Potwierdzono także stabilność wprowadzonego do inokulowanych komórek P. vesicularis plazmidu pBR322 w trakcie badań biodegradacyjnych.
The aim of this study was to investigate phenol degradation in two soils (LI and L2) varying in organie matter content and bioaugmented using Pseudomonas vesicularis (pBR322) strain. The biodegradation experiments showed that phenol was degraded in bioaugmented forest soil (LI) containing the high amount of organic matter (34.68%) within 8 days, whereas in sandy forest soil (L2) with low organic matter content (2.03%) - within 12 days. Moreover, it was found that in phenol-contaminated LI soil, introduced P. vesicularis (pBR322) cells and heterotrophic bacteria survived better than in L2 soil. On day 8 about 80% of inoculated cells as well as the initial number of heterotrophic bacteria was determined in phenol contaminated LI soil, whereas in L2 soil on day 12 about 66% and 75%, respectively. It was also confirmed that plasmid pBR322 in P. vesicularis cells was stable during whole experimental period.
Czasopismo
Rocznik
Tom
Strony
87-93
Opis fizyczny
Bibliogr. 29 poz., tab., rys.
Twórcy
autor
autor
- Uniwersytet Śląski, Wydział Biologii i Ochrony Środowiska, Katedra Biochemii, 40-032 Katowice, ul. Jagiellońska 28
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.baztech-article-BPG8-0028-0012