Methods of examination of genotoxicity of atmospheric pollutants

• Autorzy: Jadczyk, P.

Warianty tytułu

PL

Metody badania genotoksyczności zanieczyszczeń atmosfery

• Języki publikacji: EN

Abstrakty

EN

Current physicochemical monitoring of atmospheric pollutants fully reflects their biological activity. This should be supplemented with evaluation of mutagenicity and carcinogenicity of those pollutants by means of biotests. Test selection should take into consideration the sensitivity of test organisms to genotoxic activity of atmospheric pollutants, conformity of the results with those of other biotests, common use of environmental mutagenesis and carcinogenesis in the tests with special consideration of atmospheric pollutants, possibility of examining the activity of atmospheric pollutants independent of atmospheric conditions, short duration of the test cycle, relatively low cost, reducing the suffering of laboratory animals to minimum. Those criteria are best fulfilled by the following tests on microorganisms and tissue cultures: the Ames test with Salmonella typhimurium, sister chromatide exchange induction and canceration in cells of human respi-ratory systems and identification of DNA adducts in human organisms.

PL

Stosowany obecnie monitoring fizykochemicznych zanieczyszczeń atmosfery nie odzwierciedla w pełni ich aktywności biologicznej. Powinien on być uzupełniony oceną mutagenności i kancerogenno-ści tych zanieczyszczeń za pomocą biotestów. Wybierając biotesty, należy wziąć pod uwagę: wrażliwość testowanych organizmów na genotoksyczne działanie atmosfery, zgodność otrzymanych wyników z wynikami innych biotestów, powszechność stosowania testów w badaniu mutagenezy i kancerogenezy środowiskowej ze szczególnym uwzględnieniem zanieczyszczeń atmosfery, możliwość badania aktywności zanieczyszczeń atmosfery niezależnie od warunków atmosferycznych, krótkotrwałość cyklu badawczego, relatywnie niski koszt oraz ograniczenie do niezbędnego minimum cierpienia zwierząt laboratoryjnych. Kryteria te w największym stopniu spełniają testy na mikroorganizmach i kulturach tkankowych: test Amesa na Salmonella typhimurium, indukowanie wymiany chromatyd siostrzanych i transformacji nowotworowej w komórkach narządów oddechowych człowieka oraz identyfikacja adduktów DNA u ludzi.

• Czasopismo: Environment Protection Engineering
• Rocznik: 2003
• Tom: Vol. 29, nr 3-4
• Strony: 97-112
• Opis fizyczny: Bibliogr. 94 poz.

Twórcy

autor

Jadczyk, P.

• Institute of Environment Protection Engineering, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland.

Bibliografia

• [1] ADAMIAK W., JADCZYK P., KUCHARCZYK J., Application of Salmonella Strains with Altered Nitroreductase and O-acetyltransferase Activities to the Evaluation of the Mutagenicity of Airborne Particles, Acta Microbiol. Polon., 1999, 48, 131-140.
• [2] ALINK G.M., SMIT H.A., VAN HOUDT J.J., KOLKMAN J.R., BOLEIJ J.S.M., Mutagenic activity of airborne particulates at non-industrial locations, Mutation Res., 1983, 116, 21-34.
• [3] BARALE R., МIGLIORE L., CELLINI B., FRANCOM! L., GIOGELLI F., BARRAI N., LOPRIENO N., Genetic Toxicology of Airborne Particulate Matter Using Cytogenetic Assays and Microbial Mutagenicity Assay, Genetic Toxicol of Complex Mixtures (Plenum), 1990, 57-72.
• [4] BAYONA J.M., CASELLAS M., FERNANDEZ P.P., SOLANAS A.M., ALBAIGES J., Sources and seasonal variability of mutagenic agents in the Barcelona city aerosol, Chemosphere, 1994, 29, 441-450.
• [5] BINKOVA B., VESELY D., VESELA D., JELINEK R., SRAM R.J., Genotoxicity and embryotoxicity of urban air particulate matter collected during winter and summer period in two different districts of the Czech Republic, Mutation Res., 1999, 440, 45-58.
• [6] BLAMER W., TSAI W.-Y., CHORĄŻY M., MOTYKIEWICZ G., MICHALSKA J., SANTELLA R.M., YOUNG T.-L., Molecular and Genetic Damage in Humans from Environmental Pollution in Poland, Nature, 1992, 360, 256-258.
• [7] BOLOGNESI G., MERLO F., RABBONI R., VALERIO F., ABBONDANDOW A., Cytogenetic Biomonitoring in Traffic Police Workers - Micronucleus Test in Peripheral Blood Lymphocytes, Environ. Molecular Mutagenesis, 1997, 30, 396-402.
• [8] CERNA M., PASTORKOVA A., MYERS S.R., ROSSNER P., BINKOVA В., The use of urinary mutagenicity assay in the monitoring of environmental exposure to genotoxins, Mutation Res., 1997, 391, 99-110.
• [9] CHOUROULINKOV I., Carcinogenecity of Complex Mixtures in Air Using in Vitro and in Vivo Assays, Genetic Toxicol. of Complex Mixtures (Plenum), 1991, 113-126.
• [10] COULOMB H.M., COURTOIS Y.A., CALLAIS F.B., FESTY B., CHOUROLINKOV I., Biological activity of air particulate extracts (APE) in short-term tests, [in:] Waters M.D., Sandhu S.S., Lewtas J., Claxton L., Chernoff N., Nesnow S. (Eds.), Short-Term Bioassays in the Analysis of Complex Environmental Mixtures, III Plenum, New York, 1983, 485-497.
• [11] Courtois Y.A., Min S., LACHENAL C., JACQUOT-DESCHAMPS J.M., CALLAIS F., FESTY B., Genotoxicity of Organic Extracts from Atmospheric Particles, NY Acad. of Sci. Annals: Living in Chem. World Conf., Bologna, Italy, 1988, 534, 724-741.
• [12] CREBELLI R., FUSELLI S., BALDASSARRI L.T., ZIEMACKI G., CARERE A., BANIGNI R., Genotoxicity of urban air particulate matter: correlations between mutagenicity data, airborne micropollutants, and meteorological parameters, Int. J. Environment. Health Res., 1995, 5, 19-34.
• [13] DEHNEN W., PITZ N., TOMINGAS R., The mutagenicity of airborne particulate pollutants, Cancer Lett., 1997, 4, 5-12.
• [14] DEPOLLOK F.S., ANEJA V.P., HUGHES T.J., CLAXTON L.D., Chemical and mutagenic analysis of volatile organic compounds in Raleigh air samples at 3 different elevations before, during and after Hurricane Cordon , Chemosphere, 1997, 35, 879-893.
• [15] ENYA T., SUZUKI H., WATANABE M., HIRAYAMA T., HISAMATSU Y., 3-Nitrobenzanthrone, a Power­ ful Bacterial Mutagen and Suspected Human Carcinogen Found in Diesel Exhaust and Airborne­ Particulates , Environ. Sci. Technol., 1997, 31, 2772-2776.
• [16] FREEMAN A .E., PRICE P.J., BRYAN R.J., GORDON R.J., GILDEN R.V., KELLOFF G.J., HUEBNER R.J., Transformation of rat and hamster embryo cells by extracts of city smog , Proc. Natl. Acad. Sci. USA, 1971 , 68, 445-449.
• [17] FUKINO H., MIMURA S.,INOURE K., Y AMANE Y., Mutagenicity of airborne particles, Mutation Res., 1982. 102,237-247.
• [18] FUSELLI S., BEGIGNI R., CONTI L., CARERE A., CREBELLI R., Volatile organic compounds (VOCs) and air mutagenicity: results of one year monitoring at an urban site, Int. J. Health Res., 1995, 5, 123-1 32.
• [19] GORDON R.J., BRYAN R.J., RHIM J.S., DEMOISE C., WOLFORD R.G., FREEMAN A.E., HUEBNER R.J., Transformation of rat and mouse embryo cells by a new class of carcinogenic compounds isolated from particles in city air, Int. 1 . Cancer., 1973, 12, 223-232.
• [20] GRANT W.F., Higher plant assay for the detection of genotoxicity in air polluted environments, Ecosystem Health, I998, 4, 210-229.
• [21] HADNAGY W., SEEMAYER N.H., TOMINGAS R., Cytogenetic effects of airborne particulate matter in human lymphocytes in vitro, Mutation Res., 19 86, 175,97-101.
• [22] HADNAGY W., SEEMAYER N.H., TOMINGAS R., I VANFY K., Comparative study of sister-chromatid exchanges and chromosomal aberrations induced by airborne particulate from an urban and a highly industrialized location in human lymphocyte cultures , Mutation Res., 1 989, 225, 27-32.
• [23] HANNIGAN M.P., CASS G.R., PENMAN B.W., CRESPI Ch.L., LAFLEUR A.L., BUSBY W.F., THILLY W.G., Human Cell Mutagens in Los Angeles Air, Environ. Sci. Technol., 1997, 31, 438-447.
• [24] HEMMINKI K., KUMAR R., BYKOV V.J., LOUHELAINEN J., VODICKA P., Future Research Directions in the Use of Biomarkers, Environ. Health Persp., 1996, I 04, 459-463.
• [25] VAN'T HOF J., SCHREIER L.A., Tradescantia assay system for gaseous mutagens, are port of U.S. EPA Gene-Tax Program, Mutation Res., 1982, 99, 303-315.
• [26] HORNBERG C., MACIULEVICIUTE L., SEEMAYER N.H., Comparative Analysis of Cyto- and Genotoxic Effects of Airborne ?articulates on Human and Rodent Respiratory Cells in Vitro, Toxic. in Vitro, 1997, 11,7 11-715.
• [27] HORNBERG C., SEEMAYER N.H., Tracheal Epithelial Cells in Vitro as a Model to Stud y Genotoxicity of Airborne ?articulates, Toxic. in Vitro, 1995, 9, 397-402.
• [28] HUGHES T.J., LEWTAS J., CLAXTON L.D., Development of a Standard Reference Material for Diesel Mutagenicity in the Salmonella Plate Incorporation Assay, Mutation Res., 1997, 39 1, 243-258.
• [29] INDULSKI J. (Ed.), Guide to short -term test s for detecting mutagenic and carcinogenic chemicals (in Polish), Environmental Health Criteria, Vol. 5 I , Institute of Occupational Medicine, Łódź, 1993.
• [30] JADCZYK P., The mutagenicit y of organic pollutant s adsorbed on airborne particles in the center of Wroclaw (in Polish), Roczn. PZH, 2000, 51, 299-305.
• [31] JADCZYK P., Correlations between mutagenicity of airborne particles and concentrations of air pollutants (in Polish), Roczn. PZH, 200 l, 5, 25-33.
• [32] JADCZYK P., Effect of traffic in Wroclaw town centre on mutagenicty of airborne particulate matter, Acta Pol. Toxicol., 2001,9,57-67.
• [33] JADCZYK P., Differention of mutagenicity of pollutants adsorbed on airborne particles in urban environment of Wroclaw (in Polish), [in:] Górecki H., Kochman M., Ożyhar A., Zwodziak J. (Eds.), I Sympozjum Centrum Biomonitoringu, Biotechnologi.i i Ochrony Ekosystemów Dolnego Ślaska, Wroclaw, 4-5 kwietnia 2001 , 181-1 85.
• [34] JADCZYK P., KUCHARCZYK J., Mutagenicity of micropollutants adsorbed on the airborne particles in the centre of Wrocław (in Polish), [in:] Janosz-Rajczyk М. (Ed.), Micropollutants in the human environment, Częstochowa-Ustгoń, 8-10 September 1999, Częstochowa. Wyd. P. Częst., 107-112.
• [35] JADCZYK P., KUCHARCZYK J., Seasonal variability of the mutagenicity of airborne particles for Salmonella typhimurium ТА 98 and Saccharomyces cerevisiae XV 185-14C, ЕPЕ, 2000, 26, 89-102.
• [36] KARРIŃSKA -SMULIKOWSKA J., PIEKARSKA K., MOSKAL J., CZARNY A., Evaluation of air pollution by bioindication tests, Acta Pol. Toxicol., 2000, 8, 147-159.
• [37] KRISHNA G., NATH J., ONG T., Correlative genotoxicity studies of airborne particles in Salmonella typhimurium and cultured lymphocytes, Environ. Mutagen., 1984, 6, 584-592.
• [38] KROGULSKI A., BORKOWSKA M., The method of testing the mutagenie activity of coal tar in atmospheric dust (in Polish), Roczn. PZH, 1996, 47, 175-180.
• [39] KROGULSKI A., BORKOWSKA M., SТRUSIŃSKA A., Mutagenie activity of atmospheric dust in Warsaw (in Polish), Roczn. PZH, 1997, 48, 31-35.
• [40] KROGULSKI A., BORKOWSKA M., SТRUSIŃSKA A., Determination of the mutagenie activity of cyclohexane extract of atmospheric air dust and polycyclic aromatic hydrocarbons on various strains of Drosophila melanogaster, Roczn. PZH, 1998, 49, 279-283.
• [41] MA T.Н., ANERSON V.A., AHMED I., Environmental clastogens detected by meiotic pollen mother cells of Tradescantia, [in:] Tice R.R., Costa D.L., Schaich KM, (Eds.), Cenoloxic Effects of Airborne Agents, Plenum Press, NY, 1982, 141-157.
• [42] MA T.H., Tradescantia-micronucleus (Trad-MNC) test for environmental clastogens, [in:] Kolber A.R., Wong T.K., Grant L.D., DeWoskin R.S., Hughes T.J. (Eds.), In vitro Toxicity Testing of Environmental Agents. Current and Future Possibilities, Plenum Press, NY, 1983, 191-214.
• [43] MA T.H., Application of quick and sample plant bioassays to assess the genotoxicity of environmental pollutants: detection of potential health hazards of air, water, soil contaminations, [in:] Proc. EUROTOX Congress, Toxicology in Transition, Degen G.H., Seiler J.P., Bentley P. (Eds.), Basel, Schwitzerland, August 21-24, 1994, Springer, 420-433.
• [44] MA T.H., HARRIS M.M., ANDERSON V.A., AHMED I., MOHAMMAD K., BARE J.L., LIN G., Tradescantiamicronucleus (Trad-MCN) tests on 140 health related agents, Mutation Res., 1984, 138, 157-167.
• [45] MA T.H., Xu C., LIAO S., MCCONNELL H., JEONG B.S., WON C.D., in situ monitoring with the Tradescantia bioassays on the genotoxicity of gaseous emissions from a closed landfill site and incinerator, Mutation Res., 1996, 359, 39-52.
• [46] MADSEN E.S., NIEISEN P.A., PEDERsEN J.Ch., The distribution and origin of mutagens in airborne particulates, detected by Salmonella/microsome assay in relation to levels of lead, vanadium and РАН, Science Total Environ., 1982, 24, 13-25.
• [47] MARON D.M., Ames B.N., Revised metods for the Salmonella mutagenicity test, Mutation Res., 1983,113,173-215.
• [48] MATSUMOTO H., INOUE K., Mutagenicity of a Polar Portion in the Neutral Fraction Separated from Organic Extracts of Airborne Particulates, Arch. Environ. Contam. Toxicol., 1987, 16, 409-416.
• [49] MATSUMOTO Y., SAKAI S., KATO T., NAKAJIMA T., SATOH H., Long-Term Trends of Particulate Mutagenie Activity in the Atmosphere of Sapporo. Determination of Mutagenie Activity by the Conventional Tester Strains TА98 and TA100 during an 18-Year Period (1974-1992), Environ. Sci. Technol., 1998, 32, 2665-2671.
• [50] MENCK H.R., CASAGRANDE J.T., HENDERSON B.E., Industrial Air Pollution: Possible Effect on Lung Cancer, Science, 1974, 183, 210-212.
• [51] МICIЕТA K., MLJRIN G., Microspore analysis for genotoxicity of polluted environment, Environ. Exp. Botany, 1996, 36, 21-27.
• [52] MIСIEТA K., MURIN G., Three species of genus Pinus suitable as bioindicators of polluted environment, Water, Air, Soil Poll., 1998, 104, 413-422.
• [53] MOLLER M., ALFHEIM I., Mutagenicity of air samples from various combustion sources, Mutation Res., 1983, 116, 35-46.
• [54] MORRIS W.A., VERSTEE° J.K., BRYANT D.W., LEGZIDINS A.E., MCCARRY B.E., MARVIN C.H., Preliminary Comparisons between Mutagenicity and Magnetic Susceptibility of Respirable Airborne Particulate, Atmos. Environ., 1995, 29, 3441-3450.
• [55] MOTYKIEWICZ G., CIMANDER B., SZELIGA J., ТKOCZ A., СHORAZY M., Mutagenic activity of complex air pollutants in Silesia, Complex Mixtures and Cancer Risk, 1990, 261-268.
• [56] MOTYKIEWICZ G., HADNAGY W., SEEMAYER N.H., SZELIGA J., ТKOCZ A., CHORAŻY М., Influence of airborne suspended matter on mitotic cell division, Mutation Res., 1991, 260, 195-202.
• [57] MOTYKIEWICZ G., MAŃKA G., CIMANDER B., CHORĄŻRY M., Mutagenic Activity in Air-Borne Particulate Pollutants at Industrial District of Silesia, Bull. Pol. Acad. Scie. — Biol. Scie., 1985, 33, 7-16.
• [58] MOTYKIEWICZ G., MICHALSKA J., SZELIGA J., CJMANDER B., Mutagenic and clastogenic activity of direct-acting components from air pollutants of the Silesian industrial region, Mutation Res., 1988, 204, 289-296.
• [59] MOTYKIEWICZ G G., PENDZICH J., Genotoxic activity of atmospheric pollutants (in Polish), Biotechnologia, 1991, 13-14, 112-119.
• [60] MOTYKIEWICZ G., SZELIGA J., CIMANDER B., CHORĄŻRY M., Seasonal variations in mutagenic activity of air pollutants at an industrial district of Silesia, Mutation Res., 1989, 223, 243-251.
• [61] MOTYKIEWICZ G., SZELIGA J., SEEMAYER N.H., Phenotypic Transformation of Hamster Kidney Cells by Extracts of Airborne Pollutants from Upper Silesia, Bull. Pol. Acad. Scie. — Biol. Scie., 1995, 43, 99-103.
• [62] MURIN G., МICETA K., KNASMOLLER S., STEINKELLNER J., Bioindication of Radioactive- Contaminated Sites near Nuclear Power Plant .Iaslovskę Bohunice (in Slovak), 'Livot. Prostr., 1996, 30, 140-142.
• [63] NYLAND L., HAKALA E., SoRSA M., Application of a semi-automate SOS chromotest for measuring genotoxicities of complex environmental mixtures containing polycyclic aromatic hydrocarbons, Mutation Res., 1992, 276, 125-132.
• [64] OVERBO S., FJELDSTD P.E., GRZYBOWSKA E., KURE E.H., CHORĄŻRY M., HAUGEN A., Biological Monitoring of Polycyclic Aromatic Hydrocarbon Exposure in a ugly Polluted Area of Poland, Environ. Health Persp., 1995, 103, 838-843.
• [65] PITTS Jr. J.N., VAN CAUWENBERGIE K.A., GROSJEAN D., SCHMID J.P., FITZ D.R., BELSER Jr. W.L., KNUDSPN G.B., HYNDS P.M., Atmospheric Reactions of Polycyclic Aromatic Hydrocarbons: Facile Formation of Mutagenic Nitro Den vales, Science, 1978, 202, 515-519.
• [66] PRUUL R., NYLAND L., РELТONEN K., SORSA M., VEDEBAUM T., Environmental Genotoxicity in an Estonian Oil Shale Industrial Area, ATLA, 1992, 24, 419-422.
• [67] PYYSALO H., TUOMINEN J., WICKSTROM K., SKYTTA E., TIKKANEN L., SALOMAA S., SORSA M., NURMELA T., MATTILA T., POHJOLA V., Polycyclic organic material (РОМ) in urban air. Fractionation, chemical analysis and genotoxicity of particulate and vapour phases in an industrial town in Finland, Atmos. Environ., 1987, 21, 1167-1180.
• [68] De RAAT W.K., Genotoxicity of aerosol extracts. Some methodological aspects and the contribution of urban and industrial locations, Mutation Res., 1983, 116, 47-63.
• [69] De RAAT W.K., BAKKER G.L., MEIJERE F.A., Comparison of Filter Materials Used for Sampling of Mutagen and Polycyclic Aromatic Hydrocarbons in Ambient Airborne Particles, Atmos. Environ., 1990, 24А, 2875-2888.
• [70] De RAAT W.K., BOERS J.P., BAKKER G.L., De MEUERE F.A., HOOIMEIJER A., LOHMAN P.H.M., MORN G.R., Contribution of РАН and Some of Their Nitrated Derivatives to the Mutagenicity of Ambient Airborne Particles and Coal Fly Ash, Sci. Total. Environ., 1994, 153, 7-29.
• [71] De RAAT W.K., MEUERE F.A., The Mutagenicity of Ambient Air from Local Traffic and Distant Sources During Episodes with Moderate Photochemical Air Pollution, Sci. Total. Environ., 1988, 73,159-180.
• [72] REIMER P., Grounds of ecotoxicology (in Polish), Ekoinżynieria, Lublin, 1997.
• [73] ROEPSTORFF V., OSTENFELD N., AuTRUP H., Extracts of airborne particulates collected at different locations in the Copenhagen area induce the expressin of cytochrome P-4501А1, J. Toxicol. Environ. Health, 1990, 30, 225-237.
• [74] RISSI C., POLI P., BUSCINI A., CASSONI F., CARRANI S., De MUNARI E., Comparative investigations among meteorological conditions, air chemical-physical pollutants and airborne particulate mutagenicity: a long-term study (1990-1994) from a northern Italian town, Chemosphere, 1995, 30, 1829-1845.
• [75] SCARPATO R., Di MARINO F., STRAND A., CURT' A., CAMPAGNA R., LOPRIENO N., BARRAI I., BARALE R., Two years' air mutagenesis monitoring in a northwestern rural area of Italy with an industrial plant, Mutation Res., 1993, 319, 293-301.
• [76] SCHURER C.-C., MANOJLOVIC N., SEEMAYER N.H., Induction of sister chromatid exchange in human cells in vitro by the mutagenie effect of city smog, Mutation Res., 1980, 74, 164-165.
• [77] SCHURER C.-C., SEEMAYER N.H., MANOJLOVIC N., Induction of sister chromatid exchange in human lymphocytes in vitro by extracts of airborne particulate matter: comparison of samples from highly industrialized area followed up for several years, Mutation Res., 1983, 113, 347.
• [78] SEEMAYER N., Zelltransformation von Hamsternierenzellen in vitro inter dem Einflufś von atmospharischen Feinstaubextraken and dem Papavavirus 5V40, Staub-Reinhalt, Luft, 1978, 38, 254-258.
• [79] SEEMAYER N.H., HORNEERG C., Malignat transformation of syrian hamster kidney celles in vitro by interaction of airborne particulates and simian virus (SV-) 40, Toxicol. Lett., 1998, 96-97, 231-238.
• [80] SEEMAYER N.H., MANOJLOVIC N., SCH0RЕR C.-C., TOMINGAs R., Cell cultures as a tool for detection of cytotoxic, mutagenic and carcinogenic activity of airborne particulate matter, J. Aerosol Sci., 1984, 15, 426-430.
• [81] SEEMAYER N.H., SCHОRЕR C.-C., KONIG H., MANOJLOVIC N., Mutagenicity of airborne pollutants: induction of point mutations and sister chromatid exchanges in tissue culture cells, Mutation Res., 1985,147,134-135.
• [82] SEEMAYER N., MANOJLOVIC N., ToMlNGAS R., Die Wirkung von kanzerogenen Stollen, insbesondere von atmosphdrischen Feinstaubextraken, im Zelltransformationstest, Lufrverunreinigung durch polycyklische aromatische Kohlenwasserstofe-Erfassung and Bewertung, VDI-Berichte, 1980, 358, 285-292.
• [83] SZELIGA J., СНORР У M., CIMANDER B., Qualitative and quantitative chemical analysis of suspended matter extracts in the air of Silesia region (in Polish), Biotechnologia, 1991, 13-14, 102-111.
• [84] SZESZENIA-DABROWSKA M., Helth effects of environment pollution (in Polish), Bid ioteka Monitoringu Środowiska, PIGS, Warszawa 1994.
• [85] TOKIWA H., KITAMORI S., TAKAUASHI K., OHNISHI Y., Mutagenic and chemical assay extracts of airborne particulates, Mutation Res., 1980, 77, 99-108.
• [86] TOPINKA J., BINKOVA B., MRACKOVA G., BENES I., DEJMEK J., LENICEK J., SRAM R.J., DNA adducts in human placenta as related to air pollution and to GSTM1 genotype, Mutation Res., 1997, 390, 59-68.
• [87] TRACZEWSKA T.M., KOLWZAN B., CZARNY A., KUŚNIERZ M., PAWLACZYK-SZPILOWA M., Genotoxicity and cytotoxicity of atmospheric pollutants on the area of Wrocław City (in Polish), [in:] Environment degradationa and human health, V Krajowy Kongres Ekologiczny EKO-MED, Tarnów '98, Tarnowska Agencja Rozwoju Regionalnego SATarnów, 1998, 255-266.
• [88] TSAI J.-H., PENG B.-H., LEE D.-L., LEE Ch.-Ch., PAN characteristics and genotoxicity in the ambient air of a petrochemical industry complex, Environ. International, 1995, 21, 47-56.
• [89] WALKER R.D., CONNOR T.H., MACDONALD E.J., TRIEFF N.M., LEGATOR M.S., MacKENZIE K.W., DOBBINs J.G., Correlation of Mutagenic Assessment of Houston Air Particulate Extracts in Relation to Lung Cancer Mortality Rates, Environ. Res., 1982, 28, 303-312.
• [90] WASSERKORT R., IARTMANN A., WIDMER R.M., BURTSCNER I., Correlation between on-line РАН detection in airborne particle samples and their bacterial genotoxicity, Ecotoxicol. Environ, Safety, 1998, 40, 126-136.
• [91] WESTERHOLM R.N., АLME N J., Li H., RANNUC J.U., EGEBACK K.-E., GRAGG K., Chemical and Biological Characterization of Particulate-, Semivolatile-, and Gas-Phase-Associated Compounds in Diluted Heavy-Duty Diesel Exhausts: A Comparison of Three Different Semivolatile-Phase Samplers, Environ. Sci. Technol., 1991, 25, 332-338.
• [92] De WIEST F., RONDIA D., GOL-WINKLER R., GIELEN J., Mutagenic activity of non-volatile organic matter associated with suspended matter in urban air, Mutation Res., 1982, 104, 201-207.
• [93] WISE S.S., CHE$LER S.N., HILPERT L.R., MAY W.E., REBBERT R.E., VOGT C.R., NISHIOKA M.G., AUSTIN A., LEWTAS J., Quantification of polycyclic aromatic hydrocarbons and nitro-substituted polycyclic aromatic hydrocarbons and mutagenicity testing for the characterization of ambient air particulate matter, Environment. International, 1985, 11, 147-160.
• [94] ZWOŹDZIAK J., JADСZYК P., KUCHARCZYK J., Seasonal variability of the mutagenicity of airborne particles in the town center, J. Aerosol Sci., 2001, 32, 409-423.