Evaluation of thin-layer chromatography systems for analysis of amino acids in complex mixtures

• Autorzy: Vasta, J. D. , Cicchi, M. , Sherma, J. , Fried, B.
• Języki publikacji: EN

Abstrakty

EN

Different thin-layer chromatography (TLC) systems were evaluated for analysis of 21 biologically important essential and nonessential amino acids in complex mixtures such as biological tissues and fluids. Amino acids were visualized on the layers by derivatization with ninhydrin reagent, and R _F values were determined by slit-scanning densitometry. The five systems found to be most useful for analysis of amino acids were cellulose and silica gel high-performance TLC (HPTLC) plates developed with either 2-butanol-pyridine-glacial acetic acid-deionized water, 39:34:10:26, or 2-butanol-pyridine-25% ammonia-deionized water, 39:34:10:26, and ion exchange TLC plates developed with citrate buffer, pH 3.3. Using these five systems with ninhydrin detection, identification of all amino acids except for leucine and isoleucine in complex mixtures is possible, and quantification can be achieved if the amino acid to be quantified is well separated from adjacent components of the mixture. Example chromatograms are illustrated for separation and identification of amino acids in a snail tissue sample on a cellulose HPTLC plate.

Słowa kluczowe

EN

amino acids; thin-layer chromatography; TLC; biological samples; Biomphalaria glabrata

• Czasopismo: Acta Chromatographica
• Rocznik: 2009
• Tom: Vol. 21, no. 1
• Strony: 29-38
• Opis fizyczny: Bibliogr. 11 poz., rys., tab.

Twórcy

autor

Vasta, J. D.

autor

Cicchi, M.

autor

Sherma, J.

autor

Fried, B.

• Lafayette College Department of Chemistry Easton PA 18042-1782 USA Lafayette College Department of Biology Easton PA 18042-1782 USA,

Bibliografia

• [1] B.P Sleckman and J. Sherma, J. Liq. Chromatogr., 5 , 1051 (1982)
• [2] J. Sherma, B.P. Sleckman, and D.W. Armstrong, J. Liq. Chromatogr., 6 , 95 (1983)
• [3] E. Norfolk, S.H. Khan, B. Fried, and J. Sherma, J. Liq. Chromatogr., 17 , 1317 (1994)
• [4] R.A. Steiner, B. Fried, and J. Sherma, J. Liq. Chromatogr., 21 , 427 (1998)
• [5] E.L. Ponder, B. Fried, and J. Sherma, J. Parasitol., 90 , 665 (2004)
• [6] J. Pachuski, S.D. Wagner, B. Fried, and J. Sherma, Comp. Parasitol., 69 , 202 (2002)
• [7] C.F. Poole, The Essence of Chromatography, Elsevier, The Netherlands, pp. 536–538
• [8] [8] ProteoChrom Application sheet for HPTLC cellulose sheets, Cat. No. 1.05651.001, Merck, 64271, Darmstadt, Germany
• [9] [9] J.D. Vasta, B. Fried, and J. Sherma, J. Planar Chromatogr., 21 , 39 (2008)
• [10] P.A. Zani, J.L. Counihan, J.D. Vasta, and J. Sherma, J. Liq. Chromatogr. Related Technol., 31 , 1881 (2008)
• [11] S.R. Bandstra, J.L. Schneck, B. Fried, and J. Sherma, Acta Universitatis Cibiensis Seria F Chemia, 7 , 3 (2004).