HPLC and 13C NMR for analysis of transamidation in a dynamic combinatorial chemistry system

• Autorzy: Pałka, A. , Musioł, R. , Matlengiewicz, M. , Sajewicz, M. , Serafin, K. , Polański, J.
• Języki publikacji: EN

Abstrakty

EN

The permanent demand for novel drugs by the pharmaceutical industry results in a variety of novel methods for drug discovery. Combinatorial methods are a novel direction of drug research based on synthesis of large number of compounds in the hope of finding interesting drug-like molecules. In dynamic combinatorial chemistry (DCC) an enzyme is used to control the synthetic path and increase the proportion of the drug-like molecules in the reaction mixture. Because the mixture formed by the reaction is complex, analytical procedures limit possible application of the method. In the work discussed in this publication HPLC was coupled with 13C NMR for analysis of a DCC system. Transamidation controlled by use of Candida rugosa lipase was compared with the reaction without the enzyme. This enabled us to show that the enzyme-promoted reaction favoured the amine with the lowest nucleophilicity.

• Czasopismo: Acta Chromatographica
• Rocznik: 2006
• Tom: No. 17
• Strony: 314-319
• Opis fizyczny: Bibliogr. 11 poz., rys.

Twórcy

autor

Pałka, A.

autor

Musioł, R.

autor

Matlengiewicz, M.

autor

Sajewicz, M.

autor

Serafin, K.

autor

Polański, J.

• Institute of Chemistry, University of Silesia, Szkolna 9, 40-006 Katowice, Poland

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