Application of micelle-mediated extraction in preparation of body fluids for HPLC-DAD screening of acidic and neutral drugs

• Autorzy: Madej, K. , Persona, K. , Nizio, M.
• Języki publikacji: EN

Abstrakty

EN

Experimental conditions of cloud-point extraction (CPE) for the selected five acidic and neutral medicaments (salicylic acid, opipramol, carbamazepine, lorazepam, and alprazolam) in human plasma were studied and optimized. Separation and detection of the tested drugs were performed by the high-performance liquid chromatography with diode array detection (HPLC-DAD) method in an appropriate gradient mode using a column Nucleosil C8. Under the optimized conditions, main validation parameters were determined for all the compounds. The extraction yields (%) ranged from 54.12 to 82.17 with intra- and interday repeatability (RSD, %) 5.70–9.92 and 5.79–10.19, respectively. The detection limit was 0.5 μg mL^-1 for all the tested drugs with exception of salicylic acid (LOD = 2.5 μg mL^-1). The linearity of the proposed method was examined for the four drugs: opipramol, carbamazepine, lorazepam, and alprazolam in the concentration range of 0.5–2.0 μg mL^-1 (correlation coefficient r2 = 0.995–0.999) and for salicylic acid in the concentration range of 2.5–10.0 μg mL^-1 (correlation coefficient r2 = 0.993). The analytical parameters for the medicaments tested in whole blood were unsatisfactory, especially in terms of extraction recovery and repeatability, and application of the developed procedure for this biological matrix requires further study.

Słowa kluczowe

EN

micelle-mediated extraction; cloud-point extraction; screening drug analysis; acidic/neutral medicaments; body fluids

• Czasopismo: Acta Chromatographica
• Rocznik: 2013
• Tom: Vol. 25, no. 1
• Strony: 97--110
• Opis fizyczny: Bibliogr. 16 poz., rys., tab.

Twórcy

autor

Madej, K.

• Jagiellonian University Department of Analytical Chemistry, Faculty of Chemistry Ingardena St. 3 30-060 Krakow Poland,

autor

Persona, K.

• Jagiellonian University Department of Analytical Chemistry, Faculty of Chemistry Ingardena St. 3 30-060 Krakow Poland

autor

Nizio, M.

• Jagiellonian University Department of Analytical Chemistry, Faculty of Chemistry Ingardena St. 3 30-060 Krakow Poland

Bibliografia

• [1] C. Moffat, M. D. Osselton and B. Widdop (eds), Clarke’s Analysis of Drugs and Poisons, Pharmaceutical Press, London, 2004, pp. 3–36
• [2] K. Madej, Trends Anal. Chem., 28, 436 (2009)
• [3] Z.-M. Zhou, D.-Y. Zhao, J. Wang, W.-J. Zhao and M.-M. Yang, J. Chromatogr. A, 1216, 30 (2009)
• [4] Ch. Ch. Wang, M. O. Luconi, A. N. Masi and L. Fernández, Talanta, 72, 1779 (2007)
• [5] H. W., G.-Y. Zhao and L.-M. Du, Spectrochim. Acta A, 75, 1624 (2010)
• [6] H. Filik, İ. Şener, S. D. Cekiç, E. Kiliç and R. Apak, Chem. Pharm. Bull., 54, 891 (2006)
• [7] A. B. Tabrizi, Bull. Korean Chem. Soc., 27, 1604 (2006).
• [8] H. Zhang and H.-K. Choi, Anal. Bioanal. Chem., 392, 947 (2008)
• [9] A. Ohashi, M. Ogiwara, R. Kieda, H. Okada and K. Ohashi, Anal. Sci., 20, 1353 (2004).
• [10] F. Han, R. Yin, X.-l. Shi, Q. Jia, H.-z. Liu, H.-m. Yao, L. Xu and S.-m. Li, J. Chromatogr. B, 868, 64 (2008)
• [11] W. Liu, K. Bi, X. Liu, J. Zhao and X. Chen, Chromatographia, 69, 837 (2009)
• [12] J. Zhou, P. Zeng, H. H. Tu and F. Q. Wang, J. Sep. Sci, 34, 160 (2011)
• [13] E. K. Paleologos, D. L. Giokas and M. I. Karayannis, Trends Anal. Chem., 24, 426 (2005).
• [14] A. Tong, Y. Wu, S. Tan, L. Li, Y. Akama and S. Tanaka, Anal. Chim. Acta, 369, 11 (1998)
• [15] The Bulletin of the International Association of Forensic Toxicologists, 26, Supplement (1996)
• [16] Ch. L. Winek, W. W. Wahba, Ch. L. Winek Jr. and T. Winek-Balzer, 122, 107 (2001)