Determination of nicotine and three minor alkaloids in tobacco by hydrophilic interaction chromatography-tandem mass spectrometry

• Autorzy: Taujenis, L. , Olšauskaitė, V. , Padarauskas, A.
• Języki publikacji: EN

Abstrakty

EN

A reliable, sensitive and rapid method for determination of nicotine and three minor alkaloids (cotinine, anabasine and nornicotine) in tobacco by ultra-high performance liquid chromatography in hydrophilic interaction chromatography mode coupled with tandem mass spectrometry (HILIC-MS/MS) has been established. HILIC separation was performed on a BEH HILIC column using isocratic elution at 0.5 mL/min with acetonitrile:water (85:15, v/v) mobile phase containing 5 mmol/L ammonium acetate (pH 5.00). Separated analytes were determined by electrospray ionization MS/MS in the positive ion mode using multiple reaction monitoring. Alkaloids from tobacco were extracted in an ultrasonic bath for 10 min with acetonitrile:water mixture (8:2, v/v) containing 5 mmol/L ammonium acetate (pH 5.00). Limits of quantification were 10 μg/g for cotinine, 20 μg/g for anabasine and nornicotine, and 30 μg/g for nicotine. Mean recoveries from tobacco ranged between 94.8% and 104.1% for different analytes with relative standard deviations within 5%. The performance of the proposed method was tested for the extraction and determination of the four alkaloids in cigarette tobacco fillers, and satisfactory results were achieved.

Słowa kluczowe

EN

hydrophilic interaction chromatography; tandem mass spectrometry; nicotine; minor alkaloids; tobacco

• Czasopismo: Acta Chromatographica
• Rocznik: 2015
• Tom: Vol. 27, no. 2
• Strony: 373--385
• Opis fizyczny: Bibliogr. 32 poz., rys., tab.

Twórcy

autor

Taujenis, L.

• Vilnius University Department of Analytical and Environmental Chemistry Naugarduko 24 LT-03225 Vilnius Lithuania

autor

Olšauskaitė, V.

• Vilnius University Department of Analytical and Environmental Chemistry Naugarduko 24 LT-03225 Vilnius Lithuania

autor

Padarauskas, A.

• Vilnius University Department of Analytical and Environmental Chemistry Naugarduko 24 LT-03225 Vilnius Lithuania,

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