Validated HPTLC method for quantification of epicatechin in extracts of leaves of Cassia fistula Linn.

• Autorzy: Nagore, D. H. , Ghosh, V. K. , Patil, M. J. , Wahile, A. M.
• Języki publikacji: EN

Abstrakty

EN

This paper describes a new, simple, precise, and accurate HPTLC method for quantification of (−)-epicatechin in the leaves of Cassia fistula . The leaves were separately extracted with methanol and water by both maceration and hot extraction (Soxhlet apparatus). Chromatographic separation of the drug was performed on aluminium foil silica gel 60 F 254 plates with toluene-ethyl acetate-formic acid-methanol 20:12:4:4 ( v / v ) as mobile phase. Densitometric evaluation of the separated zone was performed at 280 nm. Epicatechin in the extract was satisfactorily resolved with R F 0.22 ± 0.02. The accuracy and reliability of the method were assessed by evaluation of linearity (200–800 ng per band), precision (method precision RSD 1.42% and instrumental precision RSD 1.12%), accuracy (98.12%), and specificity in accordance with ICH guidelines.

Słowa kluczowe

EN

HPTLC; validation; epicatechin; Cassia fistula

• Czasopismo: Acta Chromatographica
• Rocznik: 2010
• Tom: Vol. 22, no. 2
• Strony: 259--265
• Opis fizyczny: Bibliogr. 10 poz., rys., tab.

Twórcy

autor

Nagore, D. H.

• Tulip Lab Pvt. Ltd. F-20/21 MIDC Ranjangaon, Tal-Shirur Pune 412220 India,

autor

Ghosh, V. K.

• Tulip Lab Pvt. Ltd. F-20/21 MIDC Ranjangaon, Tal-Shirur Pune 412220 India

autor

Patil, M. J.

• Marathwada Mitra Mandal College of Pharmacy Pune India

autor

Wahile, A. M.

• Natural Remedies Bangalore India

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