Development and validation of high-performance thin-layer chromatography method for determination of vicine in herbal extract and formulation

• Autorzy: Bedi, R. S. , Gauttam, V. K. , Kalia, A. N.
• Języki publikacji: EN

Abstrakty

EN

A simple, selective, precise, and reproducible high-performance thin-layer chromatography (HPTLC) method for the analysis of vicine in herbal extracts and formulations containing Momordica charantia was developed. The study was performed on aluminum-based pre-coated TLC plates (silica gel 60F254). The chromatograms of samples were developed in twin trough glass chamber pre-saturated with mobile phase comprised of ethyl acetate-methanol-water-formic acid (7.5:3:1:0.1, v/v/v/v) at room temperature (25 ± 2°C). The densitometric analysis was carried out in absorbance mode at 278 nm. The optimized mobile phase showed a compact spot of vicine (RF = 0.31 ± 0.02) from the other constituents present in the samples. The linear regression analysis data for the calibration plots showed good linearity (r2 = 0.9990) with respect to peak area in the range of 200–1400 ng spot ^-1. The method was validated as per International Conference on Harmonization (ICH) guidelines. The limits of detection and quantification (50 and 150 ng spot−1, respectively) were also established. The proposed method has shown the excellent recovery (98.01–99.33%), which supports the suitability of the method for the analysis. Statistical analysis of the obtained data showed the selectivity of the proposed method for vicine estimation in the herbal samples.

Słowa kluczowe

EN

herbal extract; Momordica charantia; validation; vicine

• Czasopismo: Acta Chromatographica
• Rocznik: 2014
• Tom: Vol. 26, no. 3
• Strony: 551--562
• Opis fizyczny: Bibliogr. 24 poz., rys., tab.

Twórcy

autor

Bedi, R. S.

• ISF College of Pharmacy Department of Pharmacognosy Moga 142001 India

autor

Gauttam, V. K.

• ISF College of Pharmacy Department of Pharmacognosy Moga 142001 India

autor

Kalia, A. N.

• ISF College of Pharmacy Department of Pharmacognosy Moga 142001 India,

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