Determination of icariin in bushenqiangshen capsule in high-performance liquid chromatography with fluorescence detection by precolumn chelation with aluminum

• Autorzy: Yang, G. J. , Lv, L.
• Języki publikacji: EN

Abstrakty

EN

A simple and sensitive method of high-performance liquid chromatography with fluorescence detection (HPLC-FLD) was developed for the determination of icariin in capsules by precolumn chelation with aluminum. In order to obtain a stable fluorescence signal, the reaction conditions of the fluorescent chelation complex between icariin and aluminum were investigated in detail. Chromatography was carried out on an Agilent Zorbax Extend C18 column (150 mm × 4.6 mm, 5.0 μm) using methanol as mobile phase at a flow rate of 1.0 mL min^-1. The excitation and emission wavelengths were set at 430 and 480 nm, respectively. At optimum conditions, the calibration curve was linear in the concentration range from 0.010 to 100.0 μg mL^-1 with the limit of detection of 3.5 ng mL^-1 (S/N = 3). A comprehensive method was validated for precision and accuracy. The method described here has been successfully applied for the determination of the icariin content in a capsule with satisfactory results.

Słowa kluczowe

EN

high-performance liquid chromatography; fluorescence detection; icariin; AlCl3; capsule

• Czasopismo: Acta Chromatographica
• Rocznik: 2012
• Tom: Vol. 24, no. 2
• Strony: 229--239
• Opis fizyczny: Bibliogr. 11 poz., rys., tab.

Twórcy

autor

Yang, G. J.

• China Pharmaceutical University School of Pharmacy Nanjing 210009 P. R. China,

autor

Lv, L.

• Yangzhou University College of Chemistry and Chemical Engineering Yangzhou 225002 P. R. China

Bibliografia

• [1] D.Y. Zhu, L.H. Qu, X.N. Zhang, and Y.J. Lou, Eur. J. Pharmacol., 514, 99 (2005)
• [2] Q.Z. Du, M. Xia, and Y. Ito, J. Chromatogr. A, 962, 239 (2002)
• [3] J.Q. Zhang, X.M. Wang, Z.Q. Lu, H.L. Huang, G.T. Chen, R.X. Liu, K.S. Bi, and D.A. Guo, Chromatographia, 66, 267 (2007)
• [4] J. Liu and Y.J. Lou, J. Pharm. Biomed. Anal., 36, 365 (2004)
• [5] Y.P. Zhang, W. Xu, N. Li, H.Y. Li, Z.Y. Shen, X.M. Zhang, M. Yang, W.D. Zhang, and C. Zhang, Chromatographia, 68, 245 (2008)
• [6] Y. Tu, L. Zhu, G.J. Wang, L.H. Zhao, and B.R. Xiang, Chromatographia, 67, 591 (2008)
• [7] W. Xu, Y.P. Zhang, M. Yang, Z.Y. Shen, X.M. Zhang, W.D. Zhang, and H.Y. Li, J. Pharm. Biomed. Anal., 45, 667 (2007)
• [8] M. Liu, H.P. Liu, X.M. Lu, C. Li, Z.L. Xiong, and F.M. Li, J. Chromatogr. B, 860, 113 (2007)
• [9] G.F. Deng, D.L. Wang, M.X. Meng, F. Hu, and T.W. Yao, J. Chromatogr. B, 877, 2113 (2009)
• [10] P.C.H. Hollman, J.M.P. Trijp, and M.N.C.P. Buysman, Anal. Chem., 68, 3511 (1996)
• [11] R.H. Wang and N.Q. Li, Chinese J. Anal. Chem., 22, 808 (1994)