Prospects for the application of aptamer based assay platforms in pathogen detection

• Autorzy: Banu, Kauser , Mondal, Bhairab , Rai, Bhawana , Monica, N. , Hanumegowda, Raju
• Języki publikacji: EN

Abstrakty

EN

Aptamer-based diagnostics platforms for animal, human, plant and environmental pathogens are gaining importance as they are rapid, user-friendly, sensitive and selective. However, most of the aptamer-based platforms have not yet become commercially available. The increasing number of publications signifies the applications of aptamer-based platform and their potential. Herein, the present review is to describe, a brief overview of the development of various aptamer-based platforms and their applicability for the sensitive detection of pathogens. In this review, several aptamer-based platforms such as Enzyme linked immunosorbent assay (ELISA)-like assay, Apta blot, Apta Polymerase Chain Reaction (PCR), Apta array, Aptamer-based Lateral Flow Assays (LFA), Aptamer based fluorescence assay, Flowcytometry-based assay, Apta affinity chromatography, microfluidics-based platforms, and various aptasensor have been discussed. Most of the platforms are highlighted will encourage researchers to focus on developing pathogen detection platform for various applications.

Słowa kluczowe

PL

aptamer; metoda SELEX; cytometria przepływowa

EN

aptamer; SELEX; flow cytometry

• Czasopismo: Biocybernetics and Biomedical Engineering
• Rocznik: 2022
• Tom: Vol. 42, no. 3
• Strony: 934--949
• Opis fizyczny: Bibliogr. 114 poz., rys., tab.

Twórcy

autor

Banu, Kauser

• Shankaranarayana Life Sciences LLP, Bengaluru, Karnataka, India

autor

Mondal, Bhairab

• 144-C/1, Kirloskar Road, Bommasandra Industrial Estate, Hebbagodi, Bengaluru, Karnataka 560100, India,

autor

Rai, Bhawana

• Shankaranarayana Life Sciences LLP, Bengaluru, Karnataka, India

autor

Monica, N.

• Shankaranarayana Life Sciences LLP, Bengaluru, Karnataka, India

autor

Hanumegowda, Raju

• Shankaranarayana Life Sciences LLP, Bengaluru, Karnataka, India
• Department of Biotechnology, RVCE, Bengaluru, Karnataka, India

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Identyfikatory

DOI

10.1016/j.bbe.2022.07.005