The neuroprotective efFect exerted by oligodendroglial progenitors on ischemically impaired hippocampal slices

• Autorzy: Sypecka J. , Sarnowska A.
• Języki publikacji: EN

Abstrakty

EN

The role of oligodendrocytes is supposed to go beyond the process of myelinating nerve fibers in the central nervous system.  Pre-clinical studies based on oligodendrocyte progenitor cell (OPCs) therapies revealed a significant behavioral improvement in spite of failure in the remyelination process in animal models of demyelinating diseases. To address the issue of potential trophic support conferred by OPC to diseased nervous tissue, co-culture experiments with neonatal rat OPCs and organotypic hippocampal slices were designed. For this purpose, rat organotypic hippocampal slices were exposed to a brief oxygen and glucose deprivation (OGD) which allowed mimicking an ischemic injury in vitro. Soon after the OGD procedure, the hippocampal slices were co-cultured together with differentiating oligodendroglial progenitors. Cell survival and their proliferation rates in the injured slices were estimated by immunohistochemical methods. After acknowledging the beneficial influence of the neighboring oligodendrocytes on the injured tissue, a set of molecular and biochemical experiments were carried out with the aim of determining the mechanism(s) of the observed profound neuroprotective effect. The results of both molecular studies and functional assays revealed that the oligodendrocyte-derived BDNF is the major factor promoting neuronal survival in OGD-subjected hippocampal slices, while SCF and IL-10 strongly promote the cell proliferation. Among the newly-born cells, neuroblasts and microglia could most frequently be found pointing to both the neuroprotective and neuroimmunomodulatory role of differentiating oligodendrocytes. In conclusion, the presented study revealed that oligodendrocytes are able to secret BDNF and other active factors, thus providing trophic support for other neural cells. The presented study confirms the hypothesis concerning the complex functions of oligodendrocytes in the nervous tissue. Supported by MMRC statutory funds.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S21-S22

Twórcy

autor

Sypecka J.

• NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Sarnowska A.

• NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland