Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl

PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2019 | 41 | 07 |
Tytuł artykułu

Lutein content in petals and leaves of marigold and analysis of lutein synthesis gene expression

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Lutein is a bioactive natural carotenoid that is a member of the oxygen-containing carotenoids, and marigolds are the main raw material for the extraction of lutein. The lutein content in the leaves and petals of eight varieties of marigold including two inbred lines and six nuclear male sterile AB lines was measured using high-performance liquid chromatography (HPLC). The results showed that lutein was mainly stored in the petals of marigold, which had a lutein content 20 times higher than that in the leaves. Additionally, the lutein content in male parent 3 was the highest among the eight varieties of marigold in both petals and leaves. Correlation analysis showed that the expression level of the LCYE gene in the petals was positively correlated with the lutein content, with a correlation coefficient of 0.82, and the expression level of the LCYB gene in the leaves was positively correlated with the lutein content, with correlation a coefficient of 0.77. This study laid the foundation for the evaluation of parental resources and quality breeding of pigment marigold.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
41
Numer
07
Opis fizyczny
Article 128 [11p.], fig.,ref.
Twórcy
autor
  • Beijing Key Laboratory of Ornamental Germplasm Innovation and Molecular Breeding, China National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, China
  • Beijing Agro‑Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China
autor
  • Beijing Agro‑Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China
autor
  • Beijing Agro‑Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China
autor
  • Beijing Agro‑Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China
autor
  • Beijing Key Laboratory of Ornamental Germplasm Innovation and Molecular Breeding, China National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, China
Bibliografia
  • Alquezar B, Zacarias L, Rodrigo MJ (2009) Molecular and functional characterization of a novel chromoplast-specific lycopene beta-cyclase from Citrus and its relation to lycopene accumulation. J Exp Bot 60(6):1783–1797
  • Barnes HT (2004) Formulating beverages for healthy eyes and skin. Soft Drinks Manag Int 25(6):27
  • Breitenbach J, Sandmann G (2005) ζ-Carotene cis isomers as products and substrates in the plant poly-cis carotenoid biosynthetic pathway to lycopene. Planta 220(5):785–793
  • Clotault J, Peltier D, Berruyer R, Thomas M, Briard M, Geoffriau E (2008) Expression of carotenoid biosynthesis genes during carrot root development. J Exp Bot 59:3563–3573
  • Cunningham FXJ, Gantt E (2001) One ring or two? Determination of ring number in carotenoids by lycopene epsilo-cyclases. Proc Natl Acad Sci USA 98(5):2905–2910
  • Cunningham FX Jr, Pogson B, Sun Z et al (1996) Functional analysis of the beta and epsilon lycopene cyclase enzymes of Arabidopsis reveals a mechanism for control of cyclic carotenoid formation. Plant Cell 8:1613–1626
  • Del Villar-Martínez AA, García-Saucedo PA, Carabez-Trejo A, Cruz-Hernández A, Paredes-Lópeza O (2005) Carotenogenic gene expression and ultrastructural changes during development in marigold. J Plant Physiol 162(9):1046–1056
  • Devitt LC, Fanning K, Dietzgen RG et al (2010) Isolation and functional characterization of a lycopene beta-cyclase gene that controls fruit colour of papaya (Carica papaya L.). J Exp Bot 61(1):33–39
  • Hirschberg J (2001) Carotenoid biosynthesis in flowering plants. Curr Opin Plant Biol 4(3):210–218
  • Johnson EJ (2002) The role of carotenoids in human health. Nutr Clin Care 5(2):56–65
  • Li F, Vallabhaneni R, Yu J, Rocheford T, Wurtzel ET (2008) The maize phytoene synthase gene family: overlapping roles for carotenogenesis in endosperm, photomorphogenesis, and the thermal stress tolerance. Plant Physiol 147:1334–1346
  • Li Z, Ahn TK, Avenson TJ et al (2009) Lutein accumulation in the absence of zeaxanthin restores nonphotochemical quenching in the Arabidopsis thaliana npq1 mutant. Plant Cell 21:1798–1812
  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods 25:402–408
  • Lu QY, Hung JC, Heber D (2001) Inverse associations between plasma lycopene and other carotenoids and prostate cancer. Cancer Epidemiol Biomarkers Prev 10(7):749–756
  • Moehs CP, Tian L, Osteryoung KW, DellaPenna D (2004) Analysis of carotenoid biosynthetic gene expression during marigold petal development. Plant Mol Biol 45(3):281–293
  • Park H, Kreunen SS, Cuttriss AJ, DellaPenna D, Pogson BJ (2002) Identification of the carotene isomerase provides insight into carotenoid biosynthesis, prolamellar body formation and photomorphogenesis. Plant Cell 14:321–332
  • Pecker I, Gabbay R, Cunningham FX, Hirschberg J (1996) Cloning and characterization of the cDNA for lycopene beta-cyclase from tomato reveals decrease in its expression during fruit ripening. Plant Mol Biol 30:807–819
  • Pogson BJ, Rissler HM (2000) Genetic manipulation of carotenoid biosynthesis and photoprotection. Philos Trans R Soc Lond B Biol Sci 355(1402):1395–1403
  • Pogson B, McDonald KA, Truong M, Britton G, DellaPenna D (1996) Arabidopsis carotenoid mutants demonstrate that lutein is not essential for photosynthesis in higher plants. Plant Cell 8:1627–1639
  • Ramos A, Coesel S, Marques A (2008) Isolation and characterization of a stress-inducible Dunaliella salina Lcy-beta gene encoding a functional lycopene beta-cyclase. Appl Microbiol Biotechnol 79(5):819–828
  • Subaio A, Morita N (2001) No effect of esterification with fatty acid on antioxidant activity of lutein. Food Res Int 34(40):315–320
  • Tanaka Y, Sasaki N, Ohmiya A (2008) Biosynthesis of plant pigments: anthocyanins, betalains and carotenoids. Plant J 54(4):733–749
  • Tian T, Musetti V, Kim J, Magallanes-Lundback M, DellaPenna D (2004) The Arabidopsis LUT1 locus encodesa member of the cytochrome P450 family that is required for carotenoid epsilon-ring hydroxylation activity. Proc Natl Acad Sci USA 101(1):402–407
  • Trosko JE (2003) The role of stem cells and gap junctional intercellular communication in carcinogenesis. J Biochem Mol Biol 36(1):43–48
  • Wang C, Li DJ, Song JF, Liu CQ (2010) Extraction and saponification of trans-lutein from marigold flowers. Food Sci 31(24):95–101
  • Wang Y, Li J, Yang J, Xia RX (2011) Expression of lycopene cyclase gene and their regulation on downstream carotenoids during fruit maturation of Guoqing No. 1 Satsuma mandarin and Cara Cara navel orange. Sci Hortic 127(3):267–274
  • Wang H, Ou C-G, Zhuang F-Y et al (2014) Relationship of carotenoid accumulation and transcript of main genes in carotenoid biosynthesis in carrot. Acta Hortic Sin 41(12):2513–2520
  • Yeum K-J, Aldini G, Chung H-Y, Krinsky N, Russell RM (2003) The activities of antioxidant nutrients in human plasma depend on the localization of attacking radical species. J Nutr 133(8):2688–2691
  • Zhao W, Zhang JQ, Qiao XS, Yu H, Han YH (2001) The determination of carotenoids in apple peel by HPLC. J Fruit Sci 18(2):95–97
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-f9eabde5-8899-45b1-b456-14a13247cc9b
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.