The influence of BDNF on human umbilical cord blood stem/progenitor cells: Implications for stem cell-based therapy of neurodegenerative disorders

• Autorzy: Paczkowska E. , Luczkowska K. , Piecyk K. , Roginska D. , Pius-Sadowska E. , Ustianowski P. , Cesarska E. , Dolegowska B. , Celewicz Z. , Machalinski B.
• Języki publikacji: EN

Abstrakty

EN

Umbilical cord blood (UCB)-derived stem/progenitor cells (SPCs) have demonstrated the potential to improve neurologic function in different experimental models. SPCs can survive after transplantation in the neural microenvironment and induce neuroprotection, endogenous neurogenesis by secreting a broad repertoire of trophic and immunomodulatory cytokines. In this study, the influence of brain-derived neurotrophic factor (BDNF) pre-treatment was comprehensively evaluated in a UCB-derived lineage-negative (Lin-) SPC population. UCB-derived Lin- cells were evaluated with respect to the expression of i) neuronal markers using immunofluorescence staining and ii) specific (TrkB) receptors for BDNF using flow cytometry. Next, after BDNF pre-treatment, Lin- cells were extensively assessed with respect to apoptosis using Western blotting and proliferation via BrdU incorporation. Furthermore, NT-3 expression levels in Lin- cells using RQ PCR and antioxidative enzyme activities were assessed. We demonstrated neuronal markers as well as TrkB expression in Lin- cells and the activation of the TrkB receptor by BDNF. BDNF pre-treatment diminished apoptosis in Lin- cells and influenced the proliferation of these cells. We observed significant changes in antioxidants as well as in the increased expression of NT-3 in Lin- cells following BDNF exposure. Complex global miRNA and mRNA profiling analyses using microarray technology and GSEA revealed the differential regulation of genes involved in the proliferation, gene expression, biosynthetic processes, translation, and protein targeting. Our results support the hypothesis that pre-treatment of stem/progenitor cells could be beneficial and may be used as an auxiliary strategy for improving the properties of SPCs.

Słowa kluczowe

EN

BDNF zob.brain derived neurotrophic factor; brain derived neurotrophic factor; man; umbilical cord blood; progenitor cell; stem cell; therapy; neurodegenerative disorder

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: 2
• Opis fizyczny: p.172-191,fig.,ref.

Twórcy

autor

Paczkowska E.

• Department of General Pathology, Pomeranian Medical University, Szczecin, Poland

autor

Luczkowska K.

• Department of General Pathology, Pomeranian Medical University, Szczecin, Poland

autor

Piecyk K.

• Department of General Pathology, Pomeranian Medical University, Szczecin, Poland

autor

Roginska D.

• Department of General Pathology, Pomeranian Medical University, Szczecin, Poland

autor

Pius-Sadowska E.

• Department of General Pathology, Pomeranian Medical University, Szczecin, Poland

autor

Ustianowski P.

• Department of Feto-Maternal Medicine and Gynecology, Pomeranian Medical University, Szczecin, Poland

autor

Cesarska E.

• Department of Laboratory Diagnostics and Molecular Medicine, Pomeranian Medical University, Szczecin, Poland

autor

Dolegowska B.

• Department of Laboratory Diagnostics and Molecular Medicine, Pomeranian Medical University, Szczecin, Poland

autor

Celewicz Z.

• Department of Feto-Maternal Medicine and Gynecology, Pomeranian Medical University, Szczecin, Poland

autor

Machalinski B.

• Department of General Pathology, Pomeranian Medical University, Szczecin, Poland

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