Cryopreservation of pharmaceutically important orchid Dendrobium chrysanthum Wall. ex Lindl. using vitrification based method

• Autorzy: Mohanty P. , Das M. C. , Kumaria S. , Tandon P.
• Języki publikacji: EN

Abstrakty

EN

Our present study constitutes the successful and efficient protocol for cryopreservation of Dendrobium chrysanthum. D. chrysanthum Wall. ex Lindl. is a pharmaceutically valuable, ornamental epiphytic orchid of temperate and subtropical regions. On account of excellent herbal medicinal value and horticultural importance, D. chrysanthum is becoming rare due to over exploitation. For long-term conservation of this orchid, protocorm-like bodies of D. chrysanthum were excised and used for cryopreservation by encapsulation–vitrification. In this cryogenic procedure, PLBs were initially osmoprotected with a mixture of 0.4 M sucrose and 2 M glycerol, incorporated in the encapsulation matrix (comprising of 3 % (w/v) sodium alginate and 0.1 M CaCl2). Encapsulated protocorm-like bodies (PLBs) were then precultured on MS liquid medium supplemented with different concentrations of sucrose (0.06, 0.3, 0.5, 0.7 M), and loaded in a loading solution (comprised of 2 M glycerol and 0.4 M sucrose) for different duration to make the precultured PLBs tolerant to plant vitrification solution 2 (PVS2). Subsequently, the PLBs were subjected to PVS2 (Sakai et al. 1990) treatment at different time of exposure (minutes) and temperatures (0 C and 25 C). Encapsulated–vitrified PLBs were plunged directly into liquid nitrogen and stored for 1 h. Optimum result (survival 63.2 % and regrowth 59.9 %) was obtained when the beads treated with loading solution for 80 min followed by PVS2 treatment for 100 min. Regenerated plants showed normal morphology as that of control plants.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 04
• Opis fizyczny: p.1373-1379,fig.,ref.

Twórcy

autor

Mohanty P.

• Plant Biotechnology Laboratory, Centre for Advanced Studies in Botany North Eastern Hill University, Shillong, India

autor

Das M. C.

• Plant Biotechnology Laboratory, Centre for Advanced Studies in Botany North Eastern Hill University, Shillong, India

autor

Kumaria S.

• Plant Biotechnology Laboratory, Centre for Advanced Studies in Botany North Eastern Hill University, Shillong, India

autor

Tandon P.

• Plant Biotechnology Laboratory, Centre for Advanced Studies in Botany North Eastern Hill University, Shillong, India

Bibliografia

• Bajaj YPS (1995) Cryopreservation of plant cell, tissue organ culture for the conservation of germplasm and biodiversity. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 32., Cryopreservation of plant germplasm ISpringer, Berlin, pp 3–47
• Bouafia S, Jelti N, Lairy G, Blanc A, Bonnel E, Dereuddre J (1996) Cryopreservation of potato shoots tips by encapsulation–dehydration. Potato Research 39:69–78
• Burritt DJ (2008) Efficient cryopreservation of adventitious shoots of Begonia xerythrophylla using encapsulation–dehydration requires pretreatment with both ABA and proline. Plant Cell Tissue Organ Cult 95:209–215
• Fahy GM, McFarlane DR, Angell CA, Meryman HT (1984) Vitrification as an approach to cryopreservation. Cryobiology 21:407–426
• Gale S, John A, Benson EE (2007) Cryopreservation of Picea sitchensis (Sitka spruce) embryogenic suspensor masses. Cryo Letters 28:225–239
• Hazubska-Przbyl T, Chmielarz P, Michalak M, Bojarczuk K (2010) Cryopreservation of embryogenic tissues of Picea omorika (Serbian spruce). Plant Cell Tissue Organ Cult 102:35–44
• Helliot B, Panis B, Swennen R, Lepoivre P, Frison E (2002) Cryopreservation for the elimination of cucumber mosaic or banana streak viruses from banana (Musa spp.). Plant Cell Rep 20:1117–1122
• Hirai D, Sakai A (2001) Recovery growth of plants cryopreserved by encapsulation–vitrification. Bull Hokkaido Prefect Agric Exp Station 80:55–64
• Hong PI, Chen JT, Chang WC (2008) Plant regeneration via protocorm-like body formation and shoot multiplication from seed-derived callus of a maudiae type slipper orchid. Acta Physiol Plant 30:755–759
• Hong S, Yin M, Shao X, Wang A, Xu W (2009) Cryopreservation of embryogenic callus of Dioscorea bulbifera by vitrification. Cryo Letters 30:64–75
• Hua YM, Hong HS (2010) A simple cryopreservation protocol of Dioscorea bulbifera L. embryogenic calli by encapsulation–vitrification. Plant Cell Tissue Organ Cult 102:35–44
• Ishikawa K, Harata K, Mii M, Sakai A, Yoshimatsu K, Shimomura K (1997) Cryopreservation of a Japanese terrestrial orchid (Bletilla striata) by vitrification. Plant Cell Rep 16:754–757
• Maneerattanarungroj P, Bunnag S, Monthatong M (2007) In vitro conservation of Cleisostoma areitinum (Rchb.f.) Garay, rare Thai orchid species by an encapsulation-dehydration method. Asian J Plant Sci 6:1235–1240
• Moges AD, Karam NS, Shibli RA (2004) Cryopreservation of African violet (Saintapulia ionantha Wendl.) shoot tips. In Vitro Cell Develop Biol-Plant 40:389–398
• Mohanty P, Das MC, Kumaria S, Tandon P (2012) High-efficiency cryopreservation of the medicinal orchid Dendrobium nobile Lindl. Plant Cell Tiss Organ Cult. doi:10.1007/s11240-011-0095-4
• Nishizawa S, Sakai A, Amano Y, Matsuzawa T (1993) Cryopreservation of asparagus (Asparagus officinalis L.) embryogenic suspension cells and subsequent plant regeneration by vitrification. Plant Sci 91:67–73
• Poobathy R, Nair H, Subramaniam S (2009) Optimisation of encapsulation-dehydration protocol for the orchid hybrid Ascocenda ‘princess mikasa’. Adv Environ Biol 3:69–83
• Pornchuti W, Thammasiri K (2008) Cryopreservation of protocorms of Dendrobium virgineum Rchb.f. Acta Hortic 788:63–68
• Sakai A, Kobayashi S, Oyiyama (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. Var. brasiliensis Tanka) by vitrification. Plant Cell Rep 9:30–33
• Salaj T, Matusˇıkova0 I, Fra0terova0 L, Pirsˇelova0 B, Salaj J (2011) Regrowth of embryogenic tissues of Pinus nigra following cryopreservation. Plant Cell Tissue Organ Cult 106:55–61
• Sharaf SA, Shibli RA, Kasrawi MA, Baghdadi SH (2012) Cryopreservation of wild Shih (Artemisia herba-alba Asso.) shoot-tips by encapsulation-dehydration and encapsulation–vitrification. Plant Cell Tissue Organ Cult 108:437–444. doi:10.1007/s11240-011-0054-0
• Sheelavantmath SS, Murthy HN, Pyati AN, Ashok Kumar HG, Ravishankar BV (2000) In vitro propagation of the endangered orchid, Geodorum densiflorum (Lam.) Schltr. through rhizome section culture. Plant Cell Tissue Organ Cult 60:151–154
• Shibli RA, Shatnawi MA, Subaih W, Ajlouni MM (2006) In vitro conservation and cryopreservation of plant genetic resources: a review. World J Agri Sci 2:372–382
• Thammasiri K (2000) Cryopreservation of seeds of a Thai orchid by vitrification. Cryo Letters 21:237–244
• Toshikazu M, Akira S, Kazuto Y (1995) Cryopreservation of in vitro grown apical meristems of lily by vitrification. Plant Cell, Tissue Organ Cult 41:237–241
• Touchell DH, Chiang VL, Tsai CJ (2002) Cryopreservation of embryogenic cultures of Picea mariana (black spruce) using vitrification. Plant Cell Rep 21:118–124
• Tsai SF, Yeh SD, Chan CF, Liaw SL (2009) High-efficiency vitrification protocols for cryopreservation of in vitro grown shoot tips of transgenic papaya lines. Plant Cell Tissue Organ Cult 98:157–164
• Wang QC, Gafny R, Sahar N, Sela I, Mawassi M, Tanne E, Perl A (2002) Cryopreservation of grapevine (Vitis vinifera L.) embryogenic cell suspensions and subsequent plant regeneration by encapsulation-dehydration. Plant Sci 162:551–558
• Wang QC, Mawassi M, Sahar N, Li P, Violeta C-T, Gafny R, Sela I, Tanne E, Perl A (2004) Cryopreservation of grapevine (Vitis spp.) embryogenic cell suspensions by encapsulation–vitrification. Plant Cell Tissue Organ Cult 77:267–275
• Wang Q, Laamanen J, Uosukainen M, Valkonen JPT (2005) Cryopreservation of in vitro-grown shoot tips of raspberry (Rubus idaeus L.) by encapsulation–vitrification and encapsulation– dehydration. Plant Cell Rep 24:280–288. doi:10.1007/s00299-005-0936-x
• Xue SH, Luo XJ, Wu ZH, Zhang HL, Wang XY (2008) Cold storage and cryopreservation of hairy root cultures of medicinal plant Eruca sativa Mill. Astragalus membranaceus and Gentiana macrophylla Pall. Plant Cell Tissue Organ Cult 92:251–260
• Yang L, Qin LH, Annie Bligh SW, Bashall A, Zhang CF, Zhang M, Wanga ZT, Xub LS (2006) A new phenanthrene with a spirolactone from Dendrobium chrysanthum and its anti-inflammatory activities. Bioorg Med Chem 14:3496–3501
• Yin M, Hong S (2009) Cryopreservation of Dendrobium candidum Wall. Ex Lindl. protocorm like bodies by encapsulation–vitrification. Plant Cell Tissue Organ Cult 98:179–185
• Yin M, Hong S (2010) A simple cryopreservation protocol of Dioscorea bulbifera L. embryogenic calli by encapsulation–vitrification. Plant Cell Tissue Org Cult 101:349–358

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