Modification of flowers and leaves in Cockscomb (Celosia cristata) ectopically expressing Arabidopsis ASYMMERTIC LEAVES2-LIKE38 (ASL38/LBD41) gene

• Autorzy: Meng L.-S. , Li F. , Sun X.-D. , Liu H. L. , Feng Z. H. , Zhu J.
• Języki publikacji: EN

Abstrakty

EN

ASYMMERTIC LEAVES2-LIKE38 (ASL38/ LBD41), isolated from Arabidopsis, is a member of the LATERAL ORGAN BOUNDARY DOMAIN gene family. We reported that ASL38 might be involved in the dorsoventral determination in extremely folded or crinkly leaves of 35S:ASL38.cockscomb plants; suggesting ASL38 is a transcript factor, and regulates a number of genes that are involved in the morphogenesis and development of plants. To verify this speculation, in this work, we constructed the binary vector pBI121–pMD-18T, which contained the GFP and coding sequences of ASL38, and introduced them into cockscomb via Agrobacterium tumefaciens. We found that ASL38-GFP fusion protein was localized in discrete subnuclear bodies, indicating ASL38 might be a nuclear protein and function as a transcription factor. In modification of flowering plants, many potentially useful genes that are involved in the pathways associated with flower and plant morphology have been cloned. Transcription factors regulating plant development and biosynthetic or regulatory genes involved in plant hormones are common candidates. If 35S:ASL38 cockscomb plants are altered in morphology, these morphological modifications could pave the foundation for the selection of novel flower varieties. As it was speculated by us, in this work, we showed that these 35S:ASL38 cockscomb flowered earlier and their flowers were turned into multiple column patterns, when compared with wild-type cockscomb. Moreover, leaves of some 35S:ASL38 plants revealed lobed and dissected patterns, and extremely, two leaf blades were developed on the same petiole; which was never found in wild-type cockscomb. Together, these morphological modifications of cockscomb indicate that we have successfully attained some novel lines of cockscombs. These lines can have potential practical applications.

Słowa kluczowe

EN

modification; flower; leaf; cockscomb; Celosia cristata; ornamental plant; malformation; Arabidopsis; gene family; gene expression; ASL38 gene; LBD41 gene

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2010
• Tom: 32
• Numer: 2
• Opis fizyczny: p.315-324,fig.,ref.

Twórcy

autor

Meng L.-S.

• School of Life Science and Technology, Tongji University, 200092 Shanghai, China

autor

Li F.

• School of Life Science and Technology, Tongji University, 200092 Shanghai, China

autor

Sun X.-D.

• School of Life Science and Technology, Tongji University, 200092 Shanghai, China

autor

Liu H. L.

• School of Life Science and Technology, Tongji University, 200092 Shanghai, China

autor

Feng Z. H.

• School of Life Science and Technology, Tongji University, 200092 Shanghai, China

autor

Zhu J.

• School of Life Science and Technology, Tongji University, 200092 Shanghai, China

Bibliografia

• Baum SF, Rost TL (1996) Root apical organization in Arabidopsis thaliana: root cap and protoderm. Protoplasma 192:178–188
• Borghi L, Bureau M, Simon R (2007) Arabidopsis JAGGED LATERAL ORGANS is expressed in boundaries and coordinates KNOX and PIN activity. Plant Cell 19:1795–1808
• Chalfun-Junior A, Franken J, Mes JJ, Marsch-Martinez N, Pereira A, Angenent GC (2005) ASYMMETRIC LEAVES2- LIKE1 gene, a member of the AS2/LOB family, controls proximaldistal patterning in Arabidopsis petals. Plant Mol Biol 57:559–575
• Endo T, Shimada T, Fujii H, Kobayashi Y, Araki T, Omura M (2005) Ectopic expression of an FT homolog from Citrus confers an early flowering phenotype on trifoliate orange (Poncirus trifoliata L. Raf.)
• Eshed Y, Baum SF, Perea JV, Bowman JL (2001) Establishment of polarity in lateral organs of plants. Curr Biol 11:1251–1260
• Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL (2004) Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities. Development 131:2997–3006
• Fiuk A, Rybczynski JJ (2008) Morphogenic capability of Gentiana kurroo Royle seedling and leaf explants. Acta Physiol Plant 30:157–166
• Galun E, Breiman A (1997) Transgenic plants. Imperial College Press, London
• Guo M, Thomas J, Collins G, Timmermansa MCP (2008) Direct repression of KNOX loci by the ASYMMETRIC LEAVES1 complex of Arabidopsis. Plant Cell 20:48–58
• Ha CM, Jun JH, Nam HG, Fletcher JC (2007) BLADE-ON-PETIOLE1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes. Plant Cell 19:1809–1825
• Hansen G, Wright MS (1999) Recent advances in the transformation of plants. Trends Plant Sci 4:226–231
• Hu X, Wu QF, Xie YH, Ru H, Xie F, Wang XY et al (2005) Ectopic expression of the PttKN1 gene induces alterations in the morphology of the leaves and flowers in Petunia hybrida Vilm. J Intergrative Plant Biol 47:1153–1158
• Husbands A, Bell EM, Shuai B, Smith HMS, Springer PS (2007) LATERAL ORGAN BOUNDARIES defines a new family of DNA-binding transcription factors and can interact with specific bHLH proteins. Nucleic Acids Res 35:6663–6671
• Inukai Y, Sakamoto T, Ueguchi-Tanaka M, Shibata Y, Gomi K, Umemura I, Hasegawa Y, Ashikari M, Kitano H, Matsuoka M (2005) Crown rootless1, which is essential for crown root formation in rice, is a target of an AUXIN RESPONSE FACTOR in auxin signaling. Plant Cell 17:1387–1396
• Iwakawa H, Ueno Y, Semiarti E, Onouchi H, Kojima S, Tsukaya H, Hasebe M, Soma T, Ikezaki M, Machida C, Machida Y (2002) The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana, required for formation of a symmetric flat lamina, encodes a member of a novel family of proteins characterized by cysteine repeats and a leucine zipper. Plant Cell Physiol 43:467–478
• Kardailsky I, Shukla VK, Ahn JH, Dagenais N, Christensen SK, Nguyen JT, Chory J, Harrison MJ, Weigel D (1999) Activation tagging of the floral inducer FT. Science 286:1962–1965
• Kerstetter RA, Laudencia-Chingcuanco D, Smith LG, Hake S (1997) Lossof- function mutations in the maize homeobox gene, knotted1, are defective in shoot meristem maintenance. Development 124:3045–3054
• Kobayashi Y, Kaya H, Goto K, Iwabuchi M, Araki T (1999) A pair of related genes with antagonistic roles in mediating flowering signals. Science 286:1960–1962
• Kojima S, Takahashi Y, Kobayashi Y, Monna L, Sasaki T, Araki T, Yano M (2002) Hd3a, a rice ortholog of the Arabidopsis FT gene, promotes transition to flowering downstream of Hd1 under short-day conditions. Plant Cell Physiol 43:1096–1105
• Li H, Xu L, Wang H, Yuan Z, Cao X, Yang Z, Zhang D, Xu Y, Huang H (2005) The putative RNA-dependent RNA polymerase RDR6 acts synergistically with ASYMMETRC LEAVES1 and 2 to repress BREVIPEDICELLUS and microRNA165/166 in Arabidopsis leaf development. Plant Cell 17:2157–2171
• Lin WC, Shuai B, Springer PS (2003) The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial–abaxial patterning. Plant Cell 15:2241–2252
• Martin-Trillo M, Martinez-Zapater JM (2002) Growing up fast: manipulating the generation time of trees. Curr Opin Biotechnol 13:151–155
• Meng LS, Ding WQ, Hu X, Wang CY (2009a) Transformation of PttKN1 gene to cockscomb. Acta Physiol Plant 31:683–691
• Meng LS, Song JP, Sun SB, Wang CY (2009b) The ectopic expression of PttKN1 gene causes pleiotropic alternation of morphology in transgenic carnation (Dianthus caryophyllus L.). Acta Physiol Plant 31:1155–1164
• Meng LS, Liu HL, Cui XH, Sun XD, Zhu J (2009c) ASYMMETRIC LEAVES2-LIKE38 Gene, a member of AS2/LOB Family of Arabidopsis, causes leaf dorsoventral alternation in transgenic cockscomb plants. Acta Physiol Plant 31:1301–1306
• Naito T, Yamashino T, Kiba T, Koizumi N, Kojima M, Sakakibara H, Mizuno T (2007) A link between cytokinin and ASL9(ASYMMETRIC LEAVES 2 LIKE 9) that belongs to the AS2/LOB (LATERAL ORGAN BOUNDARIES) family genes in Arabidopsis thaliana. Biosci Biotechnol Biochem 71:1269–1278
• Pekker I, Alvarez JP, Eshed Y (2005) Auxin response factors mediate Arabidopsis organ asymmetry via modulation of KANADI activity. Plant Cell 17:2899–2910
• Pena L, Martin-Trillo M, Juarez J, Pina JA, Navarro L, Martinez-Zapater JM (2001) Constitutive expression of Arabidopsis LEAFY or APETALA1 genes in citrus reduces their generation time. Nat Biotechnol 19:263–267
• Rottman WH, Meilan R, Sheppard LA, Brunner AM, Skinner JS, Ma C, Cheng S, Jouanin L, PilateGand Strauss SH (2000) Diverse effects of overexpression of LEAFY and PTLF, a poplar (Populus) homolog of LEAFY/FLORICAULA, in transgenic poplar and Arabidopsis. Plant J 22:235–245
• Schneeberger R, Tsiantis M, Freeling M, Langdale JA (1998) The ROUGH SHEATH2 gene negatively regulates homeobox gene expression during maize leaf development. Development 125:2857–2865
• Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y (2001) The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves. Development 128:1771–1783
• Shuai B, Reynaga-Peña CG, Springer PS (2002) The LATERAL ORGAN BOUNDARIES gene defines a novel, plant-specific gene family. Plant Physiol 129:747–761
• Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL (1999) Members of the YABBY gene family specify abaxial cell fate in Arabidopsis. Development 126:4117–4128
• Sinha NR, Williams RE, Hake S (1993) Overexpression of the maize homeobox gene KNOTTED-1 causes a switch from determinate to indeterminate cell fates. Genes Dev 7:787–795
• Tanaka Y, Katsumoto Y, Brugliera F, Mason J (2005) Genetic engineering in floriculture. Plant Cell Tiss Organ Cult 80:1–24
• Theodoris G, Inada N, Freeling M(2003)Conservation and molecular dissection of ROUGH SHEATH2 and ASYMMETRIC LEAVES1 function in leaf development. PNAS 100: 6837–6842
• Timmermans MCP, Hudson A, Becraft PW, Nelson T (1999) ROUGH SHEATH2: a Myb protein that represses knox homeobox genes in maize lateral organ primordia. Science 284:151–153
• Tsiantis M, Schneeberger R, Golz JF, Freeling M, Langdale JA (1999) The maize rough sheath2 gene and leaf development programs in monocot and dicot plants. Science 284:154–156
• Ueno Y, Ishikawa T, Watanabe K, Terakura S, Iwakaw H, Okada K, Machida C, Machidaa Y (2007) Histone deacetylases and ASYMMETRIC LEAVES2 are involved in the establishment of polarity in leaves of Arabidopsis. Plant Cell 19:445–457
• Waites R, Selvadurai HRN, Oliver IR, Hudson A (1998) The PHANTASTICA gene encodes a MYB transcription factor involved in growth and dorsoventrality of lateral organs in Antirrhinum. Cell 93:779–789
• Weigel D, Nilsson O (1995) A developmental switch sufficient for flower initiation in diverse plants. Nature 377:495–500
• Xu L, Xu Y, Dong A, Sun Y, Pi L, Xu Y, Huang H (2003) Novel as1 and as2 defects in leaf adaxial-abaxial polarity reveal the requirement for ASYMMETRIC LEAVES1 and 2 and ERECTA functions in specifying adaxial identity. Development 130:4097–4107