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The aim of this study was to determine the vascularisation level in malignant tumours of the oral cavity. The studied material comprised 10 samples of neoplastic tissue extracted from dogs with a suspicion of oral cavity carcinoma. The samples were collected from nine male and one female dogs of various breeds, aged between 6 and 16 years. The tumours were located within the areas of mandibular and maxillary gingiva. Tumour specimens were preserved in 10% buffered formalin for 24 h, embedded in paraffin, and then sections stained with haematoxylin and eosin were prepared. Tumour type was diagnosed according to the official WHO classification. In order to render the vascular endothelium visible, immunohistochemical staining was performed with the use of the polyclonal antibody against von Willebrand factor F VIII and DAKO EnVision system. In the quantitative analysis of the blood vessels displaying the FVIII factor expansion, a system of computer-assisted microscopic analysis was utilised. In ten microscopic fields the vessels were counted and a mean value per 1 mm² was calculated. The largest numbers of blood vessels were observed in squamous cell carcinomas. In the remaining tumours, the vessel count fluctuated between 78-97/mm² (mean 87). The conducted research indicated that angiogenesis in malignant tumours of the oral cavity has potential diagnostic application in determining the malignancy levels of neoplasms.
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Opis fizyczny
p.463-466,fig.,ref.
Twórcy
autor
- Department of Veterinary Surgery, Lublin University of Life Sciences, 20-612 Lublin, Poland
autor
- Department of Veterinary Surgery, Lublin University of Life Sciences, 20-612 Lublin, Poland
autor
- Department of Pathological Anatomy, Faculty of Veterinary Medicine, Lublin University of Life Sciences, 20-612 Lublin, Poland
autor
- Department of Clinical Pathomorphology, Lublin Medical University, 20-090 Lublin, Poland
Bibliografia
- 1. Bradley R.L., MacEwan E.G., Loar A.S.: Mandibular resection for removal of oral tumors in 30 dogs and 6 cats. J Am Vet Med Assoc 1984, 184, 460-463.
- 2. Bradley R.L., Sponenberg D.P., Martin R.A.: Oral neoplasia in 15 dogs and 4 cats. Small Anim Vet Med Surg 1986, 1, 33-42.
- 3. Dore M.: Oral malignant melanoma with osteoid formation in a dog. Vet Pathol 1999, 36, 74-76.
- 4. Folkman J.: Clinical applications of research on angiogenesis. N Engl J Med 1995, 333, 1757-1763.
- 5. Gacko M.: Angiogenesis and methods of diagnosis. Diag Lab 1997, 33, 375-394.
- 6. Grundboeck M.: Epidemiology of neoplastic diseases in animals and humans. Medycyna Wet 1993, 49, 99-102.
- 7. Gryczyński M., Pietruszewska W.: Angiogenesis as a prognostic factor in patients with laryngeal cancer. Otolaryngologia, 2002, 1, 13-20.
- 8. Harvey H.J., MacEwan E.G., Braun D., Patnaik A.K., Withrow S.J., Jongeward S.: Prognostic criteria for dogs with oral melanoma. J Am Vet Med Assoc 1981, 178, 580-582.
- 9. Jeleń-Krzeszewska J., Kręcicki T., Jeleń M., Wnukiewicz J.: Angiogenesis in maxillofacial cancers - study. Dent Med Prob 2004, 41, 615-630.
- 10. Manfra Marretta S.: Recognition and treatment of oral tumors. Atlantic Coast Veterinary Conference, Atlantic City, 2001.
- 11. Morris J., Dobson J.: Small animal oncology. Black Science Ltd, London, 2001.
- 12. Sobczyńska-Rak A., Silmanowicz P., Piórkowski J., Polkowska I., Dębiak P.: Selected oral cavity tumors of dogs. Medycyna Wet 2005, 61, 66-69.
- 13. Sobczyńska-Rak A.: Angiogenesis in skin and subcutaneous tissue of dogs with tumours. Medycyna Wet 2009, 65, 110-114.
- 14. Swidzińska E., Naumnik W., Chyczewska E.: Angiogenesis and neoangiogenesis - the role in lung cancer and other tumors. Pneumonol Alergol Pol 2006, 74, 414-420.
- 15. Szala S., Radzikowski C.: Molecular basis of neoplastic angiogenesis. Nowotwory 1997, 47, 1-19.
- 16. Todoroff R.J., Brody R.S.: Oral and pharyngeal neoplasia in the dogs retrospective survey of 361 cases. J Am Vet Med Assoc 1979, 175, 567-571.
- 17. Verhaert L.: Oral proliferative lesions in dogs and cats. World Small Animal Veterinary Association World Congress, Vancouver, 2001, pp. 242-246.
- 18. Withrow S.: Oral tumors and their biology. World Small Animal Veterinary Association World Congress, Vancouver, 2001, pp. 227-231.
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Bibliografia
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