Herbicide tolerance in maize is related to increased levels of glutathione and glutathione-associated enzymes

• Autorzy: Nemat Alla M.M. , Badawi A.-H. M. , Hassan N. M. , El-Bastawisy Z. M. , Badran E. G.
• Języki publikacji: EN

Abstrakty

EN

Treatment of 10 days old maize seedlings with metribuzin and pretilachlor near the recommended fielddose resulted in differential reductions in shoot fresh and dry weights during the following 16 days. Metribuzin showed great and consistent reductions, however, the reduction induced by pretilachlor, mostly nullified by the end of the experiment. Moreover, there were differential accumulations of lipid peroxides, carbonyl groups and H2O2 in maize leaves; metribuzin caused the greatest accumulation. Meanwhile, levels of thiol forms and reduced glutathione (GSH) were much more induced by pretilachlor than metribuzin; the contrary was true regarding oxidized glutathione (GSSG). The ratio of GSH/ GSSG was highest following pretilachlor treatment and least by metribuzin. On the other hand, activities of glutathione-S-transferases (GSTs, EC 2.5.1.18), c-glutamyl- cysteine synthetase (c-GCS, EC 6.3.2.2), glutathione synthetase (GS, EC 6.3.2.3), glutathione peroxidase (GPX, EC 1.15.1.1) and glutathione reductase (GR, EC 1.6.4.2) were more enhanced in maize leaves by pretilachlor than metribuzin. These findings suggest the occurrence of an oxidative stress differentially induced in maize by the herbicides, a state that was most pronounced with metribuzin. Pretilachlor was concluded to be the least phytotoxic to maize, while metribuzin was the most, this differential tolerance seemed to be related to the induction of GSH and GSH-associated enzymes.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2008
• Tom: 30
• Numer: 3
• Opis fizyczny: p.371-379,fig.,ref.

Twórcy

autor

Nemat Alla M.M.

• Botany Department, Faculty of Science at Damietta, Mansoura University, Damietta, Egypt

autor

Badawi A.-H. M.

• Botany Department, Faculty of Science at Damietta, Mansoura University, Damietta, Egypt

autor

Hassan N. M.

• Botany Department, Faculty of Science at Damietta, Mansoura University, Damietta, Egypt

autor

El-Bastawisy Z. M.

• Botany Department, Faculty of Science at Damietta, Mansoura University, Damietta, Egypt

autor

Badran E. G.

• Botany Department, Faculty of Science at Damietta, Mansoura University, Damietta, Egypt

Bibliografia

• Anderson JV, Davis DG (2004) Abiotic stress alters transcript profiles and activity of glutathione S-transferase, glutathione peroxidase, and glutathione reductase in Euphorbia esula. Physiol Plant 120:421–433
• Anderson MP, Gronwald JW (1991) Atrazine resistance in velvetleaf (Abutilon theophrasi) biotype due to enhanced glutathione S-transferase activity. Plant Physiol 96:104–109
• Aravind P, Prasad MNV (2005) Modulation of cadmium-induced oxidative stress in Ceratophyllum demersum by zinc involves ascorbate–glutathione cycle and glutathione metabolism. Plant Physiol Biochem 43:107–116
• Askelof P, Guthenberg C, Jakobson I, Mannervik B (1975) Purification and characterization of 2 glutathione-S-transferase activities from rate liver. Biochem J 147:513–522
• Buege JA, Aust SD (1972) Microsomal lipid peroxidation. Methods Enzymol 52:302–310
• Chun Yan H, TieBao C, Yu W, BaoHong S (2000) Study on the effects of three herbicides on the growth of maize seedlings. Plant Protect 26:17–19
• Cobb AH, Kirkwood RC (2000) Herbicides and their mechanisms of action. Sheffield Academic Press, Sheffield
• Dewir YH, Chakrabarty D, Ali MB, Hahn EJ, Paek KY (2006) Lipid peroxidation and antioxidant enzyme activities of Euphorbia millii hyperhydric shoots. Environ Exp Bot 58:93–99
• Dixon DP, Edwards R, Robinson NJ, Fordham-Skelton AP, Cole DJ (1995) Spectrum of glutathione transferase activities in maize. Paper presented in Brighton crop protection conference 255–260
• Dvorak J, Remesova I (2002) Assessment of metribuzin effects on potatoes using a method of very rapid fluorescence induction. Rostlinna Vyroba 48:107–117
• Edwards R (1996) Characterization of glutathione transferase and glutathione peroxidases in pea (Pisum sativum). Physiol Plant 98:594–604
• Farago S, Kreuz K, Brunold C (1993) Decreased glutathione levels enhance the susceptibility of maize seedlings to metolachlor. Pestic Biochem Physiol 47:199–205
• Gehin A, Guyon C, Nicod L (2006) Glyphosate-induced antioxidant imbalance in HaCaT: the protective effect of vitamins C and E. Environ Toxic Pharm 22:27–34
• Gupta S, Srivastava AK, Banu N (2005) Setaria cervi: kinetic studies of filarial glutathione synthetase by high performance liquid chromatography. Exp Parasitol 111:137–141
• Hassan NM, Nemat Alla MM (2005) Oxidative stress in herbicidetreated broad bean and maize plants. Acta Physiol Plant 27:429–438
• Iannelli AM, Van Breusegem F, Van Montagu M, Inze D, Massacci A (1999) Tolerance to low temperature and paraquat-mediated oxidative stress in two maize genotypes. J Exp Bot 50:523–532
• Jablonkai I, Hatzios K (1993) In vitro conjugation of chloroacetanilide herbicides and atrazine with thiols and contribution of nonenzymatic conjugation to their glutathione-mediated metabolism in corn. J Agric Food Chem 41:1736–1742
• Kearney PC, Kaufman DD (1988) Herbicides: chemistry, degradation and mode of action, vol. 3, Dekker, New York
• Kuzniak E (2002) Transgenic plants: an insight into oxidative stress tolerance mechanisms. Acta Physiol Plant 24:97–113
• Levine RL, Williams JA, Stadtman ER, Shacter E (1991) Carbonyl assays for determination of oxidatively modified proteins. Methods Enzymol 233:346–363
• May M, Vernoux T, Leaver C, Van Montagu M, Inze D (1998) Glutathione homeostasis in plants: implications for environmental sensing and plant development. J Exp Bot 49:649–667
• Mendoza-Cózatl DG, Moreno-Sánchez R (2006) Control of glutathione and phytochelatin synthesis under cadmium stress. Pathway modeling for plants. J Theor Biol 238:919–936
• Misra N, Mohd Saquib A, Gupta AK (2006) Differential response of scavenging of reactive oxygen species in green gram genotype grown under salinity stress. Am J Plant Physiol 1:41–53
• Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410
• Nagalakshmi N, Prasad MNV (2001) Reponses of glutathione cycle enzymes and glutathione metabolism to copper stress in Scenedesmus bijugatus. Plant Sci 160:291–299
• Nayyar H, Gupta D (2006) Differential sensitivity of C3 and C4 plants to water deficit stress: association with oxidative stress and antioxidants. Environ Exp Bot 58:106–113
• Nemat Alla MM, Hassan NM (2006) Changes of antioxidants levels in two maize lines following atrazine treatments. Plant Physiol Biochem 44:202–210
• Nemat Alla MM, Hassan NM (2007) Changes of antioxidants and GSH-associated enzymes in isoproturon-treated maize. Acta Physiol Plant 29:247–258
• Nemat Alla MM, Badawi AM, Hassan NM, El-Bastawisy ZM, Badran EG (2007) Induction of glutathione and glutathioneassociated enzymes in butachlor-tolerant plant species. Am J Plant Physiol 2:195–205
• Nemat Alla MM, Badawi AM, Hassan NM, El-Bastawisy ZM, Badran EG (2008) Effect of metribuzin, butachlor and chlorimuron-ethyl on amino acid and protein formation in wheat and maize seedlings. Pestic Biochem Physiol 90:8–18
• Okuda T, Masuda Y, Yamanaka A, Sagisaka S (1991) Abrupt increase in the level of hydrogen peroxide in leaves of winter wheat is caused by cold treatment. Plant Physiol 97:1265–1267
• Polidoros AN, Scandalios JG (1999) Role of hydrogen peroxide and different classes of antioxidants in the regulation of catalase and glutathione S-transferase gene expression in maize (Zea mays L.). Physiol Plant 106:112–120
• Pyon JY, Piao RZ, Roh SW, Shin SY, Kwak SS (2004) Differential levels of antioxidants in paraquat-resistant and susceptible Erigeron canadensis biotypes in Korea. Weed Biol Manag 4:75–80
• Reinheckel T, Noack H, Lorenz S, Wiswedel I, Augustin W (1998) Comparison of protein oxidation and aldehyde formation during oxidative stress in isolated mitochondria. Free Radical Res 29:297–305
• Sandalio LM, Dalurzo HC, Gomez M, Romero-Puetas MC, del Rio LA (2001) Cadmium-induced changes in the growth and oxidative metabolism of pea plants. J Exp Bot 52:2115–2126
• Scarponi L, Perucci P, Martinetti L (1991) Conjugation of 2-chloroacetanilide herbicides with glutathione: role of molecular structures and of glutathione S-transferase enzymes. J Agric Food Chem 39:2010–2013
• Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue by Ellman’s reagent. Anal Biochem 25:192–208
• Smith IK, Vierheller TL, Thorne CA (1988) Assay of glutathione reductase in crude tissue homogenates using 5,5-dithiobis-(2-nitrobenzoic acid). Anal Biochem 175:408–413
• Snedecor W, Cochran G (1980) Statistical methods, 7th edn. The Iowa State University Press, Ames
• Volohonsky G, Tuby CN, Porat N, Wellman-Rousseau M, Visvikis A, Leroy P, Rashi S, Steinberg P, Stark A (2002) A spectrophotometric assay of c-glutamylcysteine synthetase and glutathione synthetase in crude extracts from tissues and cultured mammalian cells. Chem Biol Interact 140:49–65
• Ye B, Faltin Z, Ben-Hyyim G, Eshdat Y, Gressel J (2000) Correlation of glutathione peroxidase to paraquat/oxidative stress resistance in Conyza determined by direct fluorometric assay. Pestic Biochem Physiol 66:182–194
• Zabalza A, Gaston S, Sandalio LM, del Río LA, Royuela M (2007) Oxidative stress is not related to the mode of action of herbicides that inhibit acetolactate synthase. Environ Exp Bot 59:150–159

Uwagi

Rekord w opracowaniu