Environmental-dependent proline accumulation in plants living on gypsum soils

• Autorzy: Boscaiu M. , Bautista I. , Lidon A. , Llinares J. , Lull C. , Donat P. , Mayoral O. , Vincente O.
• Języki publikacji: EN

Abstrakty

EN

Biosynthesis of proline—or other compatible solutes—is a conserved response of all organisms to different abiotic stress conditions leading to cellular dehydration. However, the biological relevance of this reaction for plant stress tolerance mechanisms remains largely unknown, since there are very few available data on proline levels in stress-tolerant plants under natural conditions. The aim of this work was to establish the relationship between proline levels and different environmental stress factors in plants living on gypsum soils. During the 2-year study (2009–2010), soil parameters and climatic data were monitored, and proline contents were determined, in six successive samplings, in ten taxa present in selected experimental plots, three in a gypsum area and one in a semiarid zone, both located in the province of Valencia, in south-east Spain. Mean proline values varied significantly between species; however, seasonal variations within species were in many cases even wider, with the most extreme differences registered in Helianthemum syriacum (almost 30 μmol g-1 of DW in summer 2009, as compared to ca. 0.5 in spring, in one of the plots of the gypsum zone). Higher proline contents in plants were generally observed under lower soil humidity conditions, especially in the 2009 summer sampling preceded by a severe drought period. Our results clearly show a positive correlation between the degree of environmental stress and the proline level in most of the taxa included in this study, supporting a functional role of proline in stress tolerance mechanisms of plants adapted to gypsum. However, the main trigger of proline biosynthesis in this type of habitat, as in arid or semiarid zones, is water deficit, while the component of ‘salt stress’ due to the presence of gypsum in the soil only plays a secondary role.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 07
• Opis fizyczny: p.2193-2204,fig.,ref.

Twórcy

autor

Boscaiu M.

• Instituto Agroforestal Mediterra´neo (IAM, UPV), Universitat Polite`cnica de Vale`ncia, Camino de Vera s/n, 46022 Valencia, Spain

autor

Bautista I.

• RE-FOREST, Departamento de Ingenierı´a Hidra´ulica y Medio Ambiente, Universitat Polite`cnica de Vale`ncia, Camino de Vera s/n, 46022 Valencia, Spain

autor

Lidon A.

• Escuela Te´cnica Superior de Ingenierı´a Agrono´mica y del Medio Natural, Universitat Polite`cnica de Vale`ncia, Edificio 3I, Planta baja, Camino de Vera s/n, 46022 Valencia, Spain e-mail:
• RE-FOREST, Departamento de Ingenierı´a Hidra´ulica y Medio Ambiente, Universitat Polite`cnica de Vale`ncia, Camino de Vera s/n, 46022 Valencia, Spain

autor

Llinares J.

• Instituto de Investigacio´n para la Gestio´n Integral de Zonas Costeras (IGIC, UPV), Universitat Polite`cnica de Vale`ncia, Calle Paranimf 1, 46730 Gandia, Spain

autor

Lull C.

• RE-FOREST, Departamento de Ingenierı´a Hidra´ulica y Medio Ambiente, Universitat Polite`cnica de Vale`ncia, Camino de Vera s/n, 46022 Valencia, Spain

autor

Donat P.

• Instituto de Investigacio´n para la Gestio´n Integral de Zonas Costeras (IGIC, UPV), Universitat Polite`cnica de Vale`ncia, Calle Paranimf 1, 46730 Gandia, Spain

autor

Mayoral O.

• Instituto de Investigacio´n para la Gestio´n Integral de Zonas Costeras (IGIC, UPV), Universitat Polite`cnica de Vale`ncia, Calle Paranimf 1, 46730 Gandia, Spain

autor

Vincente O.

• Instituto de Biologı´a Molecular y Celular de Plantas (IBMCP, UPV-CSIC), Universitat Polite`cnica de Vale`ncia, Camino de Vera s/n, 46022 Valencia, Spain

Bibliografia

• Alvarado JJ, Ruiz JM, López-Cantarero I, Molero J, Romero L (2000) Nitrogen metabolism in five plant species characteristic of gypsiferous soils. J Plant Physiol 156:612–616
• Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59:206–216
• Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207
• Briens M, Larher F (1982) Osmoregulation in halophytic higher plants: a comparative study of soluble carbohydrates, polyols, betaines and free proline. Plant, Cell Environ 5:287–292
• Burriel F, Hernando V (1947) Nuevo método para determinar el fósforo asimilable en los suelos. Anales de Edafologıa y Fisiologıa Vegetal 9:611–622
• Caballero I, Olano JM, Loidi J, Escudero A (2003) Seed bank structure along a semi-arid gypsum gradient in Central Spain. J Arid Environ 55:287–299
• Escudero A, Carnes LF, Pérez Garcıa F (1997) Seed germination of gypsophytes and gypsovags in semi-arid central Spain. J Arid Environ 36:487–497
• Escudero A, Somolinos RC, Olano JM, Rubio A (1999) Factors controlling the establishment of Helianthemum squamatum, an endemic gypsophite of semi-arid Spain. J Ecol 87:290–302
• FAO (1990) Management of gypsiferous soils. FAO Soils Bull 62 Ferriol M, Pérez I, Merle H, Boira H (2006) Ecological germination requirements of the aggregate species Teucrium pumilum (Labiatae) endemic to Spain. Plant Soil 284:205–216
• Flowers TJ, Colmer TD (2008) Salinity tolerance in halophytes. New Phytol 179:945–963
• Flowers TJ, Troke PF, Yeo AR (1977) The mechanism of salt tolerance in halophytes. Ann Rev Plant Physiol 28:89–121
• Gil R, Lull C, Boscaiu M, Bautista I, Lidón A, Vicente O (2011) Soluble carbohydrates as osmolytes in several halophytes from a Mediterranean salt marsh. Not Bot Horti Agrobo 39(2): 9–17
• Grigore MN, Boscaiu M, Vicente O (2011) Assessment of the relevance of osmolyte biosynthesis for salt tolerance of halophytes under natural conditions. Eur J Plant Sci Biotech 5:12–19
• Hare PD, Cress WA, Van Standen J (1998) Dissecting the roles of osmolyte accumulation during stress. Plant Cell Environ 21:535–553
• Keeney DR, Nelson DW (1982) Nitrogen inorganic forms. In: Page AL et al (eds) Methods of soil analysis, part 2: chemical and microbiological properties. Soil Science Society of America, Madison, pp 643–698
• Knudsen D, Peterson GA, Pratt PF (1982) Lithium, Sodium and Potassium. In: Page AL et al (eds) Methods of soil analysis, part 2: chemical and microbiological properties. Soil Science Society of America, Madison, pp 225–246
• Kuo S (1996) Phosphorus. In: Spark DL (ed) Methods of soil analysis: chemical methods, part 3. Soil Science Society of America, Madison, pp 869–919
• Martens H, Maes T (1989) Multivariate calibration. Wiley, New York, pp 97–108
• Martınez-Duro E, Ferrandis P, Escudero A, Luzuriaga AL, Herranz JM (2010) Secondary old-field succession in an ecosystem with restrictive soils: does time from abandonment matter? Appl Veg Sci 13:234–248
• Meyer SE (1986) The ecology of gypsophile endemism in the eastern Mojave desert. Ecology 67:1303–1313
• Meyer SE, Garcıa-Moya E (1989) Plant community patterns and soil moisture regime in gypsum grasslands of north central Mexico. J Arid Environ 16:147–155
• Meyer SE, Garcıa-Moya E, Lagunes-Espinoza LC (1992) Topographic and soil surface effects on gypsophile plant community patterns in central Mexico. J Veg Sci 3:429–438
• Moruno F, Soriano P, Vicente O, Boscaiu M, Estrelles E (2011) Opportunistic germination behaviour of Gypsophila (Caryophyllaceae) in two priority habitats from semi-arid Mediterranean steppes. Not Bot Horti Agrobo 39(1):18–23
• Mota JF, Sánchez Gomez P, Merlo Calvente ME, Catalán Rodrıguez P, Laguna Lumbreras E, de la Cruz Rot M, Navarro Reyes FB, Marchal Gallardo F, Bartolomé Esteban C, Martınez Labarga JM, Sainz Ollero H, Valle Tendero F, Serra Laliga L, Martınez Hernández F, Garrido Becerra JA, Pérez Garcıa FJ (2009) Aproximación a la checklist de los gipsófitos ibéricos. Anales de Biologıa 31:71–80
• Murakeözy E P, Nagy Z, Duhazé C, Bouchereau A, Tuba Z (2003) Seasonal changes in the levels of compatible osmolytes in three halophytic species of inland saline vegetation in Hungary. J Plant Physiol 160:395–401
• Nelson DW, Sommers LE (1982) Total carbon, organic carbon, and organic matter. In: Page AL et al (eds) Methods of soil analysis, part 2: chemical and microbiological properties. Soil Science Society of America, Madison, pp 539–577
• Palacio S, Escudero A, Montserrat-Martı G, Maestro M, Milla R, Albert M (2007) Plants living on gypsum: beyond the specialist model. Ann Bot 99:333–343
• Parsons RF (1977) Gypsophily in plants—a review. Am Midl Nat 96:1–20
• Pueyo Y, Alados CL, Maestro M, Komac B (2007) Gypsophile vegetation patterns under a range of soil properties induced by topographical position. Plant Ecol 189:301–311
• Rivas-Martınez S, Rivas-Sáenz S (2009) Worldwide Bioclimatic Classification System. Phytosociological Research Center, Complutense University of Madrid, Spain. http://www.globalbioclimatics.org/. Accessed 15 Nov 2012
• Romão RL, Escudero A (2005) Gypsum physical soil crusts and the existence of gypsophytes in semi-arid central Spain. Plant Ecol 181:127–137
• Rubio A, Escudero A (2000) Small-scale spatial soil-plant relationship in semi-arid gypsum environment. Plant Soil 220:139–150
• Ruız JM, López-Cantarero I, Rivero RM, Romero L (2003) Sulphur phytoaccumulation in plant species characteristic of gypsiferous soils. Int J Phytorem 5:203–210
• Szabados L, Savouré A (2010) Proline: a multifunctional amino acid. Trends Plant Sci 15:89–97
• Szabados L, Kovács H, Zilberstein A, Bouchereau A (2011) Plants in extreme environments: importance of protective compounds in stress tolerance. Adv Bot Res 57:105–150
• Tecator Application Note (1984) AN 5226: Determination of ammonium in 2 M KCl soil extracts by FIAstar 5000. AN 5201: Determination of the sum of nitrate and nitrite in water by FIAstar 5000. (Adapted for 2 M KCl soil extracts)
• Tipirdamaz R, Gagneul D, Duhazé C, Aı¨nouche A, Monnier C, Özkum D, Larher F (2006) Clustering of halophytes from an
• inland salt marsh in Turkey according to their ability to accumulate sodium and nitrogenous osmolytes. Environ Exp Bot 57:139–153
• Verheye WH, Boyadgiev TG (1997) Evaluating the land use potential of gypsiferous soils from field pedogenic characteristics. Soil Use Manage 13:97–103
• Vicente O, Boscaiu M, Naranjo MA, Estrelles E, Bellés JM, Soriano P (2004) Responses to salt stress in the halophyte Plantago crassifolia (Plantaginaceae). J Arid Environ 58:463–481
• Yancey PH (2005) Organic osmolytes as compatible, metabolic and counteracting cytoprotectants in high osmolarity and other stresses. J Exp Biol 208:2819–2830

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