Comparative study of different methods for plasma membrane integrity assessment of frozen-thawed boar spermatoza

• Autorzy: Fraser L. , Zasiadczyk L. , Lecewicz M. , Kordan W.
• Języki publikacji: EN

Abstrakty

EN

In this study, different assays were used to assess the structural and functional integrity of the sperm plasma membrane following freezing-thawing of the whole ejaculates (WEs) and sperm-rich fractions (SRFs) of boar semen. Besides sperm viability assessments (motility and mitochondrial membrane potential using a mitochondrial specific dye, JC-1 with propidium iodide, PI), sperm plasma membrane integrity (PMI) assessments were determined simultaneously using different membrane-based tests (SYBR- 14/PI and carboxyfluorescein diacetate (CFDA) with PI (CFDA/PI), and the hypo-osmotic swelling (HOS) test). ANOVA results showed that boar variability had a significant effect on the analysed parameters of post-thaw sperm characteristics. Spermatozoa harvested from the WEs exhibited a marked decline in post-thaw viability, manifested in reduced motility and mitochondrial membrane potential, than those originated from the SRFs after freezing-thawing. Cryopreservation compromised sperm PMI, as indicated in a significant decline in SYBR-stained, CFDA-stained or HOS-positive sperm cells, irrespective of the ejaculate collection procedure. It was observed that the membrane-based tests were strongly inter-correlated. Furthermore, agreement between the measurements of the membrane-based tests was confirmed by the Bland-Altman scatter plots of differences, suggesting that these tests could detect the same sperm cohorts, which were susceptible to cryo-induced membrane damage. The findings of this study indicate that dual fluorescent staining with SYBR-14/PI and CFDA/PI assays, in combination with the HOS test, provide more precise description of the sperm populations in frozen-thawed boar semen.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2011
• Tom: 55
• Numer: 2
• Opis fizyczny: p.231-236,fig.,ref.

Twórcy

autor

Fraser L.

• Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, Warmia and Mazury University in Olsztyn, Poland

autor

Zasiadczyk L.

autor

Lecewicz M.

autor

Kordan W.

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