Isolation and characterization of a C-repeat binding factor (CBF)-like gene in cassava (Manihot esculenta Crantz)

• Autorzy: Li R. , Ji Y. , Fan J. , Yang C. , Yao Y. , Zhou Y. , Duan R. , Liu J. , Fu S. , Hu X. , Guo J.
• Języki publikacji: EN

Abstrakty

EN

Cassava (Manihot esculenta Crantz) is a tropical and subtropical plant and susceptible to chilling injury. In this research, a C-repeat binding factor (CBF)-like gene (GenBank accession number JQ339740) has been isolated from cassava, and named as MeCBF1. The full-length DNA of MeCBF1 is 1,037 base pair (bp), without intron. The 5' untranslated region is 102 bp, the 3' untranslated region is 239 bp, and the open reading frame is 696 bp encoding 231 amino acids. The deduced amino acid sequence of MeCBF1 contains two CBF conserved motifs of PKK(P/R)AGRxKFxETRHP and DSxWR. The MeCBF1 shows 83 % homology to the CRT/DRE binding factor 1 from Hevea brasiliensis (Accession no. AAY43213.1). However, in cassava, the MeCBF1 target genes showed low similarity to the CBF/DREB regulated genes in Arabidopsis thaliana. Quantitative real-time PCR showed that the MeCBF1 was highly expressed in stems and leaves, and lowly expressed in roots. In addition, the expression of the MeCBF1 quickly responded to low temperature stress (4°C). These results suggest that, the MeCBF1 is functional in cassava. Further studies on the MeCBF1 might be helpful to reveal molecular mechanism of cassava’s high sensitivity to low temperature.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 11
• Opis fizyczny: p.3089-3093,fig.,ref.

Twórcy

autor

Li R.

• Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Ministry of Agriculture, Haikou, 570711, China

autor

Ji Y.

• Hainan Agricultural Science and Technology 110 Co. LTD, Haikou, 570102, China

autor

Fan J.

• Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Ministry of Agriculture, Haikou, 570711, China
• College of Agriculture, Hainan University, Haikou, 570228, China

autor

Yang C.

• Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Ministry of Agriculture, Haikou, 570711, China
• College of Agriculture, Hainan University, Haikou, 570228, China

autor

Yao Y.

• Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Ministry of Agriculture, Haikou, 570711, China
• College of Agriculture, Hainan University, Haikou, 570228, China

autor

Zhou Y.

• Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Ministry of Agriculture, Haikou, 570711, China

autor

Duan R.

• Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Ministry of Agriculture, Haikou, 570711, China

autor

Liu J.

• Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Ministry of Agriculture, Haikou, 570711, China

autor

Fu S.

• Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Ministry of Agriculture, Haikou, 570711, China

autor

Hu X.

• College of Agriculture, Hainan University, Haikou, 570228, China

autor

Guo J.

• Key Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Ministry of Agriculture, Haikou, 570711, China

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Identyfikatory

DOI

10.1007/s11738-014-1664-z