Heterogeneity of the local tissue microenvironment influences differentiation of NG2 cells

• Autorzy: Sypecka J. , Sarnowska A. , Szablowska-Gadomska I. , Lukomska D. , Domanska-Janik K.
• Języki publikacji: EN

Abstrakty

EN

The NG2 cells are the oligodendrocyte precursors that terminally differentiated are capable for myelination of central nervous system (CNS). They exhibit many features of neural stem cells and constitute the abundant population of dividing progenitors in the young and adult brain. A question arises if their commitment could be modulated by local tissue-specific or neuropathological signals. The aim of our study therefore was to evaluate the effect of distinct microenvironments (provided by either the spinal cord or the hippocampal slices) on the differentiation of neonatal NG2 cells. Subsequently, hippocampal slice culture subjected to an ischemic injury (the glucose-oxygen deprivation, OGD) was used in order to evaluate the cell development in microenvironment conditioned by traumatized tissue. Methods. Both the hippocampal and spinal cord slice cultures were established from the same 7-day old rats. The model of an indirect contact (i.e. exclusively by the culture media) in co-culture system was chosen to eliminate the influence of cell-cell contact. The NG2 cells were obtained from 10-day old mixed primary culture of neonatal rat hemispheres. After 7 days in co-culture, the cells were either stained with neural markers or collected for the RNA isolation and real-time PCR. Results. The medium conditioned by hippocampal slices effectively promoted neurogenesis: ~30 % of NG2 cells differentiated into TUJ 1-positive neurons. The remaining fraction mostly formed premyelinating and mature oligodendrocytes. The exposition of hippocampal slices to the OGD injury abolished the effect of pro-neuronal induction in co-cultures. In media conditioned by spinal cord slices, neurogenesis was less pronounced (20% neurons) and the oligodendrocyte differentiation was significantly slowed-down. Conclusions. The NG2 cells were shown to have intrinsic potency for neurogenesis. Heterogeneity of local microenvironment might modify the fate of endogenous or transplanted NG2 cells what should be taken into consideration in potential neurorepair strategies. Supported by grant 0345/B/P01/2010/38.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: 1

Twórcy

autor

Sypecka J.

• Medical Research Centre, NeuroRepair Department, Polish Academy of Sciences, Warsaw, Poland

autor

Sarnowska A.

• Medical Research Centre, NeuroRepair Department, Polish Academy of Sciences, Warsaw, Poland

autor

Szablowska-Gadomska I.

• Medical Research Centre, NeuroRepair Department, Polish Academy of Sciences, Warsaw, Poland

autor

Lukomska D.

• Medical Research Centre, NeuroRepair Department, Polish Academy of Sciences, Warsaw, Poland

autor

Domanska-Janik K.

• Medical Research Centre, NeuroRepair Department, Polish Academy of Sciences, Warsaw, Poland