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2009 | 59 | 1 |
Tytuł artykułu

Bacteria of Bacillus cereus group in cereals at retail

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EN
Abstrakty
EN
The subject of analysis were three main types of cereals available at retail on the local market. The objective was to assess the extent of cereals contamination by B. cereus group and to indicate the species dominant in cereals contamination. Bacteria of B. cereus group were present in 89.6% of the retail packs tested. The isolation frequency of B. cereus from pearl barley, buckwheat and wheat cereals was 85%, 85.7% and 100%, respectively, with contamination level, in majority of the analysed samples (60.4%), exceeding 102 MPN/g. The strains prevailing in cereals contamination did not hydrolyse starch and were β hemolytic. Out of the 380 isolated strains, those not hydrolysing starch accounted for 56%, with only 16 strains (4.2%) not able both to hydrolyse starch and non-hemolytic. Amongst the strains growing at 4° to 30° C the ability to hydrolyse starch and to perform β hemolysis – the features attributed to diarrheal strains – was expressed by 36.9% strains. Twenty strains (5.3% of the total number) – non-hemolytic, unable to hydrolyse starch and not growing at 4° C – were considered as potentially emetic ones. Mesophilic, lecithinase-positive strains, β hemolysis-positive, unable to hydrolyse starch predominant in the tested cereals (208/380) were identi­fied with the API 50 CHB tests (Bio Merieux) as B. cereus type 1. B. cereus type 1 was the prevailing contaminant in barley and wheat cereals. In buckwheat, the most commonly isolated species was B. mycoides.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
59
Numer
1
Opis fizyczny
p.53-59,ref.
Twórcy
  • Department of Food Microbiology, Agricultural University, P.Pawla VI/4, 71-459 Szczecin, Poland
autor
autor
Bibliografia
  • 1. Altayar M., Sutherland A.D., Bacillus cereus is common in the environment but emetic toxin producing isolates are rare. J. Appl. Microbiol., 2006, 100, 7–14.
  • 2. Anonymous 1, State of households in 2006 based on households budgets results. Proceedings of Press Conf., June 26th 2007, ed. GUS-Dept. Statystyki Społeczne, 2007, pp. 11–12, [http://www.stat.gov.pl/cps/rde/ xbcv/gus/PUBL sytuacja gosp dom 2006 pub0706.pdf], (in Polish).
  • 3. Aziz N.H., Souzan R M., Shahin A.A., Effect of γ-irradiation on the occurrence of pathogenic microorganisms and nutritive value of four principal cereal grains. Appl. Radiat. Isot., 2006, 64, 1555–1562.
  • 4. Carlin F., Fricker M., Pielaat A., Heisterkamp S., Shaheen R., Salkinoja Salonen M., Svensson B., Nguyen-The C., Ehling‑Schulz M., Emetic toxin-producing strains of Bacillus cereus show distinct characteristics within the Bacillus cereus group. Int. J. Food Microbiol., 2006, 109, 132–138.
  • 5. Drobniewski F.A., Bacillus cereus and related species. Clin. Microbiol. Rev., 1993, 6, 324–338.
  • 6. EC Regulation No. 2073/2005 of the November 5th, 2005 on microbiological criteria for foodstuffs (in Polish).
  • 7. Goepfert J.M., Spira W.M., Kim H.U., Bacillus cereus: food poisoning organism. A review. J. Milk Food Technol., 1972, 35, 213–227.
  • 8. Haque A., Russell N., Phenotypic and genotypic characterisation of Bacillus cereus isolates from Bangladeshi rice. Int. J. Food Microbiol., 2005, 98, 23–34.
  • 9. Hendriksen N.B., Hansen B.M., Johansen J.E., Occurrence and pathogenic potential of Bacillus cereus group bacteria in a sandy loam. Antonie van Leeuwenhoek, 2005, 89, 239–249.
  • 10. Holbrook R., Anderson J.M., An improved selective and diagnostic medium for the isolation and enumeration of Bacillus cereus in foods. Can. J. Microbiol., 1980, 26, 753–759.
  • 11. IMNRC (Institute of Medicine National Research Council): Scientific Criteria to Ensure Safe Food. 2003. The National Academic Press, Washington D.C. [www.nap.edu], Appendix E, pp. 317–358.
  • 12. Johnson K.M., Bacillus cereus in foodborne illness – an update. J. Food Prot., 1984, 47, 145–153.
  • 13. Lechner S., Mayr R., Francis K.P., Pruss B.M., Kaplan T., Wiessner-Gunkel E., Stewart G.S.A.B., Scherer S., Bacillus weihenstephanensis sp. nov. is a new psychrotolerant species of the Bacillus cereus group. Int. J. Syst. Bacteriol., 1998, 48, 1373–1382.
  • 14. Nakamura L.K., Bacillus pseudomycoides sp.nov. Int. J. Syst. Bacteriol., 1998, 48, 1031–1035.
  • 15. Norris J.R., Berkeley R.C. W., Logan N.A., O’Donnell A.G., The genera Bacillus and Sporolactobacillus. 1981, in: The Prokaryotes – A Handbook on Habitats, Isolation and Identification of Bacteria, vol. II, (eds. M.P. Starr et al.). Springer – Verlag, Berlin, pp. 1711–1742.
  • 16. Pirhonen T.I., Andersson M.A., Jääskeläinen E.L., Salkinoja-Salonen M.S., Honkanen-Buzalski T., Johansson T.M.-L., Biochemical and toxic diversity of Bacillus cereus in pasta and meat dish associated with a food poisoning case. Food Microbiol., 2005, 22, 87–91.
  • 17. Polish Standard PN-A-74205:1997. Cereals, gruels and corn meal (in Polish).
  • 18. Polish Standard PN-EN ISO 21871:2007. Microbiology of food and feedstuffs. Horizontal method of determination of low numbers of presumptive Bacillus cereus. Detection and determination of most probable number (in Polish).
  • 19. Polish Standard PN-V-74003:2004. Barley cereal special (in Polish).
  • 20. Polish Standard PN-V-74004:2004. Buckwheat special (in Polish).
  • 21. Sarrias J., Valero M., Salmeron M., Enumeration, isolation and characterization of Bacillus cereus strains from Spanish raw rice. Food Microbiol., 2002, 19, 589–595.
  • 22. Świderski F., Commodity Science of Processed Food. 1999, 1st ed., SGGW, Warszawa, pp. 317–324 (in Polish).
  • 23. Thorsen L., Hansen B.M., Nielsen K.F., Hendiksen N.B., Phipps R.K., Budde B.B., Characterization of emetic Bacillus weihenstephanensis a new cereulide-producing bacterium. Appl. Environ. Microbiol., 2006, 72, 5118–5121.
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  • 25. Zaremba M.L., Borowski J., Medicine Microbiology. 2001, 2nd ed. PZWL, Warszawa, p. 302 (in Polish).
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