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Abstrakty
Polyphenol compounds were analysed and storage stability determined of cold-pressed cakes from one non-genetically modified (non-GM) flax variety Linola and two GM lines: W92 line with overexpressed flavonoid biosynthesis pathway and GT line with overexpressed glycosyltransferase. The 20 g samples of each cake were stored in oxygen-free and dark conditions for 3 (T3) and 7 (T7) months at 4 and 21 °C. Initial (T0), and T3 and T7 samples were analysed for phenolic acids, phenolic acid glucosides and secoisolariciresinol diglucoside (SDG) content, and peroxide, free fatty acids and 2-thiobarbituric acid (TBA) values; total viable counts (TVC) and counts of yeast and moulds were also determined. At T0, T3 and T7, contents of all phenolic compounds were higher in both GM than non-GM cakes and in all cakes most of them were slightly decreased at T3 and more at T7. At T0 peroxide, free fatty acid and TBA values were lower in GM than in non-GM cake (particularly of TBA in W92 with the highest initial SDG content) indicating their better initial fat quality. Small changes of the indices reflecting fat deterioration during storage seemed, however, to be not dependent on polyphenol content. The TVC at T0, T3 and T7/4 °C were the smallest in W92 cake and did not change in a regular way in non-GM and GT cakes. Yeast counts were low in all cakes over the whole storage period and mould counts were the smallest in W92 cake. It may be concluded that protective properties of flax polyphenols, especially of SDG present in W92, were more apparent prior to than during cake storage.
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p.70-73,ref.
Twórcy
autor
- Department of Animal Nutrition and Biotechnology, Faculty of Animal Sciences, Warsaw University of Life Sciences - SGGW, Ciszewskiego 8, 02-786 Warsaw, Poland
autor
- Department of Animal Nutrition and Biotechnology, Faculty of Animal Sciences, Warsaw University of Life Sciences - SGGW, Ciszewskiego 8, 02-786 Warsaw, Poland
autor
- Department of Genetics and Animal Breeding, Faculty of Animal Sciences, Warsaw University of Life Sciences - SGGW, Ciszewskiego 8, 02-786 Warsaw, Poland
autor
- Department of Genetic Biochemistry, Faculty of Biotechnology, University of Wrocław, Przybyszewskiego 63/77, 51-148 Wrocław, Poland
autor
- Department of Genetic Biochemistry, Faculty of Biotechnology, University of Wrocław, Przybyszewskiego 63/77, 51-148 Wrocław, Poland
Bibliografia
- AOAC International, 2012. Official Methods of Analysis of AOAC International. 19th Edition. Gaithersburg, MD (USA)
- AOCS, 2004. Official Methods and Recommended Practices of the American Oil Chemists’ Society. 5th Editon. Champaign, IL (USA)
- Barriuso B., Astiasarán I., Ansorena D., 2013. A review of analytical methods measuring lipid oxidation status in foods: a challenging task. Eur. Food Res. Technol. 236, 1–15, https://doi.org/10.1007/s00217-012-1866-9
- Bravi E., Perretti G., Marconi O., Patrizi E., Fantozzi P., 2011. Secoisolariciresinol diglucoside determination in flaxseed (Linum usitatissimum L.) oil and application to a shelf life study. Food Chem. 126, 1553–1558, https://doi.org/10.1016/j.foodchem.2010.11.169
- ISO 21527-2:2008. Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of yeasts and moulds – Part 2: Colony count technique in products with water activity less than or equal to 0.95. International Standards Organization. Geneva (Switzerland)
- ISO 4833:2003. Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of microorganisms – Colony-count technique at 30 °C. International Standards Organization. Geneva (Switzerland)
- Lorenc-Kukuła K., Zuk M., Kulma A., Czemplik M., Kostyn K., Skala J., Starzycki M., Szopa J., 2009. Engineering flax with the GT family 1 Solanum sogarandinum glycosyltransferase SsGT1 confers increased resistance to Fusarium infection. J. Agric. Food Chem. 57, 6698–6705, https://doi.org/10.1021/jf900833k
- Manthey F.A., Sinha S., Wolf-Hall C.E., Hall C.A. III, 2008. Effect of flaxseed flour and packaging on shelf life of refrigerated pasta. J. Food Process. Preserv. 32, 75–87, https://doi.org/10.1111/j.1745-4549.2007.00166.x
- Matusiewicz M., Kosieradzka I., Sobczak-Filipiak M., Zuk M., Szopa J., 2015. Transgenic flax overexpressing polyphenols as a potential anti-inflammatory dietary agent. J. Funct. Foods 14, 299–307, https://doi.org/10.1016/j.jff.2015.02.004
- Mercier S., Villeneuve S., Moresoli C., Mondor M., Marcos B., Power K.A., 2014. Flaxseed-enriched cereal-based products: a review of the impact of processing conditions. Comp. Rev. Food Sci. Food Saf. 13, 400-412, https://doi.org/10.1111/1541-4337.12075
- Pokorny J., Dieffenbacher A., 1989. Determination of 2-thiobarbituric acid value: direct method – results of a collaborative study and the standarised method. Pure Appl. Chem. 61, 1165–1170, https://doi.org/10.1351/pac198961061165
- Żuk M., Kulma A., Dymińska L., Szołtysek K., Prescha A., Hanuza J., Szopa J., 2011. Flavonoid engineering of flax potentiate its biotechnological application. BMC Biotechnol. 11, 10, https://doi.org/10.1186/1472-6750-11-10
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Bibliografia
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