Identification of dehydration responsive genes from two non-nodulated alfalfa cultivars using Medicago truncatula microarrays

• Autorzy: Chen D. , Liang M.-X. , DeWald D. , Weimer B. , Peel M. D. , Bugbee B. , Michaelson J. , Davis E. , Wu Y.
• Języki publikacji: EN

Abstrakty

EN

To have a comprehensive understanding of how legume plants respond to drought at the gene expression level and examine whether legume plants that are not fixing nitrogen would behave similar to non-legume plants in drought response, transcriptomes were studied in two nonnodulated alfalfa (Medicago sativa L.) cultivars, Ladak and 53V08, when plants were subjected to dehydration stress. Two heat shock-related protein genes were up-regulated in the 3-h stressed shoots in both cultivars. One of them was also up-regulated in the 8-h stressed shoots, along with dehydrin and LEA. A xyloglucan endotransglycosylase and a gene with unknown function were down-regulated in both 3- and 8-h stressed shoots. In roots, nearly half of the 55 genes commonly up-regulated at 3 h are involved in pathogen resistance, insect defense and flavonoid synthesis, which differs from other dehydration-responsive transcriptomes in the literature. Many known drought-responsive genes, such as LEA and dehydrin, were up-regulated after 8 h of treatment. The genes encoding caffeoyl-CoA O-methyl transferase and dirigent were up-regulated in the 3-h stressed roots, while two aquaporin genes were down-regulated, suggesting that lignification and prevention of water loss in roots in initial dehydration stress is a common strategy for both cultivars. The results also indicate the involvement of some specific signal transduction pathways, osmotic adjustment and ion homeostasis regulation during dehydration response. Besides those known dehydration-responsive genes in the literature, some dehydration responses and genes in alfalfa appear to be unique. Our results provide valuable insight into a comprehensive understanding of dehydration response in alfalfa at the molecular level.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2008
• Tom: 30
• Numer: 2
• Opis fizyczny: p.183-199,fig.,ref.

Twórcy

autor

Chen D.

• Center for Integrated BioSystems, Utah State University, Logan, UT 84322, USA

autor

Liang M.-X.

• Department of Plant Resource Science, Faculty of Agriculture, Kobe University, 1-1 Rokkodai-cho, Nada-ku, 657-8501 Kobe, Japan
• Department of Plants, Soils, and Climate, Utah State University, Logan, UT 84322, USA

autor

DeWald D.

• Center for Integrated BioSystems, Utah State University, Logan, UT 84322, USA
• Department of Biology, Utah State University, Logan, UT 84322, USA

autor

Weimer B.

• Center for Integrated BioSystems, Utah State University, Logan, UT 84322, USA
• Department of Nutrition and Food Sciences, Utah State University, Logan, UT 84322, USA

autor

Peel M. D.

• USDA-ARS Forage and Range Research Laboratory, Utah State University, Logan, UT 84322, USA

autor

Bugbee B.

• Department of Plants, Soils, and Climate, Utah State University, Logan, UT 84322, USA

autor

Michaelson J.

• Center for Integrated BioSystems, Utah State University, Logan, UT 84322, USA

autor

Davis E.

• Department of Plants, Soils, and Climate, Utah State University, Logan, UT 84322, USA

autor

Wu Y.

• Department of Plants, Soils, and Climate, Utah State University, Logan, UT 84322, USA

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