Warianty tytułu
Języki publikacji
Abstrakty
One of the methods which enhances production of plant secondary metabolites including alkaloids is the use of abiotic elicitors in vitro. The aim of the study was the employment of aluminum (Al) as an abiotic elicitor in such concentrations, which were not stressful for the plant, to avoid intense growth limiting, but could elicit tropane alkaloid biosynthesis. The in vitro propagated plantlets of Datura innoxia were exposed to different concentrations of AlCl3 (0, 25, 75 and 225 µM) to determine its effects on hyoscyamine and scopolamine contents in roots and shoots, which were analyzed by HPLC. Antioxidant enzyme activities were determined by spectrophotometer. Results showed that aluminum reduced shoots’ and roots’ fresh weights and the reduction in roots were more than the shoots. In addition, Al had significant positive effects on hyoscyamine and scopolamine contents especially in roots of the plantlets and AlCl₃ caused ROS production in shoots. These findings suggest that aluminum can likely elicit the tropane alkaloid biosynthesis in D. innoxia plantlets in vitro.
Słowa kluczowe
Wydawca
Czasopismo
Rocznik
Tom
Numer
Opis fizyczny
p.1035-1041,fig.,ref.
Twórcy
autor
- Medicinal Plant Research Center and Department of Biology, Faculty of Sciences, Shahed University, Tehran, Iran
autor
- Medicinal Plant Research Center and Department of Biology, Faculty of Sciences, Shahed University, Tehran, Iran
Bibliografia
- Ajungla L, Patil PP, Barmukh RB, Nikam TD (2009) Influence of biotic and abiotic elicitors on accumulation of hyosyamine and scopolamine in root culture of Datura metel L. Indian J Biotech 8:317–322
- Alvarez SIP, Spollansky TC, Giulietti AM (2000) The influence of different biotic and abiotic elicitors on the production and profile of tropane alkaloids in hairy root cultures of Brugmansia candida. Enzy Mic Technol 26:252–258
- Apel K, Hirth H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–99
- Becana M (2007) Oxidative stress as a response to salinity and aluminum. Lotus Newsl 37:98
- Bradford M (1976) A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72:248–254
- Cakmak I, Horst WJ (1991) Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase and peroxidase activities in root tips of soybean (Glycine max). Physiol Plant 83:463–468
- Dazy M, Masfaraud JF, Ferard JF (2009) Induction of oxidative stress biomarkers associated with heavy metal stress in Fontinalis antipyretica Hedw. Chemosphere 75:297–302
- Dechaux C, Boitel-Conti M (2005) A strategy for overaccumulation of scopolamine in Datura Innoxia hairy root cultures. Acta Biol Cracov Bot 47:101–107
- Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirement of suspension cultures of soybean root cells. Exp Cell Res 50:151–158
- Giannakoula A, Moustakasa M, Syrosb T, Yupsanisb T (2010) Aluminum stress induces up-regulation of an efficient antioxidant system in the Al-tolerant maize line but not in the Alsensitive line. Env Exp Bot 67:487–494
- Giannopolitis CN, Ries SK (1977) Superoxide dismutase. I. Occurrence in higher plants. Plant Physiol 59:309–314
- Guo T, Zhang G, Zhou M, Wu F, Chen J (2004) Effects of aluminum and cadmium toxicity on growth and antioxidant enzyme activities of two barley genotypes with different Al resistance. Plant Soil 258:241–248
- Hafis C, Romero-puertas MC, del Rio LA, Abdelly C, Sandalio LM (2011) Antioxidative response of Hordeum maritimum L. to potassium deficiency. Acta Physiol Plant 33:193–202
- Hamel F, Breton Ch, Houde M (1998) Isolation and characterisation of wheat aluminium-regulated genes: possible involvement of aluminium as a pathogenesis response elicitor. Planta 205:531–538
- Hashimoto T, Yamada Y (1986) Hyoscyamine 6ß-hydroxylase, a 2-oxoglutarate-Dependent dioxygenase, in alkaloid-producing root cultures. Plant Physiol 81:619–625
- Konarska A (2010) Effects of aluminum on growth and structure of red pepper (Capsicum annuum L.) leaves. Acta Physiol Plant 32:145–151
- Mano Y, Nabeshima S, Matsui C, Ohkawa H (1986) Production of tropane alkaloids by hairy root cultures of Scopolia japonica. Agric Biol Chem 50:2715–2722
- Mossor-Pietraszewska T (2001) Effect of aluminum on plant growth and metabolism. Acta Biochim Pol 48:673–686
- Palazon J, Moyano E, Cusidó RM, Bonfill M, Oksman-Caldentey KM, Pinol MT (2003) Alkaloid production in Duboisia hybrid hairy roots and plants overexpressing the h6h gene. Plant Sci 165:1289–1295
- Panda SK, Singha LB, Khan MH (2003) Does aluminium phytotoxicity induce oxidative stress in greengram? Bulg J Plant Physiol 29:77–86
- Pereira LB, Tabaldi LA, Gonc¸alves JF, Jucoski GO, Pauletto MM, Weis S, Nicoloso FT, Borher D, Rocha JBT, Schetinger MRC (2006) Effect of aluminum on d-aminolevulinic acid dehydratase (ALA-D) and the development of cucumber (Cucumis sativus). Env Exp Bot 57:106–115
- Radic S, Babic M, Skobic D, Roje V, Pevalek-Kozlina B (2009) Ecotoxicological effects of Aluminum and zinc on growth and antioxidants in Lemna minor. Ecotox Environ Safe 10:1016–1022
- Sarowar S, Kim E, Kim Y, Ok S, Kim K, Hwang B, Shin J (2005) Overexpression of a pepper ascorbate peroxidase-like 1 gene in tobacco plants enhances tolerance to oxidative stress and pathogens. Plant Sci 169:55–63
- Spollansky T, Pitta-Alvarez S, Giulietti A (2000) Effect of jasmonic acid and aluminum on production of tropane alkaloids in hairy root cultures of Brugmansia candida. J Biotech 3:72–75
- Yamamoto Y, Kobayashi Y, Matsumoto H (2001) Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibition in pea roots. Plant Phys 125:199–208
- Zarate R, Hermosin B, Cantos M, Troncoso A (1997) Tropane alkaloid distribution in Atropa baetica Plants. J Chem Ecol 23:2059–2066
- Zayed R, Wink M (2004) Induction of tropane alkaloid formation in transformed root cultures of Brugmansia suaveolens (Solanaceae). Z Naturforsch 59c:863–867
Uwagi
Rekord w opracowaniu
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-dcf91351-d1f4-45b9-a444-b97fca2d37a6