Czasopismo
Tytuł artykułu
Warianty tytułu
Study on the usefulness of seroneutralization tests in the diagnostics of infectious haematopoietic necrosis (IHN)
Języki publikacji
Abstrakty
The purpose of the project was to study the correlation between the presence of the IHN virus and the presence of antibodies against this virus in fish sera. Experimental infection of rainbow trout was performed at temperatures of 12°C±1 and 15°C±1. The trouts’ sera were collected at 7-day intervals. Samples from farms naturally infected with IHNV were also tested. The presence of antibodies against IHNV was determined by seroneutralization and the virus was detected by isolation in cell culture. It was found that in the experimentally infected rainbow trout, antibodies against IHN were found earlier in the higher temperature, after 21 days post infection, whereas the presence of IHNV was observed to last longer in the lower temperature. In the study performed on 30 samples of sera from IHN-infected farms, antibodies were detected in 12 samples. Detection of antibodies against IHNV in fish serum can be a helpful tool supporting recommended techniques.
Wydawca
Czasopismo
Rocznik
Tom
Numer
Opis fizyczny
s.751-754,rys.,tab.,bibliogr.
Twórcy
autor
- Zakład Chorób Ryb, Państwowy Instytut Weterynaryjny - Państwowy Instytut Badawczy, Al.Partyzantów 57, 24-100 Puławy
autor
autor
autor
autor
autor
Bibliografia
- 1.Biuletyn Głównego Inspektoratu Weterynarii. Stan chorób zakaźnych zwierząt 2008-2011, Warszawa.
- 2.Burke J., Grischkowsky R.: An epizootic caused by infectious hematopoietic necrosis virus in an enhanced population of sockeye salmon, Oncorhynchus nerka (Walbaum), smolts at Hidden Creek, Alaska. J. Fish Dis. 1984, 7, 421-429.
- 3.Decyzja Komisji 2001/183/WE z dnia 22 lutego 2001 r. ustanawiająca plany pobierania próbek i metody diagnostyczne do celów wykrywania i potwierdzania występowania niektórych chorób ryb oraz uchylająca decyzje 92/532/EWG.
- 4.Dyrektywa Rady 2066/88/WE z dnia 24 października 2006 r. w sprawie wymogów w zakresie zdrowia zwierząt akwakultury i produktów akwakultury oraz zapobiegania niektórym chorobom zwierząt wodnych i zwalczania tych chorób.
- 5.Enzmann P. J., Konrad M.: Antibodies against VHS in whitefish of the Lake of Constance, West Germany. Bull. Eur. Assoc. Fish Pathol. 1990, 10, 24-25.
- 6.Enzmann P. J., Konrad M.: Longevity of antibodies in brown trout and rainbow trout following experimental infection with VHS-virus. Bull. Eur. Assoc. Fish Pathol. 1993, 13, 193-194.
- 7.Fregeneda-Grandes J. M., Olesen N. J.: Detection of rainbow trout antibodies against viral haemorrhagic septicaemia virus (VHSV) by neutralisation test is highly dependent on the virus isolate used. Dis. of Aquat. Org. 2007, 74, 151-158.
- 8.Hattenberger-Baudouy A. M., Dalton M., Merle G., Kinkelin P. de: Serum neutralization test for epidemiological studies of salmonid rhabdoviroses in France. Vet. Res. 1995, 26, 512-520.
- 9.Jørgensen P. E. V., Olesen N. J., Lorenzen N., Winton J. R., Ristow S.: Infectious hematopoietic necrosis (IHN) and Viral hemorrhagic septicemia (VHS): detection of trout antibodies to the causative viruses by means of plaque neutralization, immunofluorescence, and enzyme-linked immunosorbent assay. J. Aquat. Anim. Health 1991, 3, 100-108.
- 10.Lorenzen N., Lapatra S. E.: Immunity to rhabdoviruses in rainbow trout: the antibody response. Fish Shellfish Immunol. 1999, t. 9, nr 4, 345-360.
- 11.Lorenzen N., Olesen N. J., Jørgensen P. E. V.: Antibody response to VHS virus proteins in rainbow trout. Fish Shellfish Immunol. 1993, 3, 461-473.
- 12.O.I.E., Światowa Organizacja Zdrowia Zwierząt: International Aquatic Animal Health Code, 2011, 14 ed.
- 13.Olesen N. J., Lorenzen N., Jørgensen P. E. V.: Detection of rainbow trout antibody to Egtved virus by enzyme-linked immunosorbent assay (ELISA), immunofluorescence (IF), and plaque neutralization tests (50% PNT). Dis. Aquat. Org. 1991, 10, 31-38.
- 14.St-Hilaire S., Ribble C., Traxler G., Davies T., Kent M. L.: Evidence for a carrier state of infectious hematopoietic necrosis virus in chinook salmon Oncorhynchus tshawytacha. Dis. of Aquat. Org. 2001, 46, 173-179.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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