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2014 | 36 | 10 |
Tytuł artykułu

Pyramiding Bt genes for increasing resistance of cotton to two major lepidopteran pests: Spodoptera litura and Heliothis armigera

Autorzy
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
To more effectively control two major cotton insects (cotton bollworm and Spodoptera litura) and improve the efficacy of the pest resistance management, novel transgenic plants expressing Bacillus thuringiensis Cry9C gene were generated, and gene stacking strategy was incorporated. Initially, a binary plasmid vector harboring Cry9C gene was introduced into an elite cotton cultivar Simian-3 by Agrobacterium-mediated transformation. Integration and expression of the Cry9C genes in three transgenic lines were confirmed by PCR and RT-PCR. Among these transgenic lines, T0 generation of line 16 (L-16) with normal phenotypes were selected by ELISA assays for its highest expression level of Cry9C. In T1 population of L-16, the expression level of Cry9C ranged from 29 to 45 lg/g fresh leaf. The following insect bioassays demonstrated that transgenic S3-35S::Cry9C cotton plants exhibited moderate toxicity to Heliothis armigera but strong toxicity to S. litura compared with the transgenic plants expressing Cry 1Ac gene. For incorporation of gene staking strategy, Cry9C gene and Cry 2A or Cry 1Ac were pyramided, respectively by sexual crossing. The expression of Cry9C protein in all F1 progenies had a similar level as the parent plants indicating the high heritability of Bt genes in transgenic progenies. Progenies from both Cry9C × Cry 2A and Cry9C × Cry 1Ac exhibited higher resistance to S. litura compared with their parents. Together our data demonstrated that our newly generated transgenic plants represent a reservoir of novel insect-resistant materials in cotton breeding, and the successful incorporation of gene pyramiding technology can provide a new solution of developing multiple resistance management strategies.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
36
Numer
10
Opis fizyczny
p.2717-2727,fig.,ref.
Twórcy
autor
  • National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China
autor
  • National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China
autor
  • National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China
autor
  • National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China
Bibliografia
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  • Cong (2010) Studying on the interaction between Spodoptera litura and transgenic cotton (Cry1Ac). Master D Dissertation, Huazhong Agricultural University
  • Gahan LJ, Ma YT, MacGregor Coble ML, Gould F, Moar WJ, Heckel DG (2005) Genetic basis of resistance to Cry1Ac and Cry2Aa in Heliothis virescens (Lepidoptera: Noctuidae). Journal Econ Entomol 98:1357–1368
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  • Kranthi KR, Naidu S, Dhawad CS, Tatwawadi A, Mate K, Patil E, Bharose AA, Behere GT, Wadaskar RM, Kranthi S (2005) Temporal and intra- plant variability of Cry1Ac expression in Bt-cotton and its influence on the survival of the cotton bollworm, Helicoverpa armigera (Hübner) (Noctuidae: Lepidoptera). Curr Sci 89:291–298
  • Li YH, Romeis J, Wang P, Peng YF, Shelton AM (2011) A comprehensive assessment of the effects of Bt cotton on Coleomegilla maculata demonstrates no detrimental effects by Cry1Ac and Cry2Ab. PLoS ONE 6:e22185
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  • Yi DX, Cui L, Wang L, Liu YM, Zhuang M, Zhang YY, Zhang J, Lang ZH, Zhang ZX, Fang ZY, Yang LM (2013) Pyramiding of Bt cry1Ia8 and cry1Ba3 genes into cabbage (Brassica oleracea L. var. capitata) confers effective control against diamondback moth. Plant Cell Tissue Organ Cult 115(3):419–428
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-d8623892-d3b2-419f-810b-2ca4b7bbe0c0
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