In vitro inhibition of topoisomerase II alpha by reduced glutathione

• Autorzy: Delwar Z. M. , Vita M. F. , Siden A. , Cruz M. , Yakisich J. S.
• Języki publikacji: EN

Abstrakty

EN

 In most cells, the major intracellular redox buffer is glutathione (GSH) and its disulfide-oxidized (GSSG) form. The GSH/GSSG system maintains the intracellular redox balance and the essential thiol status of proteins by thiol disulfide exchange. Topoisomerases are thiol proteins and are a target of thiol-reactive substances. In this study, the inhibitory effect of physiological concentration of GSH and GSSG on topoisomerase IIα activity in vitro was investigated. GSH (0-10 mM) inhibited topoisomerase IIα in a concentration-dependent manner while GSSG (1-100 μM) had no significant effect. These findings suggest that the GSH/GSSG system could have a potential in vivo role in regulating topoisomerase IIα activity.

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2011
• Tom: 58
• Numer: 2
• Opis fizyczny: p.265-267,fig.,ref.

Twórcy

autor

Delwar Z. M.

• Department of Clinical Neuroscience, Karolinska Institute, Stockholm, Sweden

autor

Vita M. F.

autor

Siden A.

autor

Cruz M.

autor

Yakisich J. S.

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Uwagi

PL

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