Use of the rep-PCR technique for differentiating isolates of rhizobacteria

• Autorzy: Lisek A. , Sas Paszt L. , Oskiera M. , Trzcinski P. , Bogumil A. , Kulisiewicz A. , Malusa E.

Warianty tytułu

PL

Zastosowanie techniki rep-PCR do odrozniania izolatow bakterii rizosferowych

• Języki publikacji: EN

Abstrakty

EN

In this study, the rep-PCR technique was used to differentiate isolates of bacteria belonging to genus Pseudomonas and phosphate-dissolving bacteria collected from the root vicinity of apple and sour cherry trees. DNA amplification was carried out with complementary primers for repetitive sequences: REP (repetitive extragenic palindromic sequence), ERIC (enterobacterial repetitive intergenic consensus) and the BOX element. The most differentiated DNA profiles were observed when using REP1R-I and REP2-I primers, in reactions with which 25 different DNA patterns were obtained for 28 isolates. In reactions with the primers ERIC1R and ERIC2 or BOXA1R, 24 and 22 patterns were obtained, respectively. Following the use of all the primers, no differences were found in the DNA profiles of two isolates of Pseudomo­nas bacteria and three isolates of phosphate-dissolving bacteria. This result suggests that the isolates in which no DNA polymorphism was observed belong to the same bacterial strain.

PL

W pracy zastosowano technikę rep-PCR do odróżnienia izolatów bakterii Pseu­domonas oraz bakterii rozpuszczających związki fosforu, pozyskanych z gleby ze strefy korzeniowej drzew jabłoni i wiśni. Amplifikację DNA wykonano z użyciem starterów komplementarnych do sekwencji powtarzalnych: REP (repetitive extragenic palindromic sequence), ERIC (enterobacterial repetitive intergenic consensus) oraz elementu BOX. Najbardziej zróżnicowane profile DNA obserwowano używaj ąc star­terów REP1R-I i REP2-I, w reakcjach z którymi uzyskano 25 wzorów DNA dla 28 izolatów. W reakcjach ze starterami ERIC1R i ERIC2 (24) oraz BOXA1R (22) otrzymano odpowiednio 24 i 22 wzory. Po zastosowaniu wszystkich starterów nie stwierdzono różnic w profilach DNA dwóch izolatów bakterii z rodzaju Pseudomonas oraz trzech izolatów bakterii rozpuszczaj ących związki fosforu. Wynik ten sugeruje, że izolaty, u których nie obserwowano polimorfizmu DNA, należą do tego samego szczepu bakterii.

Słowa kluczowe

EN

beneficial rhizobacteria; rep-polymerase chain reaction; fingerprinting; repetitive sequence; Pseudomonas; bacteria; DNA amplification; enterobacterial repetitive intergenic consensus

• Wydawca: -
• Czasopismo: Journal of Fruit and Ornamental Plant Research
• Rocznik: 2011
• Tom: 19
• Numer: 1
• Opis fizyczny: p.5-12,fig.,ref.

Twórcy

autor

Lisek A.

• Research Institute of Horticulture, Department of Pomology, Pomologiczna 18, 96-100 Skierniewice, Poland

autor

Sas Paszt L.

autor

Oskiera M.

autor

Trzcinski P.

autor

Bogumil A.

autor

Kulisiewicz A.

autor

Malusa E.

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