Micropropagation, antioxidant properties and phytochemical assessment of Swertia corymbosa (Griseb.) Wight ex C. B. Clarke: a medicinal plant

• Autorzy: Mahendran G. , Bai V. N.
• Języki publikacji: EN

Abstrakty

EN

Swertia corymbosa (Griseb.) Wight ex C. B. Clarke, a valuable medicinal plant, has been investigated for its regeneration potential using nodal explants. Out of a range of concentrations of cytokinins [6-benzyl adenine (BA), 6-furfurylaminopurine (Kn), 2-isopentenyl adenine (2iP), thidiazuron (TDZ), and zeatin (Z)] used as supplements with MS, BA at 4.40 μM concentration proved best for multiple shoot induction yielding 26.50 ± 0.26 shoots after 12 weeks of culture. Addition of low concentration of NAA (1.3 μM) in MS medium supplemented with the cytokinin BA (4.40 μM) favoured shoot multiplication. A mean number of 35.78 ± 0.81 shoots were produced per explant. Additive effect of BA (4.40 μM) in combination with Kn (4.64 μM) produced highest number of shoots (83.20 ± 4.29). Addition of GA₃ (1.4 μM) to the above medium not only favored shoot elongation but also enhanced the number of shoots (113.98 ± 3.80). The microshoots were rooted successfully on half-strength MS medium supplemented with 9.8 μM of IBA. The plantlets were successfully transferred to hardening medium containing vermiculite with 87 % survival rate. Screening of the antibacterial, antioxidant activity and estimation of total phenolic and flavonoid content of methanolic extracts of micropropagated plants were also carried out and compared with that of the wild-grown plants. In all the tests, methanolic extract from wild-grown plants showed higher antioxidant, antimicrobial activity, total phenolic and flavonoid content than in vitro propagated plants. The content of secondary metabolites in wild-grown plants and in vitro propagated plants was determined by HPLC coupled with ESI-MS and the presence of loganic acid, swertiamarin, sweroside, gentiopicroside, isovitexin, amoroswertin, amarogentin, gentiacaulein, decussatin, and swertianin in the samples were confirmed. Gentiopicroside (40.726 mg/g) and swertianin (29.598 mg/g) were found to be the major compounds which may be responsible for the antimicrobial and antioxidant activities. The results of the present study confirmed the therapeutic potency of S. corymbosa used in the traditional medicine; in addition, the protocol for in vitro production developed in the present study could be applied for mass multiplication and for the conservation of germplasm.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 03
• Opis fizyczny: p.589-603,fig.,ref.

Twórcy

autor

Mahendran G.

• Plant Tissue Culture Lab, Department of Botany, School of Life Sciences, Bharathiar University, Coimbatore, 641046, Tamilnadu, India

autor

Bai V. N.

• Plant Tissue Culture Lab, Department of Botany, School of Life Sciences, Bharathiar University, Coimbatore, 641046, Tamilnadu, India

Bibliografia

• Ahmad N, Anis M (2011) An efficient in vitro process for recurrent production of cloned plants of Vitex negundo L. Eur J For Res 130:135–144
• Ahmad N, Khan Md I, Ahmed S, Javed SB, Faisal M, Anis M, Rehman S, Umair SM (2013) Change in total phenolic content and antibacterial activity in regenerants of Vitex negundo L. Acta Physiol Plant 35:791–800
• Al-Zubairi AS, Abdul AB, Abdelwahab SI, Yuan Peng C, Mohan S, Elhassan MM (2011) Eleucine indica possesses antioxidant, antibacterial and cytotoxic properties. Evid Based Complement Alternat Med 965370:6. doi:10.1093/ecam/nep091
• Amoo SO, Aremu AO, Van Staden J (2012) In vitro plant regeneration, secondary metabolite production and antioxidant activity of micropropagated Aloe arborescens Mill. Plant Cell Tiss Org Cult 111:345–358
• Anis M, Husain MK, Faisal M, Shahzad A, Ahmad N, Siddique I, Khan H (2009) In vitro approaches for plant regeneration and conservation of some medicinal plants. In: Kumar A, Sopory SK (eds) Recent advances in plant biotechnology and its applications. IK International Pvt Ltd, New Delhi, pp 397–410
• Balaraju K, Agastin P, Ignacimuthu S (2009) Micropropagation of Swertia chirata Buch-Hams. ex Wall. a critically endangered medicinal herb. Acta Physiol Plant 31:487–494
• Barros L, Duenas M, Dias MI, Sousa MJ, Santos-Buelga C, Ferreira ICFR (2013) Phenolic profiles of cultivated, in vitro cultured and commercial samples of Melissa officinalis L. infusions. Food Chem 136:1–8
• Baskaran P, Jayabalan N (2008) Effect of growth regulators on rapid micropropagation and psoralen production in Psoralea corylifolia L. Acta Physiol Plant 30:345–451
• Baskaran P, Singh S, Van Staden J (2013) In vitro propagation, proscillaridin A production and antibacterial activity in Drimia robusta. Plant Cell Tiss Org Cult. doi:10.1007/s11240-013-0322-2
• Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 26:1199–1200
• Bohnert HJ, Nelson DE, Jensen RG (1995) Adaptations to environmental stresses. Plant Cell 7:1099–1111
• Brahmachari G, Monda S, Gangopadhyay A, Gorai D, Mukhopadhyay B, Saha S, Brahmachari AK (2004) Swertia (Gentianaceae): chemical and pharmacological aspects. Chem Biodiver 1:1627–1651
• Branka V, Dijana KM, Teodora J, Snezana ZK, Dragan V (2013) Quantitative determination of secoiridoid and xanthone glycosides of Gentiana dinarica Beck cultured in vitro. Acta Physiol Plant 35:567–574
• Carnat A, Fraisse D, Carnat A-P, Felgines C, Chaud D, Lamaison J-L (2005) Influence of drying mode on iridoid bitter constituent levels in gentian root. J Sci Food Agr 85:598–602
• Chandrasekhar T, Hussain MT, Jayanand B (2005) In vitro micropropagation of Boswellia ovalifoliolata. Z Nat 60:505–507
• Chaudhuri RK, Pal A, Jha TB (2007) Production of genetically uniform plants from nodal explants of Swertia chirata Buch.-Ham. ex Wall. an endangered medicinal herb. In Vitro Cell Dev Biol Plant 43:467–472
• Chhetri DR, Parajuli P, Subba GC (2005) Ant diabetic plants used by Sikkim and Darjeeling Himalayan tribes, India. J Ethnopharmacol 99:199–202
• Danova K, Nikolova-Damianova B, Denev R, Dimitrov D (2012) Influence of vitamins on polyphenolic content, morphological development, and stress response in shoot cultures of Hypericum spp. Plant Cell Tiss Org Cult 110:383–393
• Dinnis TCP, Madeira VMC, Almeida LM (1994) Action of Queryphenolic derivatives (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 315:161–169
• Dorman HJD, Peltoketo A, Hiltunen R, Tikkanen MJ (2003) Characterisation of the antioxidant properties of deodorized aqueous extracts from selected Lamiaceae herbs. Food Chem 83:255–262
• Faisal M, Siddique I, Anis M (2006) An efficient plant regeneration system for Mucuna pruriens L. (DC.) using cotyledonary node explants. In Vitro Cell Dev Biol Plant 42:59–64
• Gadidasu K, Umate P, Aileni M, Kota SR, Kokkirala VR, Kasula K, Abbagani S (2011) Micropropagation of a valuable ethnomedicinal plant Streblus asper Lour. J Phytol 3:18–23
• Garcia-Perez E, Gutierrez-Uribe JA, Garcia-Lara S (2012) Luteolin content and antioxidant activity in micropropagated plants of Poliomintha glabrescens (Gray). Plant Cell Tiss Org Cult 108:521–527
• Guimaraes R, Sousa MJ, Ferreira ICFR (2010) Contribution of essential oils and phenolics to the antioxidant properties of aromatic plants. Ind Crops Prod 32:152–156
• Hostettmann K, Hostettmann M (1989) Xanthones. In: Dey PM, Harborne JB (eds) Methods in plant biochemistry-vol I Plant Phenolics. Academic Press, London, pp 493–508
• Indra D, Bhatt, Dhar U (2000) Combined effect of cytokinins on multiple shoot production from cotyledonary node explants of Bauhinia vahlii. Plant Cell Tiss Org Cult 62:79–83
• Joshi SC, Verma AR, Mathela CS (2010) Antioxidant and antibacterial activities of the leaf essential oils of Himalayan Lauraceae species. Food Chem Toxicol 48:37–40
• Khateeb WA, Hussein E, Qouta L, Aludatt M, Al-Shara B, Abuzaiton A (2012) In vitro propagation and characterization of phenolic content along with antioxidant and antimicrobial activities of Cichorium pumilum Jacq. Plant Cell Tiss Org Cult 110:103–110
• Li WL, Zheng HC, Bukuru J, De Kimpe N (2004) Natural medicines used in the traditional Chinese medical system for therapy of diabetes mellitus. J Ethnopharmacol 92:1–21
• Liu L, Zou L, Ma Y, Chen WH, Wang B, Xu ZL (2006) Synthesis and pharmacological activities of xanthene derivatives as α-glucosidase inhibitors. Bioorg Med Chem 14:5683–5690
• Lystvan K, Belokurova V, Sheludko Y, Ingham JL, Prykhodko V, Kishchenko O, Paton E, Kuchuk M (2010) Production of bakuchiol by in vitro systems of Psoralea drupacea Bge. Plant Cell Tiss Org Cult 101:99–103
• Mahendran G, Lavanya Devi K, Narmatha Bai V (2006) Micropropagation of Spilanthes paniculata Wall ex DC. J Plant cell Biotech Mol Boil 7:85–88
• Mahendran G, Manoj M, RajendraPrasad KJ, NarmathaBai V (2013) Evaluation of anti-inflammatory and anti-noceceptive activity of xanthones from Swertia corymbosa (Griseb.) Wight ex C.B.Clarke. Int J Pharm Pharm Sci 5(3):459–463
• Matkowski A (2008) Plant in vitro culture for the production of antioxidants—a review. Biotech Adv 26:548–560
• Menkovic N, Savikin-Fodulovic K, Vinterhalter B, Vinterhalter D, Jankovic T, Krstic D (2000) Secoiridoid content in hairy roots of Gentiana punctata. Pharm Pharmacol Lett 2:73–75
• Mohanty S, Parida R, Singh S, Joshi R, Subudhi E, Nayak S (2011) Biochemical and molecular profiling of micropropagated and conventionally grown Kaempferia galanga. Plant Cell Tis Org Cult 106:39–46
• Morgan ER, Butler RM, Bicknell RA (1997) In vitro propagation of Gentiana cerina and Gentiana corymbifera. New Zeal J Crop Hort Sci 25:1–8
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco cultures. Physiol Plant 15:473–497
• Muruganandan S, Srinivasan K, Gupta S, Gupta PK, Lal J (2005) Effect of mangiferin on hyperglycemia and atherogenicity in streptozotocin diabetic rats. J Ethnopharmacol 97:497–501
• Nikam TD, Ghorpade RP, Nitnaware KM, Ahire ML, Lokhande VL, Chopra A (2013) Micropropagation and non-steroidal anti-inflammatory and anti-arthritic agent boswellic acid production in callus cultures of Boswellia serrata Roxb. Physiol Mol Biol Plants 19:105–116
• Parsaeimehr A, Sargsyan E, Javidnia K (2010) A comparative study of the antibacterial, antifungal and antioxidant activity and total content of phenolic compounds of cell cultures and wild plants of three endemic species of Ephedra. Molecules 15:1668–1678
• Pinto MMM, Sousa ME, Nascimento MSJ (2005) Xanthone derivatives: new insights in biological activities. Curr Med Chem 12:2517–2538
• Pulido R, Bravo L, Sauro-Calixto F (2000) Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. J Agr Food Chem 48:3396–3402
• Rainha N, Koci K, Coelho AV, Lima E, Baptista J, FernandesFerreira M (2013) HPLC–UV–ESI-MS analysis of phenolic compounds and antioxidant properties of Hypericum undulatum shoot cultures and wild-growing plants. Phytochemistry 86:83–91
• Rajanna LN, Sharanabasappa G, Seetharam YN, Aravind B, Mallikharjuna PB (2011) In vitro regeneration of cotyledonary node explant of Bauhinia racemosa. Bot Res Int 4:75–80
• Ram K, Shekhawat NS (2011) Micropropagation of commercially cultivated Henna (Lawsonia inermis) using nodal explants. Physiol Mol Biol Plants 17:281–289
• Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231–1237
• Sacchetti G, Maietti S, Muzzoli M, Scaglianti M, Manfredini S, Radice M, Bruni R (2005) Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods. Food Chem 91:621–632
• Siddhuraju P, Becker K (2003) Studies on antioxidant activities of Mucuna seed (Mucuna pruriens var. utilis) extracts and certain non-protein amino/imino acids through in vitro models. J Sci Food Agr 83:1517–1524
• Siddhuraju P, Becker K (2007) The antioxidant and free radical scavenging activities of processed cowpea (Vigna unguiculata (L.) Walp.) seed extracts. Food Chem 101:10–19
• Siddique I, Anis M (2006) Thidiazuron induced high frequency shoot bud formation and plant regeneration from cotyledonary node explants of Capsicum annuum L. Indian J Biotech 5:303–308
• Siddique I, Anis M (2007) In vitro shoot multiplication and plantlet regeneration from nodal explants of Cassia angustifolia (Vahl.): a medicinal plant. Acta Physiol Plant 29:233–238
• Siddique I, Anis M (2008) An improved plant regeneration system and ex vitro acclimatization of Ocimum basilicum L. Acta Physiol Plant 30:493–499
• Siddique I, Anis M, Jahan AA (2006) Rapid multiplication of Nyctanthes arbor-tristis L. through in vitro axillary shoot proliferation. World J Agric Sci 2:188–192
• Silva EM, Souza JNS, Rogez H, Rees JF, Larondella Y (2006) Antioxidant activities and polyphenolic contents of fifteen selected plant species from the Amazonian region. Food Chem 101:1012–1018
• Sowndhararajan K, Kang SC (2013) Free radical scavenging activity from different extracts of leaves of Bauhinia vahlii Wight and Arn. Saudi J Biol Sci. doi:10.1016/j.sjbs.2012.12.005
• Szucs Z, Danos B, Nyiredy S (2002) Comparative analysis of the underground parts of Gentiana species by HPLC with diodearray and mass spectrometric detection. Chromatographia 56(1):19–22
• Thenmozhi K, Manian S, Paulsamy S (2013) Antioxidant and free radical scavenging potential of leaf and stem bark extracts of Bauhinia malabarica Roxb. Int J Pharm Pharm Sci 5:306–311
• Tian LY, Bai X, Chen XH, Fang JB, Liu SH, Chen JC (2010) Anti diabetic effect of methylswertianin and bellidifolin from Swertia punicea Hemsl. and its potential mechanism. Phytomedicine 17:533–539
• Verpoorte R, Memelink J (2002) Engineering secondary metabolite production in plants. Curr Opin Biotech 13:181–187
• Vinterhalter B, Jankovic T, Savikin K, Nikolic R, Vinterhalter D (2008) Propagation and xanthone content of Gentianella austriaca shoot cultures. Plant Cell Tiss Org Cult 94:329–335
• Wang L, An L, Hu Y, Wei L, Li Y (2009) Influence of phytohormones and medium on the shoot regeneration from leaf of Swertia chirata Buch.-Ham. ex wall. in vitro. Afr J Biotech 8:2513–2517
• Wu HJ, Wang XX, Li Y, Zhang DG, Zhang B, Wang XY (2011) Propagation of Gentiana macrophylla (Pall) from hairy root explants via indirect somatic embryogenesis and gentiopicroside content in obtained plants. Acta Physiol Plant 33:2229–2237
• Yanishlieva NV, Marinova E, Pokorny J (2006) Natural antioxidants from herbs and spices. Eur J Lipid Sci Tech 108:776–793
• Zhang Z, Leung DWM (2002) Factors influencing the growth of micropropagated shoots and in vitro flowering of Gentian. Plant Growth Regul 36:245–251
• Zhang HL, Xue SH, Pu F, Tiwari RK, Wang XY (2010) Establishment of hairy root lines and analysis of gentiopicroside in the medicinal plant Gentiana macrophylla. Russ J Plant Physiol 57:110–117
• Zhao J, Hu Q, Guo Y-Q, Zhu W-H (2001) Effects of stress factors, bioregulators, and synthetic precursors on indole alkaloid production in compact callus clusters cultures of Catharanthus roseus. Appl Microbiol Biotech 55:693–698
• Zhiguo Z, Xianzhong H, Zhiguang C, Hua L, Li W, Xin H (1993) Gentiopiroside production from roots cultures of Gentiana manshurica. J Plant Physiol Mol Biol 19:66–70
• Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64:555–559

Identyfikatory

DOI

10.1007/s11738-013-1435-2